ProGP244

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ProGP ID ProGP244
Validation Status Characterized
Organism Information
Organism NameCampylobacter jejuni NCTC 11168
Domain Bacteria
Classification Family: Campylobacteraceae
Order: Campylobacterales
Class: Epsilonproteobacteria
Division or phylum: "Proteobacteria"
Taxonomic ID (NCBI) 197
Genome Sequence(s)
Organism Additional Information Campylobacter jejuni is a microaerophilic, Gram-negative, human pathogen that is the major cause of bacterial food-borne diarrhoea (gastroenteritis). It is most frequently responsible for a form of post-infection neuromuscular paralysis known as Guillain Barre' syndrome. It also leads to an immunoproliferative small intestine disease that is a rare malignant lymphoma of the intestine. Motility is essential for pathogenicity.
Protein Information
Protein NameSynthetic Glycopeptide
UniProtKB/SwissProt ID Not Applicable
UniProtKB Sequence KDFNVSKA
Sequence length 5 AA
Subcellular LocationNA
Function NA
Glycosylation Status
Glycosylation Type N (Asn) linked
Experimentally Validated Glycosite(s) in Full Length ProteinN4
Experimentally Validated Glycosite(s ) in Mature ProteinN4
Glycosite(s) Annotated Protein Sequence KDFN*(4)VSKA
Glycosite Sequence Logo
Technique(s) used for Glycosylation DetectionIn vitro chemo enzymatic glycosylation,HPLC analysis of glycopeptides with reference to known glycopeptide HPLC profiles
Technique(s) used for Glycosylated Residue(s) Detection Not applicable
Protein Glycosylation- Implication Not applicable
Glycan Information
Glycan Annotation GalNAc-α1,3-bacillosamine.
Protein Glycosylation linked (PGL) gene(s)
OST Gene NamePglB
Literature
Additional CommentEngineered glycopeptide.
In vitro characterization of the oligosaccharyl transferase activity of PglB protein from membrane fraction of Campylobacter jejuni using a synthetic disaccharide glycan donor (GalNAc-α1,3-bacillosamine-pyrophosphate-undecaprenyl) and a peptide acceptor substrate (KDFNVSKA).
Year of Identification2005
Year of Identification Month Wise2005.4.29
Year of Validation 2005
Reference1) Glover, K.J., Weerapana, E., Numao, S. and Imperiali, B. (2005) Chemoenzymatic synthesis of glycopeptides with PglB, a bacterial oligosaccharyl transferase from Campylobacter jejuni. Chem Biol, 12, 1311-1315. [PubMed: 16356848]
Author Glover, K.J., Weerapana, E., Numao, S. and Imperiali, B
Research GroupDepartment of Chemistry and Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
Corresponding Author Imperiali, B.
ContactDepartment of Chemistry and Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.