| Glycan : | ProGP ID: |
| Pentasaccharide (HexNAc - HexNAc -HexNAc-HexNAc-HexNAc) | ProGP197, ProGP500, ProGP501, ProGP502, ProGP503, ProGP504, ProGP505, ProGP506, ProGP507 |
| single- and double glucosylated ( glucose and galactose | ProGP546 |
| X-P-HexNAc-HexNAc-Hex-Hex-HexNAc, where X denotes the unknown monosaccharide containing a phosphate group | ProGP344 |
| (398-Da glycan) O-linked HexNAc residue, to which a methylated aspartic acid is linked via a phosphate bond. Flagellins from a number of C. difficile isolates from more recent outbreaks are modified in O linkage with a heterogeneous glycan containing up to five monosaccharide residues with masses of 204 (HexNAc), 146 (deoxyhexose), 160 (methylated deoxyhexose), and 192 (heptose). | ProGP298 |
| 0.69–1.81% carbohydrates | ProGP39 |
| 0.7 pg of hexose per 100 pg of protein. Rhamnose (1.2 mol%), galactose (92.6 mol%), and glucose (6.2 mol%) as hexose, but no pentose. | ProGP157 |
| 0.8% carbohydrate content. | ProGP9 |
| 0.8% galactose, 0.3% glucose, and 0.1% mannose. | ProGP104 |
| 1 or 2 Hex
between
73, 75, 77,
79, 81, 83,
84. | ProGP461 |
| 13% carbohydrate. | ProGP155 |
| 14% carbohydrate content. Repeated tetrasaccharide [→3)-α-D-GalpNAc-(1→3)[α-D-GlcpNAc-(1→2)-β-D-Manp-(1→4)]-α-D-GalpNAc-(1→]n | ProGP91 |
| 15% carbohydrate. | ProGP156 |
| 20% carbohydrate. Carbohydrate analysis revealed the presence of glucose, galactose, and mannose as monomeric units. | ProGP180 |
| 25.7% carbohydrate estimated by phenol-sulfuric acid assay. | ProGP57 |
| 27% carbohydrate. | ProGP138 |
| 39% carbohydrate in rhamnose-rich polysaccharide. GlcNAc-Asn, GalNAc-Asn. | ProGP73 |
| 4% carbohydrates (hexose, pentose, methyl pentose). | ProGP125 |
| 4,6-dideoxy-4-(3-hydroxybutanoylamino)-D-glucose. Protein is glycosylated on at least ten different sites. | ProGP513 |
| 5–6% carbohydrate content, with 20 glucose and mannose, per subunit. 250 pg neutral sugars per mg protein. "High-mannose" or "hybrid-type N-glycosidically-linked carbohydrate chains. Presence of terminal mannose, α( 1-3), α(1-6) or α( 1-2) linked to mannose. | ProGP150 |
| 6 tightly bound sugar residues per polypeptide chain. | ProGP41 |
| 6% carbohydrate. Glucose, galactose, mannose, and ribose were the monomeric units of the carbohydrate component. | ProGP181 |
| 6-deoxy-Tal-Thr. | ProGP322 |
| 6-deoxy-Tal-Thr. Multiple heptoses. | ProGP177 |
| A 376 Da monosaccharide which is pseudaminic acid (Pse5Ac7Ac) modified by the addition of an acetyl group and water. This sugar was detected at three sites in the protein sequence. | ProGP426 |
| A heptasaccharide with sequence 376 Da-Hex-Hex-HexNAc- HexNAc-HexNAc-102 Da, comprised of three N-acetylhexoasmines (with variable addition of 0–2 phosphate groups and 0–2 methyl groups on each), two hexoses and two unknown monosaccharides of 376 and 102 Da. The 376 Da residue is pseudaminic acid (Pse5Ac7Ac) modified by the addition of an acetyl group and water. The number of heptasaccharide chains may vary from one to six, reflecting glycan heterogeneity. | ProGP425 |
| A novel legionaminic acid derivative (417 Da), 7-acetamido-5-(N-methyl-glutam-4-yl)-amino-3,5,7,9-tetradeoxy-D-glycero-α-D-galacto-nonulosonic acid, (αLeg5GluNMe7Ac). | ProGP261 |
| A tetrasaccharide glycoform consisting of di-N-acetylbacillosamine-glucose-di-N-acetyl hexuronic acid-N-acetylhexosamine (diNAcBac-Glc-diNAcHexA-HexNAc). | ProGP497, ProGP517, ProGP518, ProGP519 |
| A total carbohydrate content of 4.5% (by mass) with Gal and GlcNAc as the constituents. Carbohydrate moieties with D-Galf-α(1→3)-D-GlcpNAc-α(1→2)-D-Galf-α(1→2)-[D-Galf-β(1→3)]-D-Gal as the major
component, is O-linked mainly to threonine and partly to serine via galactopyranose. The hydroxyamino-acid-bound galactopyranose residue has probably an α-configuration. The carbohydrate chains appear as clusters located in highly Thr/Pro-rich peptide regions of the glycoproteins. | ProGP86 |
| A trisaccharide composed of one hexose and two N-acetyl-hexosamine residues | ProGP522 |
| All 32 sites were modified with at least a single hexose, and N1267, N1371, N1638 and N1649 were modified with either a single hexose or a dihexose. | ProGP515 |
| An oligosaccharide with the structure 4-Me-β-ManpNAcCONH2-(1→3)-[Pse5Am7Gc-(2→4)-]-β-ManpNAcA-(1→4)-[4-Me-α-Galp-(1→2)-]-α-Fucp-(1→4)-[-α-Xylp-(1→3)-]-β-GlcpA-(1→3)-[-β-Digp-(1→2)-]-α-Galp. | ProGP352, ProGP353, ProGP354, ProGP403, ProGP409, ProGP410 |
| Approx. 10% (wt/wt) total carbohydrate is present in GspB105 and approx. 9% (wt/wt) carbohydrate in GspB194. Carbohydrates contain N-acetylglucosamine and some amounts of glucose or mannose. | ProGP230 |
| At least three Ser-linked oligosaccharide species with one of them having N-acetylgalactosamine at the reducing end, rhamnose and an unknown component, are present. | ProGP112 |
| Branched sulfated heptasaccharide, Hex4(GlcNAc)2 plus sulfoquinovose where Hex is D-mannose and D-glucose. | ProGP52, ProGP428, ProGP429, ProGP430, ProGP431, ProGP432, ProGP433, ProGP434, ProGP435, ProGP436, ProGP437, ProGP438 |
| C-terminal L-alaninol is glycosylated by an O-methylated rhamnosyl residue (Rha) and the D-allo-Thr is linked to a 6-deoxytalose (6-dTal). | ProGP208 |
| Carbohydrate content 8% corresponding to 40 to 50 sugar molecules per protein monomer. Acidic, repetitive heterogenous glycans of varying length containing N-acetylglucosamine, 1,6 linked glucose, 1,4 linked galactosamine and/or unidentified sugar-related component. | ProGP55 |
| Carbohydrate content between 8 and 16% (mainly cellohexaose). | ProGP42 |
| Carbohydrate moiety (~13.5% of the weight of the VGP) is primarily comprised of neutral sugars and smaller amounts of hexosamines and uronic acids. Terminal N-acetylgalactosamine residues. | ProGP38 |
| Carbohydrates (glucose, mannose, and glucosamine). Both N and O linked glycans present. | ProGP109 |
Carbohydrates present: glucose, mannose, and glucosamine. Both N and O linked glycans present.
