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ProGlyProt ID
BC131
Organism Information
Organism Name
Clostridium difficile BI-1
Domain
Bacteria
Classification
Family: Clostridiaceae
Order: Clostridiales
Class: Clostridia
Division or phylum: "Firmicutes"
Taxonomic ID (NCBI)
 
 
Genome Sequence(s)
EMBL
Organism Additional Information
Clostridium difficile is a gram-positive, anaerobic, spore-forming bacterium that produces TcdA and TcdB toxins causing severe tissue damage. It ia an opportunistic pathogen that is the major cause of antibiotic-associated diarrhoea and pseudomembranous colitis in humans. C. dificile infection can also result in abdominal pain, inflammation, and ulceration of the lining of the intestinal wall.
 
 
Gene Information
Gene Name
fliC
 
 
Protein Information
Protein Name
FliC (Flagellin)
UniProtKB/SwissProt ID
EMBL-CDS
UniProtKB Sequence
Sequence length
290 AA
Subcellular Location
Surface
Function
Forms the filaments of bacterial flagella.
 
 
Glycosylation Status
Glycosylation Type
O (Ser/Thr) linked
Experimentally Validated Glycosite(s) in Full Length Protein
T141, S174, S205
Experimentally Validated Glycosite(s ) in Mature Protein
T141, S174, S205
Glycosite(s) Annotated Protein Sequence
>tr|D0V7J7|D0V7J7_CLODI Flagellin OS=Clostridium difficile GN=fliC PE=4 SV=1 MR
VNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAAGLAISEKMRAQIKGLD QAGRNVQDGISVVQTA
EGALEETGNILQRMRTLSVQSSNETNTAEERQKIADELLQLKDE VERISSSIEFNGKKLLDGSST*(141)E
IRLQVGANFGTNVAGTTNNNNEIKVALVNTSS*(174)IMSKAG ITSSTIASLNADGTSGTDAAKQMVS*(
205)
SLDVALKELNTSRAKLGAQQNRLESTQNNLNNTIE NVTAAESRIRDTDVASEMVNLSKMNILVQASQSM
LAQANQQPQGVLQLLG
Sequence Around Glycosites (21 AA)
NGKKLLDGSSTEIRLQVGANF
EIKVALVNTSSIMSKAGITSS
SGTNAAKQMVSSLDVALKELN
Glycosite Sequence Logo
Glycosite Sequence Logo
Technique(s) used for Glycosylation Detection
Intact mass analysis with QTOF2-MS (hybrid quadrupole time of flight mass spectrometry)
Technique(s) used for Glycosylated Residue(s) Detection
Electron transfer dissociation (ETD) MS
 
 
Glycan Information
Glycan Annotation
Several novel glycan moieties, ranging in mass from 494 Da to 917 Da. HexNAc and methylated deoxyhexose found.
Technique(s) used for Glycan Identification
MS/MS (tandem mass spectrometry), nESI-feCID-MS/MS (nano-electrospray ionization–front-end collision-induced dissociation MS/MS) analyses
 
 
Literature
Reference(s)
1) Twine, S.M., Reid, C.W., Aubry, A., McMullin, D.R., Fulton, K.M., Austin, J. and Logan, S.M. (2009) Motility and flagellar glycosylation in Clostridium difficile. J Bacteriol, 191, 7050-7062. [PubMed: 19749038]
Year of Identification
2009
Year of Validation
2009
 
 
Copyright @ 2011 IMTECH
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