ProGP386

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ProGP ID ProGP386
Validation Status Characterized
Organism Information
Organism NameCampylobacter jejuni HB93-13
Domain Bacteria
Classification Family: Campylobacteraceae
Order: "Campylobacterales"
Class: "Epsilonproteobacteria"
Division or phylum: "Proteobacteria"
Taxonomic ID (NCBI) 192222
Genome Sequence(s)
GenBank NC_002163.1. 
EMBL AL111168 
Gene Information
Gene Namecj0843c
NCBI Gene ID 905140
GenBank Gene Sequence NC_002163.1. 
Protein Information
Protein NamePutative secreted transglycosylase
UniProtKB/SwissProt ID Q0PA47
NCBI RefSeq WP_002864855.1
EMBL-CDSCAL34971.1.
UniProtKB Sequence >tr|Q0PA47|Q0PA47_CAMJE Putative secreted transglycosylase OS=Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) GN=Cj0843c PE=4 SV=1 MIKKILVFLFIFSSLNATQYSIEKLKKEENSLAKDYYIYRLLEKNKISKKDAQDLNSHIF RYIGKIKSELEKIIPLKPYINPKYAKCYTYTANTILDANLTCQSVRLNSLVFIASLNSKD RTTLAQTFKNQRPDLTNLLLAFNTSDPMSYIVQKEDINGFFKLYNYSKKYDLDLNTGLVN KLPNHIGFKDFAQNIIIKKENPKFRYSMLEINPENVSEDSAFYLGVNALTYDKTELAYDF FKKAAQSFKSQSNKDNAIFWMWLIKNNEEDLKTLSQSSSLNIYSLYAKELTNTPFPKIES LNPSKKKNNFNMQDPFAWQKINKQIRDANASQLDVLAKEFDTQETLPIYAYILERKNNFK KHYFIMPYYDNIKDYNKTRQALILAIARQESRFIPTAISVSYALGMMQFMPFLANHIGEK ELKIPNFDQDFMFKPEIAYYFGNYHLNYLESRLKSPLFVAYAYNGGIGFTNRMLARNDMF KTGKFEPFLSMELVPYQESRIYGKKVLANYIVYRHLLNDSIKISDIFENLIQNKANDLNK S
Sequence length 541 AA
Glycosylation Status
Glycosylation Type N (Asn) linked
Experimentally Validated Glycosite(s) in Full Length ProteinN99, N175, N329 and N376
Glycosite(s) Annotated Protein Sequence >tr|Q0PA47|Q0PA47_CAMJE Putative secreted transglycosylase OS=Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) GN=Cj0843c PE=4 SV=1 MIKKILVFLFIFSSLNATQYSIEKLKKEENSLAKDYYIYRLLEKNKISKKDAQDLNSHIF RYIGKIKSELEKIIPLKPYINPKYAKCYTYTANTILDAN*(99)LTCQSVRLNSLVFIASLNSKD RTTLAQTFKNQRPDLTNLLLAFNTSDPMSYIVQKEDINGFFKLYNYSKKYDLDLN*(175)TGLVN KLPNHIGFKDFAQNIIIKKENPKFRYSMLEINPENVSEDSAFYLGVNALTYDKTELAYDF FKKAAQSFKSQSNKDNAIFWMWLIKNNEEDLKTLSQSSSLNIYSLYAKELTNTPFPKIES LNPSKKKNNFNMQDPFAWQKINKQIRDAN*(329)ASQLDVLAKEFDTQETLPIYAYILERKNNFK KHYFIMPYYDNIKDYN*(376)KTRQALILAIARQESRFIPTAISVSYALGMMQFMPFLANHIGEK ELKIPNFDQDFMFKPEIAYYFGNYHLNYLESRLKSPLFVAYAYNGGIGFTNRMLARNDMF KTGKFEPFLSMELVPYQESRIYGKKVLANYIVYRHLLNDSIKISDIFENLIQNKANDLNK S
Sequence Around Glycosites (21 AA) TYTANTILDANLTCQSVRLNS
NYSKKYDLDLNTGLVNKLPNH
QKINKQIRDANASQLDVLAKE
MPYYDNIKDYNKTRQALILAI
Glycosite Sequence Logo
Technique(s) used for Glycosylation DetectionSoybean Lectin Affinity Enrichment and ZIC-HILIC enrichment
Technique(s) used for Glycosylated Residue(s) Detection Reversed Phase LC-Tandem CID/HCD-MS and ESI (CID)-MS/MS after enrichment using graphite microcolumns
Glycan Information
Glycan Annotation Heptasaccharide GalNAc- α1,4-GalNAc- α1,4-(Glc β1,3)-GalNAc- α1,4-GalNAc- α1,4-GalNAc- α1,3-Bac- β1 where Bac is bacillosamine (2,4-diacetamido-2,4,6-trideoxyglucopyranose)
BCSDB ID20059
Technique(s) used for Glycan Identification Reversed Phase LC-Tandem CID/HCD-MS
Literature
Year of Identification2011
Year of Identification Month Wise2011.2.10
Year of Validation 2011
ReferenceScott NE, Parker BL, Connolly AM, Paulech J, Edwards AV, Crossett B, Falconer L, Kolarich D, Djordjevic SP, Højrup P, Packer NH, Larsen MR, Cordwell SJ. (2011) Simultaneous glycan-peptide characterization using hydrophilic interaction chromatography and parallel fragmentation by CID, higher energy collisional dissociation, and electron transfer dissociation MS applied to the N-linked glycoproteome of Campylobacter jejuni. Mol Cell Proteomics. 2011 Feb;10(2):M000031-MCP201.
AuthorScott NE, Parker BL, Connolly AM, Paulech J, Edwards AV, Crossett B, Falconer L, Kolarich D, Djordjevic SP, Højrup P, Packer NH, Larsen MR, Cordwell SJ
Research GroupSchool of Molecular and Microbial Biosciences, University of Sydney, Sydney, Australia
Corresponding Author Cordwell SJ
ContactSchool of Molecular and Microbial Biosciences, University of Sydney, Sydney, Australia