ProGP310

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ProGP ID ProGP310
Validation Status Characterized
Organism Information
Organism NameNeisseria gonorrhoeae (strain ATCC 700825 / FA 1090)
Domain Bacteria
Classification Family: Neisseriaceae
Order: Neisseriales
Class: Betaproteobacteria
Division or phylum: "Proteobacteria"
Taxonomic ID (NCBI) 242231
Genome Sequence(s)
GenBank AE004969.1
EMBL AE004969
Organism Additional Information Neisseria gonorrhoeae is the etiologic agent of the human disease gonorrhoea. It is equipped with a variety of adherence factors that help it in the colonization of diverse microenvironments in the human host. The property of phase and antigenic variation displayed by its type IV pilin enables it to avoid immune system thereby initiating the disease.
Gene Information
Gene NameNGO1043
NCBI Gene ID 3282593
GenBank Gene Sequence 3282593
Protein Information
Protein NameHypothetical protein
UniProtKB/SwissProt ID Q5F7X2
NCBI RefSeq YP_208127.1
EMBL-CDSAAW89715.1
UniProtKB Sequence >tr|Q5F7X2|Q5F7X2_NEIG1 Putative uncharacterized protein OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) GN=NGO1043 PE=4 SV=1 MKKLLIAAMMAAALAACSQEAKQEVKEAAQAVESDVKDTAASAAESAASAVEEAKGQVKD AAADAKASAEEAVTEAKDAAAETKEAVSEAAKDTLNKAADAAQEAADKMKDAAK
Sequence length 114 AA
Glycosylation Status
Glycosylation Type O (Ser) linked
Experimentally Validated Glycosite(s) in Full Length ProteinS88
Experimentally Validated Glycosite(s ) in Mature ProteinS88
Glycosite(s) Annotated Protein Sequence >tr|Q5F7X2|Q5F7X2_NEIG1 Putative uncharacterized protein OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) GN=NGO1043 PE=4 SV=1 MKKLLIAAMMAAALAACSQEAKQEVKEAAQAVESDVKDTAASAAESAASAVEEAKGQVKD AAADAKASAEEAVTEAKDAAAETKEAVS*(88)EAAKDTLNKAADAAQEAADKMKDAAK
Sequence Around Glycosites (21 AA) DAAAETKEAVSEAAKDTLNKA
Glycosite Sequence Logo
Technique(s) used for Glycosylation DetectionImmunoblotting with antiglycan antibodies
Technique(s) used for Glycosylated Residue(s) Detection The sole hydroxyl-bearing residue in the glycopeptide
Glycan Information
Glycan Annotation O-AcHexDATDH (433 Da) and DATDH (229 Da) glycans. DATDH is 2,4 diacetamido-2,4,6 trideoxyhexose.
Technique(s) used for Glycan Identification LC-ESI-MS/MS with CID (liquid chromatography-electrospray ionization–tandem mass spectrometry with collision-induced dissociation)
Protein Glycosylation linked (PGL) gene(s)
OST Gene NamePglO
OST ProGT IDProGT19
Literature
Additional CommentSequone feature: S/T plus low complexity region.
Year of Identification2009
Year of Identification Month Wise2009.4.17
Year of Validation 2009
Reference1) Vik, A., Aas, F.E., Anonsen, J.H., Bilsborough, S., Schneider, A., Egge-Jacobsen, W. and Koomey, M. (2009) Broad spectrum O-linked protein glycosylation in the human pathogen Neisseria gonorrhoeae. Proc Natl Acad Sci U S A, 106, 4447-4452. [PubMed: 19251655]
AuthorVik, A., Aas, F.E., Anonsen, J.H., Bilsborough, S., Schneider, A., Egge-Jacobsen, W. and Koomey, M.
Research GroupDepartment of Molecular Biosciences and Center for Molecular Biology and Neuroscience, University of Oslo, Oslo 0316, Norway.
Corresponding Author Koomey, M.
ContactDepartment of Molecular Biosciences and Center for Molecular Biology and Neuroscience, University of Oslo, Oslo 0316, Norway.