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ProGlyProt ID
BC141
Organism Information
Organism Name
Flavobacterium columnare (formerly Flexibacter columnaris) ATCC 43622
Domain
Bacteria
Classification
Family: Flavobacteriaceae
Order: "Flavobacteriales"
Class: Flavobacteria
Division or phylum: "Bacteroidetes"
Taxonomic ID (NCBI)
 
 
Protein Information
Protein Name
Short glycopeptides
UniProtKB Sequence
Val-Ala-Val-Asp-Ser-Ala-Ala (tripeptides to heptapeptides have been found with a
minimal common fragment of Asp-Ser-Ala)
 
 
Glycosylation Status
Glycosylation Type
O (Ser) linked
Experimentally Validated Glycosite(s) in Full Length Protein
S5
Experimentally Validated Glycosite(s ) in Mature Protein
S5
Glycosite(s) Annotated Protein Sequence
VAVDS*(5)AA
Sequence Around Glycosites (21 AA)
VAVDAA
Technique(s) used for Glycosylation Detection
NMR spectra
Technique(s) used for Glycosylated Residue(s) Detection
ESI Q-TOF-MS (electrospray quadrupole time of flight mass spectrometry) and sequencing
 
 
Glycan Information
Glycan Annotation
Linkage: Man-Ser. The glycan is an unusual hexasaccharide composed of 4-O-methyl-2-acetamido-2-deoxy-D-glucuronic acid (GlcNAcA), D-glucuronic acid (D-GlcA), 2,3-di-O-acetyl-D-xylose (D-Xyl), 2-O-methyl-D-glucuronic acid (D-GlcA), D-mannose (D-Man), and 2-O-methyl-L-rhamnose (L-Rha).
BCSDB ID
Technique(s) used for Glycan Identification
NMR spectra and monosaccharide analysis by GC of alditol acetates.
 
 
Literature
Reference(s)
1) Vinogradov, E., Perry, M.B. and Kay, W.W. (2003) The structure of the glycopeptides from the fish pathogen Flavobacterium columnare. Carbohydr Res, 338, 2653-2658. [PubMed: 14670723]
Additional Comments
The protein could not be identified as per the reference given. Only a heptapeptide was detected.
Year of Identification
2003
Year of Validation
2003
 
 
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