β-elimination revealed that at least 15 carbohydrate chains are bound to Ser or Thr. Two molecules of 150 KDa proteases are associated with glycosylated tetrabrachion polypeptides (85 KDa & 92KDa) and for this tetrabrachion-protease complex overall carbohydrate content is 38%. | ProGP108 |
| Contains 10% w/w sugars N-acetylgalactosamine and galactose. | ProGP93 |
| Contains mannose, glucose, galactose and rhamnose as revealed by neutral sugar and amino sugar analysis. These sugars are present in a molar ratio of 1.85, 1, 1.02 and 0.78, respectively. Glycan chains are bound via O-linkage to serine or threonine residues. | ProGP209 |
| Contains N-linked glycans. | ProGP117 |
| Contains rhamnose, 3-O-methyl rhamnose, and galactosamine. | ProGP247 |
| D-Araf (D-arabinofuranose). | ProGP262 |
| Exogenous fucose | ProGP311 |
| Exogenous fucose. | ProGP312, ProGP313, ProGP314, ProGP315, ProGP317, ProGP318, ProGP319, ProGP355, ProGP356, ProGP357 |
| Galactosamine, galactose, glucosamine, glucose, mannose with molar ratios 1.0, 1.7, 2.0, 2.7, 4.0, respectively. | ProGP146 |
| Galactose and mannose are the constituents of the oligosaccharide that were identified by GCMS. | ProGP234 |
| GalNAc-α1,3-bacillosamine. | ProGP244 |
| GlcNAc | ProGP453, ProGP514 |
| GlcNAc with hexose sugar | ProGP316 |
| GlcNAc-Asn linkage. Branched glycan largely based on mannose subunits yet also containing glucose and galactose moieties. | ProGP8 |
| Glucose and mannose as main monosaccharide residues. | ProGP301 |
| Glucose moieties. | ProGP212 |
| Glucose monomers and glucose oligosaccharides. | ProGP15 |
| Glycan has not been characterized but identified to be a hexasaccharide of 1156 Da | ProGP415 |
| Glycan identified to be 291 Da in size and possessing acetamidino group and ammonia. | ProGP413 |
| Glycan primarily contains xylose. | ProGP253 |
Glycan represents 10% of the total mass of the protein.
Major glycan is pseudaminic acid and its derivatives, Pse5Pr7Pr, Pse5Ac7Ac8OAc, Pse5Am7Ac.
Pse5Ac7Ac (5,7-diacetamido-3,5,7,9 - tetradeoxy-L-glycero-L-manno- nonulosonic acid), with 5-acetamidino pseudaminic acid (Pse5Am7Ac) and 5,7-N-(2,3-dihydroxyproprionyl)-pseudaminic acid (Pse5Pr7Pr) are also present. In addition, novel glycans, Pse5Am7Ac8GlnAc and Pse5Ac7Ac8OAc, have also been found. S398 and S405 carry Pse5Pr7Pr moiety while T394, S401 and S409 are modified with Pse5Ac7Ac residues. Pse5Ac7Ac is also written as Pse5NAc7NAc (Pse). Microheterogeneity in glycosylation has been observed. | ProGP207 |
| Glycan represents 2.1% (wt/wt) of the molecule. It is composed almost entirely of xylose (89.6%). Glucose (6.9%), mannose (2.2%), and rhamnose (1.3%) are present in small amounts. 16 xylose residues and one or two glucose or other six-carbon sugar have been calculated per DgpC molecule. Glycosyl linkage analysis has shown that the xylose molecules are largely linked 1,2 and 1,4. | ProGP254 |
| Glycan size identified to be 300 or 342 Da and predicted to be acetylated hexuronic acid. | ProGP412 |
| Glycosylated by GlcNAc-containing oligosaccharides in E. coli. | ProGP246 |
| Glycosylated by α-mannose residues exclusively. | ProGP439 |
| Glycosylation does not seem to have any effect on antifreez activity while its removal decreases the ice-nucleation activity. The protein is relatively rich Gly and Ala. | ProGP142, ProGP143 |
| Heptasaccharide | ProGP424 |
| Heptasaccharide GalNAc- α1,4-GalNAc- α1,4-(Glc β1,3)-GalNAc- α1,4-GalNAc- α1,4-GalNAc- α1,3-Bac- β1 where Bac is bacillosamine (2,4-diacetamido-2,4,6-trideoxyglucopyranose) | ProGP257, ProGP358, ProGP359, ProGP360, ProGP361, ProGP362, ProGP363, ProGP364, ProGP365, ProGP366, ProGP367, ProGP368, ProGP369, ProGP370, ProGP371, ProGP372, ProGP373, ProGP374, ProGP375, ProGP376, ProGP377, ProGP378, ProGP379, ProGP380, ProGP381, ProGP382, ProGP383, ProGP384, ProGP385, ProGP386, ProGP387, ProGP388, ProGP389, ProGP390, ProGP391, ProGP392, ProGP393, ProGP394, ProGP395, ProGP396, ProGP397, ProGP398, ProGP399, ProGP400, ProGP401 |
| Heterogenous multiple heptose residues (atleast 19 per protein molecule). | ProGP202 |
| Hex | ProGP456, ProGP457, ProGP458, ProGP459, ProGP460 |
| Hexasaccharide containing HexNAc-unknown sugar (X)-HexNAc-Hex-Hex-HexNAc (glycan mass 1156-Da ) | ProGP414 |
| Hexasaccharideof 1156 Da and identified sequence is X-P-HexNAc-HexNAc-Hex-Hex-HexNAc, where X denotes the unknown monosaccharide containing a phosphate group. | ProGP343 |
| Hexose and dihexose | ProGP454, ProGP455 |
| It contains rhamnose, galactose, and glucose as neutral sugars and small amounts of glucosamine. | ProGP141 |
| L217C-S-β-Glc(Ac)2, L217C-S-β-Glc(Ac)3, N62C-S-β-Glc(Ac)4, S156C-S-β-Glc(Ac)4, S16C-S-β-Glc(Ac)4. | ProGP198 |
Linkage: Bac-Asn.
