ProGP111

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ProGP ID ProGP111
Validation Status Uncharacterized
Organism Information
Organism NameThermoanaerobacterium (formerly Clostridium) thermosaccharolyticum E207-71
Domain Bacteria
Classification Family: Thermoanaerobacteraceae
Order: Thermoanaerobacterales
Class: Clostridia
Division or phylum: "Firmicutes"
Protein Information
Protein NameS-layer glycoprotein
Subcellular LocationSurface
Function Structural component of the surface layer.
Glycosylation Status
Glycosylation Type O(Tyr)- linked
Glycosite Sequence Logo
Technique(s) used for Glycosylation DetectionChemical deglycosylation with TFMS
Glycan Information
Glycan Annotation Linkages: β-Glc-O-Tyr, β-Gal-O-Tyr. Glucosyl tyrosine is found in short-chain glycans while galactosyl tyrosine in long-chain glycans. It is the first report where the unusual monosaccharide 3-acetamido-3,6-dideoxy-D-glucose (quinovosamine) has been found to be present in S-layer glycoproteins. Repeating hexasaccharide [→4)[β-D-Quip3NAc-(1→6)-β-D-Galf-(1→4)-α-L-Rhap-(1→3)]-α-D-Manp-(1→4)-β-D-Galp-(→4)-β-D-Glcp-(1→]n=17 is present. Microheterogeneity is also observed.
BCSDB ID23144
Literature
Additional Comment100-210 kDa ladder-like bands represented glycosylated S-layer subunits.
Year of Identification1995
Year of Identification Month Wise1995.03
ReferenceAltman, E., Schaffer, C., Brisson, J.R. and Messner, P. (1996) Isolation and characterization of an amino sugar-rich glycopeptide from the surface layer glycoprotein of Thermoanaerobacterium thermosaccharolyticum E207-71. Carbohydr Res, 295, 245-253. [PubMed: 9002194]
AuthorAltman, E., Schaffer, C., Brisson, J.R. Messner, P.
Research GroupInstitute for Biological Sciences, National Research Council of Canada, Ottawa, Ont., Canada
Corresponding Author Messner, P.
ContactInstitute for Biological Sciences, National Research Council of Canada, Ottawa, Ont., Canada
ReferenceAltman, E., Schaffer, C., Brisson, J.R. and Messner, P. (1995) Characterization of the glycan structure of a major glycopeptide from the surface layer glycoprotein of Clostridium thermosaccharolyticum E207-71. Eur J Biochem, 229, 308-315.
AuthorAltman, E., Schaffer, C., Brisson, J.R. Messner, P.
Research GroupDivision of Biological Sciences, National Research Council, Ottawa, Canada.
Corresponding Author Messner, P.
ContactDivision of Biological Sciences, National Research Council, Ottawa, Canada.
Reference1) Messner, P., Steiner, K., Zarschler, K. and Schaffer, C. (2008) S-layer nanoglycobiology of bacteria. Carbohydr Res, 343, 1934-1951. [PubMed: 18336801]
Author Messner, P., Steiner, K., Zarschler, K. Schaffer, C.
Research GroupUniversität für Bodenkultur Wien, Zentrum für NanoBiotechnologie A-1180 Wien, Gregor-Mendel-Strasse 33, Austria. 
Corresponding Author Schaffer, C.
ContactUniversität für Bodenkultur Wien, Zentrum für NanoBiotechnologie A-1180 Wien, Gregor-Mendel-Strasse 33, Austria.