ProGP229

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ProGP ID ProGP229
Validation Status Characterized
Organism Information
Organism NameFlavobacterium columnare (formerly Flexibacter columnaris) ATCC 43622
Domain Bacteria
Classification Family: Flavobacteriaceae
Order: "Flavobacteriales"
Class: Flavobacteria
Division or phylum: "Bacteroidetes"
Taxonomic ID (NCBI) 996
Genome Sequence(s)
Organism Additional Information Flavobacterium columnare is known all over the world as a pathogen of freshwater fish. It causes columnaris disease that is initiated as an infection of the body surface and gills leading to chronic, ulcerative condition. The disease often results in fish death.
Protein Information
Protein NameShort glycopeptides
UniProtKB Sequence Val-Ala-Val-Asp-Ser-Ala-Ala (tripeptides to heptapeptides have been found with a minimal common fragment of Asp-Ser-Ala)
Glycosylation Status
Glycosylation Type O (Ser) linked
Experimentally Validated Glycosite(s) in Full Length ProteinS5
Experimentally Validated Glycosite(s ) in Mature ProteinS5
Glycosite(s) Annotated Protein Sequence VAVDS*(5)AA
Sequence Around Glycosites (21 AA) VAVDAA
Glycosite Sequence Logo
Technique(s) used for Glycosylation DetectionNMR spectra
Technique(s) used for Glycosylated Residue(s) Detection ESI Q-TOF-MS (electrospray quadrupole time of flight mass spectrometry) and sequencing
Glycan Information
Glycan Annotation Linkage: Man-Ser. The glycan is an unusual hexasaccharide composed of 4-O-methyl-2-acetamido-2-deoxy-D-glucuronic acid (GlcNAcA), D-glucuronic acid (D-GlcA), 2,3-di-O-acetyl-D-xylose (D-Xyl), 2-O-methyl-D-glucuronic acid (D-GlcA), D-mannose (D-Man), and 2-O-methyl-L-rhamnose (L-Rha).
BCSDB ID5522
Technique(s) used for Glycan Identification NMR spectra and monosaccharide analysis by GC of alditol acetates.
Literature
Additional CommentThe protein could not be identified as per the reference given. Only a heptapeptide was detected.
Year of Identification2003
Year of Identification Month Wise2003.11.14
Year of Validation 2003
Reference1) Vinogradov, E., Perry, M.B. and Kay, W.W. (2003) The structure of the glycopeptides from the fish pathogen Flavobacterium columnare. Carbohydr Res, 338, 2653-2658. [PubMed: 14670723]
Author Vinogradov, E., Perry, M.B. and Kay, W.W.
Research GroupInstitute for Biological Sciences, National Research Council, 100 Sussex Dr., Ottawa, ON, Canada K1A 0R6. evgeny.vinogradov@nrc-cnrc.gc.ca
Corresponding Author Kay, W.W.
ContactInstitute for Biological Sciences, National Research Council, 100 Sussex Dr., Ottawa, ON, Canada K1A 0R6. evgeny.vinogradov@nrc-cnrc.gc.ca