1406 Da heptasaccharide composed of GalNAc-α1,4-GalNAc-α1,4-[Glcβ1,3-]GalNAc-α1,4-GalNAc-α1,4-GalNAc-α1,3-Bac-β1,N-Asn-Xaa, where Bac is bacillosamine, 2,4-diacetamido-2,4,6-trideoxyglucopyranose. | ProGP218, ProGP219, ProGP220, ProGP221, ProGP222, ProGP223, ProGP224 |
Linkage: Bac-Asn.
1406 Da heptasaccharide composed of HexNAc-HexNAc-[Hex-]HexNAc-HexNAc-HexNAc-Bac-N-Asn-Xaa, where Bac is bacillosamine, 2,4-diacetamido-2,4,6-trideoxyglucopyranose. | ProGP323 |
Linkage: Bac-Asn.
GalNAc-α-GalNAc-α-GalNAc-α-GalNAc-α-GalNAc-1,3-Bac, where Bac is bacillosamine, 2,4-diacetamido-2,4,6-trideoxyglucopyranose. | ProGP351 |
Linkage: Bac/DATDH-Ser.
Disaccharide composed of a hexose residue linked to a proximal 2,4-diacetamido-2,4,6-trideoxyhexose sugar (HexDATDH). Various X-ray diffraction studies have identified α-D-galactopyranosyl-(1→3)-2,4-diacetamido-2,4-dideoxy-β-D-glucopyranoside (bacillosamine, Bac); Gal-DADDGlc; and GlcNAc-α1,3-Gal, as glycans. DADDGlc has a second acetamido group at C4 of the proximal glucose in place of the hydroxyl group. DADDGlc thus resembles DATDH. However, DATDH lacks the C6 hydroxyl group.
This O-linked glycan may be present as a di- or monosaccharide because of phase variation of select pilin glycosylation genes like pglA. Gal-Gal-DATDH has been shown to be present in some strains. | ProGP119 |
Linkage: DATDH-Ser.
Unusual trisaccharide molecule, Gal(β1-4)Gal(α1-3)2,4-diacetimido-2,4,6-trideoxyhexose [Gal(β1-4)Gal(α1-3)DATDH].
In N. meningitidis 8013 strain, 2-glyceramido 4-acetamido 2,4,6-trideoxyhexose (GATDH) has been found in place of DATDH. | ProGP99 |
Linkage: Deoxyhexose-Ser.
(356 Da) A single deoxyhexose monosaccharide linked to a unique modification of mass 209 Da which contains a phosphate moiety. | ProGP252 |
| Linkage: GalN(Ac)-Ser/Thr. Ara, Rha, Fuc,Man, Gal, Glc, GalN(Ac), GlcN(Ac), and N-acetylneuraminic acid (NANA) totalling 14.4% by weight of protein. The glycan chains are structurally and immunologically related to the cell surface polymer of this Gram-negative bacterium. | ProGP171 |
| Linkage: Gal→Thr. Gal as the major component, Glc, GlcNAc, and an unknown monosaccharide residue were detected. The total carbohydrate content was 6.8% (w/w). Tetrasaccharide is a component of the S1 subunit of the cellulosome (from SDS-PAGE experiments). | ProGP56 |
Linkage: Glc-Asn.
Sulfated oligosaccharides that resemble those of the cell-suface glycoprotein in the bacterium are present. Glucose and glucuronic acid are the constituents of the glycans which are of the type GlcA-(1→4)-GlcA-(1→4)-GlcA-(1→4)-Glc. | ProGP28, ProGP29, ProGP30, ProGP31, ProGP32 |
| Linkage: GlcNAc-Asn. | ProGP245 |
| Linkage: GlcNAc-N-Asn | ProGP70, ProGP101 |
Linkage: Man- Ser.
Branched acidic, heptasaccharide (1244 Da) containing 3 different uronyl analogs (uronic acid derivatives), a methylated Rham and Man, a Glc, and a reducing terminal Man. Only pyranose ring forms were detected [(2-OMe)Man1-4GlcNAcU1-4GlcU1-4Glc1-4(2-OMe)GlcU-4[(2-OMe)Rham1-2]Man]. | ProGP114 |
Linkage: Man- Ser.
Branched acidic, heptasaccharide (1244 Da) containing 3 different uronyl analogs, a methylated Rham and Man, a Glc, and a reducing terminal Man. Only pyranose ring forms were detected [(2-OMe)Man1-4GlcNAcU1-4GlcU1-4Glc1-4(2-OMe)GlcU-4[(2-OMe)Rham1-2]Man]. | ProGP115 |
Linkage: Man- Thr.
Branched, acidic, heptasaccharide (1244 Da) containing 3 different uronyl analogs, a methylated Rham and Man, a Glc, and a reducing terminal Man. Only pyranose ring forms were detected [(2-OMe)Man1-4GlcNAcU1-4GlcU1-4Glc1-4(2-OMe)GlcU-4[(2-OMe)Rham1-2]Man]. | ProGP116 |
| Linkage: Man-O-Thr, Gal-O-Thr. | ProGP26 |
| Linkage: Man-O-Thr, Gal-O-Thr. Terminal α-mannosyl residues with proximal α-galactosyl residues are attached to the T residues. Glucose is also present. | ProGP27 |
| Linkage: Man-Ser. The glycan is an unusual hexasaccharide composed of 4-O-methyl-2-acetamido-2-deoxy-D-glucuronic acid (GlcNAcA), D-glucuronic acid (D-GlcA), 2,3-di-O-acetyl-D-xylose (D-Xyl), 2-O-methyl-D-glucuronic acid (D-GlcA), D-mannose (D-Man), and 2-O-methyl-L-rhamnose (L-Rha). | ProGP229 |
Linkage: Man-Ser.
Branched heptasaccharide is: galactose-β(1–4)[galactose-α(1–3)](2-O-Me)fucose-β(1–4)xylose-β (1–4)glucuronic acid-α(1–2)[rhamnose-α(1–4)]mannose-α(1-O)Ser. | ProGP106 |
Linkage: Man-Thr.
3 mannose units present with Man(1→3)Man linkage. The dominant glycoform identified for MPB83 is Thr48 substituted with a single mannose residue and Thr49 substituted with a Man(1→3)Man linkage. However, evidence for a heterogeneous array of glycoforms from Thr48(Man3) Thr49(Man0) through to Thr48(Man0)Thr49(Man3) has also been observed. | ProGP76 |
Linkage: Man-Thr.
Tetrasaccharide glycan observed in crystal structure: Man-(Rha)-GlcUA-Xyl (xylose-β (1–4)glucuronic acid-α(1–2)[rhamnose-α(1–4)]mannose-α(1-O)Ser. Two more sugars were visible but their density was too weak to be reliably modeled. The branched Rha sugar fills a depression in the protein surface while the remaining sugars extend away from the surface of the protein. | ProGP133 |
Linkage: Rha-Ser.
S201 carries a unique trisaccharide consisting of two rhamnosyl (Rha) residues and one modified 4-amino-4,6-dideoxyglucosyl (Qui4N; named as viosamine) residue, ß-D-Quip4N(3-hydroxy-1-oxobutyl)2Me-(1→3)-α-L-Rhap-(1→2)-α-L-Rhap. The trisaccharide is present as the major glycan on each Ser residue. The glycans contain both L-Rha and D-Rha at a molar ratio of about 4:1.
Heterogeneity due to the addition of one or two units of mass value 147 is also observed. | ProGP226 |
Linkage: Rha-Ser/Thr.
Heterogeneous glycan comprising up to 11 monosaccharide units that are O-linked through a rhamnose (deoxyhexose) residue; rhamnose, mannose, glucose, and 4-amino-4,6-dideoxyglucose (viosamine) as major components with novel, conserved trisaccharide capping. A single rhamnose residue is found at each site in P. aeruginosa JJ692. | ProGP153 |
| Linkage: α-Glc-O-Ser. | ProGP173 |
Linkage: α-L-Rha-Ser.
Tetrasaccharide, 4,6-dideoxy-4-(3-hydroxybutanamido)-2-O-methyl-Glcp-(1→3)-α-L-Rhap-(1→2)-α-L-Rhap-(1→2)-α-L-Rha-(1→, as well as a trisaccharide, 4,6-dideoxy-4-(3-hydroxybutanamido)-2-O-methyl-Glcp-(1→3)-α-L-Rhap-(1→2)-α-L-Rha-(1→. Rhamnose (Rha) is exclusively of the L form. 4,6-dideoxy-4-(3-hydroxy-3-methylbutanamido)-2-O-methylglucopyranose is named as viosamine. Therefore, in the glycans, modified viosamine is present.
The ratios of a trisaccharide and a tetrasaccharide on LC by fluorescence detection were about 5:1, indicating that about five serine residues (including S201) were glycosylated with a trisaccharide. The major component of each glycan is similar to that at S201.
Microheterogeneity in glycosylation is observed. | ProGP227 |
Linkage: αMan-Thr.
34.6% sugar detected.
α-D-Manp(1→2)α-D-Manp (mannobiose), α-D-Manp (single mannose), α-D-Manp(1→2)α-D-Manp(1→2)α-D-Manp (mannotriose) are present. T10 and T18 are glycosylated with mannobiose, T27 with single mannose, and T277 with either of the three. The majority of the antigen species bear six, seven, or eight mannose residues (22, 24, and 17%, respectively), while others three, four, or five mannoses (5, 9, and 14%, respectively). Alpha-D-Man, mannobiose, or mannotriose, Thr residues at positions 10 and 18 were substituted a-D-Manp(132)a-D-Manp, position 27 was substituted with a single a-D-Manp, Thr-277 was substituted with either a-D-Manp, a-D-Manp(132)a-D-Manp, or a-D-Manp(132)a-D-Manp
(132)a-D-Manp. | ProGP51 |
Linkage: β-D-Gal-Ser/Thr.
[→2)-α-L-Rhap-(1→3)-α-D-Fucp-(1→]n=~20. Rhamnose residue at the nonreducing end of the terminal repeating unit of the glycan chain is di-substituted. (R)-N-acetylmuramic acid found in an α-linkage to carbon 3 of the terminal rhamnose residue, serving as capping motif of an S-layer glycan. In addition, that rhamnose is substituted at position 2 with a β-N-acetylglucosamine residue. The S-layer glycan chains are bound via the trisaccharide core →2)-α-L-Rhap-(1→3)-α-L-Rhap-(1→3)-α-L-Rhap-(1→ to carbon 3 of β-D-galactose, which is attached to S and T residues. | ProGP238 |
Linkage: β-D-Galp-Ser/Thr.
[→2)-α-L-Rhap-(1→3)-β-L-Rhap-(1→2)-α-L-Rhap-(1→]n =13–18 (15 on average), with a 2-Omethyl group capping the terminal trisaccharide repeating unit at the non-reducing end of the glycan chains. The glycan chains are bound via the disaccharide (or trisaccharide with additional α-L-Rhap) core→3)-α-L-Rhap-(1→3)-α-L-Rhap-(1→and the linkage glycose β-D-Galp. Approx. 3.6% sugar content detected. Three inherently heterogeneic glycoprotein species of SgsE, 101.66 kDa, 108.68 kDa, and 115.73 kDa, have been determined. Nanoheterogeneity has been assigned to the S-layer glycans, with the most prevalent variation between 12 and 18 trisaccharide repeating units. One, two and three glycosylation sites, respectively, are occupied in these three glycoprotein species. | ProGP18 |
| Linkage: β-D-Glcp-Tyr. β-D-Galf-(1→3)-α-D-Galp- (1→2)-α-L-Rhap-(1→3)-α-D-Manp-(1→3)-α-L- Rhap-(1→3)-β-D-Glcp-(1→4)-L-Tyr. | ProGP88 |
| Linkage: β-Gal-Tyr. Terminal (capping) 3-O-methyl-rhamnose. The glycan chain 3-OMeLRhapα1-[4DManpα1-3LRhapα1]n~27-4DManpα1-3LRhapα1-3LRhapα1-3LRhapα1-3DGalpβ1-O-Tyr, is present. | ProGP105 |
Linkage: β-GalNAc-Asn.
N-linked tetrasaccharide (1036.4 Da) composed of N-acetylgalactosamine, di-acetylated glucuronic acid, an acetylated and acetamidino-modified mannuronic acid linked to threonine, and a novel terminal sugar: Sug-4-β-ManNAc3NAmA6Thr-4-β-GlcNAc3NAcA-3-β-GalNAc-Asn where Sug is a novel monosaccharide unit, (5S)-2-acetamido-2,4-dideoxy-5-O-methyl-α-L-erythro-hexos-5-ulo-1,5-pyranose,. | ProGP259 |
Linkage: β-GalNAc-Asn.
N-linked tetrasaccharide (1036.4 Da) composed of N-acetylgalactosamine, di-acetylated glucuronic acid, an acetylated and acetamidino-modified mannuronic acid linked to threonine, and a novel terminal sugar: Sug-4-β-ManNAc3NAmA6Thr-4-β-GlcNAc3NAcA-3-β-GalNAc-Asn where Sug is a novel monosaccharide unit, (5S)-2-acetamido-2,4-dideoxy-5-O-methyl-α-L-erythro-hexos-5-ulo-1,5-pyranose. | ProGP258, ProGP260 |
| Linkage: β-GalNAc-N-Asn . Glycan moieties account for about 17 mol% of the mature protein. | ProGP78 |
Linkage: β-GalNAc-Ser/Thr.
Hexasaccharide repeating unit (an average of 15 repeats) with the constituents D-rhamnose and 3-N-acetyl-D-fucosamine (3-acetamido-3,6-dideoxy-D-galactose); and β-D-GalNAc-Ser/Thr linkage. An O-methyl group on the non-reducing terminal rhamnose. | ProGP122 |
Linkage: β-GalNAc-Ser/Thr.
Total carbohydrate content of 15%.
Disaccharide repeating unit composed of L-rhamnose and D-glycero-D-manno-heptose residues: →4)-α-L-Rhap-(1→3)-β-D-glycero-D-manno-Hepp-(1→ and β-D-GalpNAc-(1→O)-Ser/Thr linkage. Equal amounts of the complete core structure α-L-Rhap-(1→3)-α-L-Rhap-(1→3)-β-D-GalpNAc-(1→O)-Ser/Thr and the truncated forms α-L-Rhap-(1→3)-β-D-GalpNAc-(1→O)-Ser/Thr and β-D-GalpNAc-(1→O)-Ser/Thr are present. The different cores were substituted with varying numbers of disaccharide repeating units. | ProGP121 |
Linkage: β-GlcNAc-Asn.
64-kDa glycoprotein expresses 17% glycosylation. Total sugar analysis revealed the relative proportions of approximately 7:2:2 for mannose, glucose and GlcNAc.
N-linked hexasaccharide units (at least seven)- two residues each of Man and GlcNAc (one trisubstituted) and one residue each of Glc and 6-deoxy-6-sulfoglucose (6-sulfoquinovose- a rare acidic sugar).
β-D-Glcp-(1→4)-β-D-Quip(6--SO3-)-(1→3)-[α-D-Manp(1→4), α-D-Manp(1→6)] β-D-GlcpNAc-(1→4)-β-D-GlcpNAc-(1→Asn....
O-mannosylation with atleast 35 monosaccharides at unidentified residues. | ProGP151 |
Linkage: β-GlcNAc-Asn.
N-linked heterosaccharide is 17 % of molecular weight of the protein. Composition of the hexasaccharide (1061.83 Da) is α-D-3-O-MetManp-(1→6)-α-D-3-O-MetManp-[(1→2)-α-D-Manp]3-(1→4)-D-GalNAc.
3-O-methylglucose, N-acetylglucosamine, and galactose are also found in lesser amounts. 3-O-methylmannose could be partly replaced by 3-O-methylglucose.
3-O-methylmannose and 3-O-methylglucose are present in the intact glycoprotein in a molar ratio of 7:1. | ProGP43 |
Linkage: β-GlcNAc-Asn.
N-linked hexasaccharide units - two residues each of Man and GlcNAc (one trisubstituted) and one residue each of Glc and 6-deoxy-6-sulfoglucose (6-sulfoquinovose, QuiS - a rare acidic sugar). β-D-Glcp-(1→4)-β-D-Quip(6--SO3-)-(1→3)-[α-D-Manp(1→4), α-D-Manp(1→6)] β-D-GlcpNAc-(1→4)-β-D-GlcpNAc-(1→Asn....
Microheterogeneity is observed as a family of glycans modify each of the glycosylation sites. The glycans are linked via a chitobiose core. | ProGP348 |
Linkage: β-GlcNAc-Asn.
Trisaccharide (779 Da) composed of β-Manp NAcA6Thr-(1– 4)- β-Glcp NAc3NAcA-(1–3)-β-Glcp NAc. The sugars are mannuronic acid with the attached threonine, diacetylated glucuronic acid, N-acetylglucosamine.
In a second version of this strain (M. voltae PS*), the glycan is modified with one additional residue (either 220 or 262 Da) linked to the terminal modified mannuronic acid. | ProGP239, ProGP240, ProGP241, ProGP242, ProGP243 |
| Linkage: β-GlcNAc-N-Asn. The major fractions consisted of “high mannose type” oligosaccharides containing 8–9 mannose residues. Bi- and tri-antennary “complex type” oligosaccharides having terminal galactose were detected as minor components. These oligosaccharides were N-linked and contained no sialic acid. A specific high-mannose type oligosaccharide linked to the MOMP of C. trachomatis mediates attachment and infectivity of the organism to HeLa cells. The lectin-binding properties of MOMP and its susceptibility to glycosidase indicate that MOMP glycoprotein contains α-Man and β-GlcNAc at the terminus. | ProGP77 |
| Linkage: β-GlcNAc-Thr/Ser. | ProGP233 |
Linkages: FucNAc-Ser.
Unusual homooligomers of α-1,5-linked D-arabinofuranose (α-1,5-D-Araf). Oligomer length varies from 3 to 8 units with an average length of 6 units. Trisaccharides of α-1,5-D-Araf are the principal modifications at Thr64 and Thr66, with additional mono- and disaccharides identified on Ser residues.
The pilin glycan is antigenically and chemically identical to that of Mycobacterium.
Microheterogeneity in glycosylation is observed. | ProGP231 |
| Linkages: Glc-N-Asn, Glc-O-Ser/Thr. Contains 9.4 ± 0.3% (w/w) sugar. | ProGP84, ProGP85 |
Linkages: Man- Ser.
The approx. weight of attached glycan was 1180 Da.
Branched heptasaccharide is: galactose-β(1–4)[galactose-α(1–3)](2-O-Me)fucose-β(1–4)xylose-β (1–4)glucuronic acid-α(1–2)[rhamnose-α(1–4)]mannose-α(1-O)Ser. | ProGP167 |
| Linkages: Man-N-Asn, Gal-O-Ser, Gal-O-Thr. | ProGP82, ProGP83 |
Linkages: Man-Ser.
The approx. weights of glycans attached to S328 and S455 were 1190Da and 1080 Da, respectively.
Branched heptasaccharide is: galactose-β(1–4)[galactose-α(1–3)](2-O-Me)fucose-β(1–4)xylose-β (1–4)glucuronic acid-α(1–2)[rhamnose-α(1–4)]mannose-α(1-O)Ser.
Only tetrasaccharide glycan observed in crystal structure: Man-(Rha)-GlcUA-Xyl (xylose-β (1–4)glucuronic acid-α(1–2)[rhamnose-α(1–4)]mannose-α(1-O)Ser. | ProGP132 |
| Linkages: ManNAc, GalNAc, BacNAc | ProGP64 |
Linkages: αMan-Thr/Ser.
Variable modifications with one to three hexose (mannose) units on each site. The total number of hexose units present is in the range of 6-10. The most dominant SodC glycoform was modified with nine hexose units. | ProGP190 |
Linkages: β-D-FucNAc-Ser.
A 666.5 Da trisaccharide containing pseudaminic acid, xylose, and N-acetylfucosamine [α-5NβOHC4 7NFmPse-(2→4)-β-Xyl-(1→3)-β-D-FucNAc-(1→3)-β-Ser].
Protein glycan molar ratio is 1:1.
It is structurally identical to the O-antigen repeating unit of P. aeruginosa serotype O7 LPS. This fact together with mutation studies suggest that the metabolic source of the pilin glycan is the O-antigen biosynthetic pathway. The pilin glycan differs from the O-antigen only in that the FucNAc residue is not O-acetylated. | ProGP107 |
| Linkages: β-GalNAc-N-Asn, β-Glc-N-Asn, Gal-O-Thr. | ProGP187 |
| Linkages: β-Glc-O-Tyr, β-Gal-O-Tyr. Glucosyl tyrosine is found in short-chain glycans while galactosyl tyrosine in long-chain glycans. It is the first report where the unusual monosaccharide 3-acetamido-3,6-dideoxy-D-glucose (quinovosamine) has been found to be present in S-layer glycoproteins. Repeating hexasaccharide [→4)[β-D-Quip3NAc-(1→6)-β-D-Galf-(1→4)-α-L-Rhap-(1→3)]-α-D-Manp-(1→4)-β-D-Galp-(→4)-β-D-Glcp-(1→]n=17 is present. Microheterogeneity is also observed. | ProGP111 |
Linkages: β-GlcNAc-Ser, HexNAc-Cys.
N-Acetylglucosamine is β-O-linked to Ser18 and N-acetylhexosamine is S-linked to C-terminal Cys43. | ProGP406 |
Linkages: βGalNAc- Asn, Glc-Asn, Gal-Thr.
Total carbohydrate content is approximately 10 to 12% of 200kDa S layer glycoprotein.
N glycosylated (at position N2) with GlcNAc-linked repeating (10-15 repeats) sulfated pentasaccharide and with sulphated oligosaccharides: (GlcA(OSO3-)-[β1→4GlcA(OSO3-)]2-β1→4Glc-Asn) at ten other poistions.
Abut 20 neutral di/ tri- saccharides α-D-Glc-(1→2)-Gal-(1→ or (uronic acid, glucose)-galactose are O-glycosidically attached to clustered threonine residues (14) adjacent to the TM domain at the C terminus but precise position of O glycosylated residues is not known. | ProGP3 |
| Man-Thr linkage | ProGP2 |
| Mannose, glucose, and an unknown sugar residue. | ProGP255 |
| Monosaccharide ( GlcNAc, Gal, Man) | ProGP462 |
| Monosaccharide ( mannose) | ProGP463, ProGP464, ProGP465, ProGP466, ProGP467, ProGP468, ProGP469, ProGP470, ProGP471 |
| Monosaccharide (Double HexNAc, HexNAc) | ProGP440, ProGP442, ProGP443, ProGP444, ProGP445, ProGP446, ProGP447, ProGP448, ProGP449, ProGP450, ProGP451 |
| Monosaccharide (rhamnose) | ProGP498, ProGP523 |
| Monosaccharide composition (by HPAEC): glucose, rhamnose, mannosamine, glucosamine, galactose and fucose. | ProGP169 |
| Monosaccharide composition detected by gas-liquid chromatography revealed the presence of several different isomers of glucose, galactose, N-acetylglucosamine, N-acetylgalactosamine, and rhamnose at a ratio of 29:5:39:1:1. | ProGP210 |
| More Pse5Ac7Ac (5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-L-manno-nonulosonic acid) in this case than from 81-176. Acetamidino and N-methylacetimidoyl derivatives of legionaminic acid, 315 Da Leg5Am7Ac (5-acetamidino-7-acetamido-3,5,7,9,-tetradeoxy-D-glycero-D-galacto-nonulosonic acid) and Leg5AmNMe7Ac (5-E/Z-N-(N-methylacetimidoyl)-7-acetamidino-3,5,7,9-tetradeoxy-D-glycero-D-galacto-nonulosonic), are also present. Leg5Ac7Ac (Leg) is legionaminic acid and Pse5Ac7Ac (Pse) is pseudaminic acid. | ProGP50 |
| Multiple heptose residues. | ProGP250 |
| N-acetylhexosaminyl residues | ProGP544, ProGP545 |
| N-glycan was identified as a branched trisaccharide containing a 225 Da residue which corresponds to a 2-amino-6-sulfo-2, 6-dideoxy-quinovose. Glcα-1,4-Gal disaccharide was identified as the O-glycan. Acidic trisaccharides linked to N306. | ProGP520 |
| N-glycan was identified as a branched trisaccharide containing a 225 Da residue which corresponds to a 2-amino-6-sulfo-2, 6-dideoxy-quinovose. Glcα-1,4-Gal disaccharide was identified as the O-glycan. Acidic trisaccharides linked to N307. | ProGP521 |
| O-AcHexDATDH (432 Da) and DATDH (229 Da) glycans. DATDH is 2,4 diacetamido-2,4,6 trideoxyhexose. | ProGP300 |
| O-AcHexDATDH (433 Da) and DATDH (229 Da) glycans. DATDH is 2,4 diacetamido-2,4,6 trideoxyhexose. | ProGP310 |
| O-AcHexDATDH (433 Da). DATDH is 2,4 diacetamido-2,4,6 trideoxyhexose. | ProGP299 |
| O-Linkage Ser/Thr. Glycosylated with structurally related monosaccharides of N-acetylpseudaminic acid (Pse5Ac7Ac8Ac). | ProGP248, ProGP249 |
| One covalently bound glucose molecule, per pilin subunit. | ProGP4 |
| Pentasaccharide (HexNAc-(Pen)-dHex3) | ProGP524, ProGP525, ProGP526, ProGP527, ProGP528, ProGP529, ProGP530, ProGP531 |
| Pentasaccharide (HexNAc-Hex-Hex-HexNAc-HexNAc) is linked through its terminal HexNAc residue to a 162.111-Da moiety via a phosphate bridge. | ProGP347 |
| Pentasaccharide (HexNAc-Hex-Hex-HexNAc-HexNAc). Orbitrap XL mass spectrometer using the higher-energy collisional fragmentation (HCD) mode suggests that pentasaccharide is linked through its terminal HexNAc residue to a 162.111-Da moiety via a phosphate bridge. | ProGP251 |
| Pentasaccharide composed of HexNAc-(Pen)-dHex3, which is similar to the O antigen subunit characterized in the LPS structures of multiple R. solanacearum strains. | ProGP532, ProGP533, ProGP534, ProGP535, ProGP536, ProGP537, ProGP538, ProGP539, ProGP540, ProGP541, ProGP542, ProGP543 |
| Pentasaccharide β-GlcNAc3NAcA4OAc-4-(β-GlcNAc-6-)-α-Gal-6-β-Glc-3-β-GalNAc-S/T. β-GlcNAc3NAcA4OAcA is an O-acetylated derivative of glucuronic acid. | ProGP416, ProGP417, ProGP418, ProGP419, ProGP420, ProGP421, ProGP422 |
| Presence of α-D-mannosyl linkage. | ProGP110 |
| Protein is associated with carbohydrate, and the carbohydrate is composed of sugars associated with the serotype b O-PS. The A. actinomycetemcomitans serotype b O-PS is a trisaccharide-repeating unit composed of D-Fuc, L-Rha, and D-GalNAc residues.Rha and Fuc were the main sugars identified in the analysis of the LPS isolated from the serotype b. | ProGP332 |
| Pseudaminic acid (Pse5Ac7Ac, 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-L-manno-nonulosonic acid). A total of seven O-linked Pse5Ac7Ac residues are present in FlaA protein. | ProGP216 |
| Pseudaminic acid (Pse5Ac7Ac, 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-L-manno-nonulosonic acid). O-linkage Ser/Thr at 10 sites. | ProGP215 |
| Recombinant G-enzyme (expressed in E. coli) shows GalNAc, Xyl, GlcNAc, Man, Glc, and Gal to account for 1.7%, 14.4%, 6.1%, 3.2%, 54.2%, and 10.4%, respectively, of total monosaccharides. G-enzyme from strain K-1 contains GalNAc, Xyl, GlcNAc, Man, Fuc, and Gal that account for 1.8%, 0.9%, 11.0%, 6.1%, 1.1%, and 79.1%, respectively, of total monosaccharides. | ProGP149 |
| Rha, Man, Gal, Glc, GalN(Ac), and GlcN(Ac) (i.e.,lacking Ara, Fuc, and NANA) totalling 2.1% by weight of protein. | ProGP178 |
| Rha-Asn linkage. Carbohydrates (7%), mainly rhamnose, ribose, and fucose. Rhamnose dominant. Glycan chains (15-30 residues) with branching points on rhamnosyl and ribosyl residues. | ProGP69 |
| Same sugar pattern like that of the wild-type is present. Galacturonic acid, 3-O-methyl-galacturonic acid, galactose, N-acetylglucosamine, and N-acetylgalactosamine have been observed. | ProGP166 |
| Several novel glycan moieties, ranging in mass from 494 Da to 917 Da. HexNAc and methylated deoxyhexose found. | ProGP324, ProGP325, ProGP326, ProGP327, ProGP328, ProGP329, ProGP330 |
| Single hexose at all each site. | ProGP516 |
| Six to eight Pse5Ac7Ac (pseudaminic acid) residues found in S/T-rich domain spanning residues 146 to 232. | ProGP297 |
| Six to eight Pse5Ac7Ac (pseudaminic acid) residues found in S/T-rich domain spanning residues 159 to 232. | ProGP296 |
Sugars identified include glucose, rhamnose, ribose, and glucosamine (amino sugar). 3.8% total amino sugar content determined.
In Bacillus cereus strain T, carbohydrates were determined using differential anthrone method, orcinol method, and aniline phthalic acid and ammoniacal silver nitrate reactions after descending paper chromatography. | ProGP168 |
| Sulfated oligoasccharide and heterosaccharides. It is the heterosaccharide moiety which is involved in keeping the rod form of halobacteria. | ProGP10 |
| Sulfoquinovose-hexose-based glycan, N-linked via a chitobiose core. In high salt (3.4 M NaCl) conditions, N13 and N83 are modified by a pentasaccharide while dolicholphosphate is modified by a tetrasaccharide comprising the first four pentasaccharide residues. Cells grown at low salinity (1.75 M NaCl) possess dolichol phosphate and N498 residue (in S-layer glycoprotein) modified by a distinct tetrasaccharide comprising a sulfated hexose, two hexoses and a rhamnose. | ProGP71 |
| Terminal α1,3-linked GalNAc. | ProGP232 |
| Tetrasaccharide containing (HexNAc)2, Hexose and N-acetyl-deoxyHexose. | ProGP499 |
| Tetrasaccharide, composed of two HexNAc residues, a Hexose and N-acetyl-deoxyHexose. | ProGP423 |
| The glycan identified was a T-antigen, Gal(β1–3)-GalNAc(β1–4)-GalNAc(β1–4)-GalNAcα1-Thr268. | ProGP452 |
| The minor fimbria contain the DC-SIGN-targeting carbohydrates fucose (1.35 nmol/mg), mannose (2.68 nmol/mg), N-acetylglucosamine (2.27 nmol/mg), and N-acetylgalactosamine (0.652 nmol/mg). | ProGP349 |
| The protein is extensively O-glycosylated with a 715-Da tetrasaccharide and a 324-Da disaccharide. The tetrasaccharide contains three rhamnose residues and an unusual terminal sugar, 2-O-methyl-4-
(3-hydroxy-3-methylbutanamido)-4,6-dideoxy-D-glucopyranose, named anthrose (Ant). The tetrasaccharide has the structure: β-Ant-(1→3)-α-L-Rhap-(1→3)-α-L-Rhap-(1→2)-L-Rhap. Oligosaccharide is attached to the BclA through a GalNAc linker. In its absence, GlcNAc can serve as a substitute linker. | ProGP217 |
| The relative molar content of monosaccharides: N-acetylglucosamine 1.0, mannose 4.9, galactose 2.1, glucose 5.5. The relative content of mannose and N-acetylglucosamine indicates the presence of oligomannose or hybrid-type oligosaccharides and the presence of galactose possibly indicates either O-linked or hybrid-types oligosaccharides. Addition of an N-linked oligosaccharide corresponding to an increase in apparent molecular mass of 3 kDa. | ProGP74 |
| The sites are glycosylated by an approximately 540 Da glycan that may be a derivative of the glycan of P. syringae pv. tabaci. | ProGP199 |
| Trihexose (mannose). | ProGP309 |
| Trisaccharide (NAG,NAA,any epimer of NAG); AllNacGlcNAc-Asn linkage; NAA is (epimer of NAG) N-acetylallosamine. | ProGP256 |
| Trisaccharide HexNAc-HexNAc-Hex. | ProGP472, ProGP473, ProGP474, ProGP475, ProGP476, ProGP477, ProGP478, ProGP479, ProGP480, ProGP481, ProGP482, ProGP483, ProGP484, ProGP485, ProGP486, ProGP487, ProGP488, ProGP489, ProGP490, ProGP491, ProGP492, ProGP493, ProGP494, ProGP495, ProGP496 |
| Two novel glycans - dimethylglyceric acid derivatives of pseudaminic acid (Pse5Ac7Ac) and 7-acetamidino pseudaminic acid are found. Ser181 and Ser207 were glycosylated by both glycans. Thr464 or Thr465 (or both, but exclusively) have been shown to be glycosylated by first glycan. Pse5Ac7Ac is also written as Pse5NAc7NAc (Pse). | ProGP206 |
| UDP-Glc, UDP-GlcNAc, UDP-Gal, GDP-Man and UDP-Xyl can serve as substrates for SunS GTase but UDP-α-D-glucose is most efficiently used. | ProGP404 |
| Unusual carbohydrate modification includes glucose, galactose, and possibly mannose and corresponds to 7–8 kDa of the molecular mass. 31 modification sites carry 47 hexose units indicating the presence of hexose and dihexose (162-Da) sugars. HMW1C is capable of transferring glucose and galactose to HMW1 and is also able to generate hexose-hexose bonds. | ProGP228 |
| β-D-Galp-(1→O)-Tyr glycosidic linkage unit. The glycan chains consist on average of twenty branched trisaccharide repeating units with the structure [→3)-β-D-Galp-(1→4)-[α-D-Glcp-(1→6)-]-β-D-ManpNAc-(1→]n. This O-antigen-like domain of the polysaccharide is connected with the S-layer polypeptide through the ‘‘core’’ structure →3)[GroA-(2→O)-PO2-(O→4)-β-D-ManpNAc-(1→4)]-α-L-Rhap-(1→3)-α-L-Rhap-(1→3)-α-L-Rhap-(1→3)-β-D-Galp-(1→O)-Tyr. Except for the substitution in position 4 of the nonreducing rhamnose with the modified glyceric acid phosphate residue GroA-2→OPO2→4-β-D-ManpNAc-(1→, this core is identical to the core of the Tyr-linked glycan from the S-layer glycoprotein of Tb. thermohydrosulfuricus L111-69. | ProGP75 |
| β-D-Glcp-(1→O)-L-Tyr linkage. Glycan chains with the repeating unit structure →4)[α-D-Galp-(1→2)]-α-L-Rhap-(1→3)[β-D-Glcp-(1→6)]-β-D-Manp-(1→4)-α-L-Rhap-(1→3)-α-D-Glcp-(1→. This is the first
observation of a bacterial S-layer glycan without a core region. | ProGP68 |
