ProGP473

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ProGP ID ProGP473
Validation Status Characterized
Organism Information
Organism NameBurkholderia cenocepacia K56-2
Domain Bacteria
Classification Family: Burkholderiaceae
Order: Burkholderiales
Class: Betaproteobacteria
Division or phylum: "Proteobacteria"
Taxonomic ID (NCBI) 985075
Genome Sequence(s)
GenBank NZ_LAUA01000000
EMBL LAUA01000000
Organism Additional Information Causes opportunistic infections in plants, insects, animals, and humans
Gene Information
Gene Namebcal1081
GenBank Gene Sequence NC_011000.1. 
Protein Information
Protein NameMdtA multidrug resistance protein
UniProtKB/SwissProt ID B4ED17
NCBI RefSeq WP_006493330.1.
EMBL-CDSCAR51384.1
UniProtKB Sequence >tr|B4ED17|B4ED17_BURCJ Multidrug resistance protein MdtA OS=Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) GN=mdtA PE=3 SV=1 MDNEQKPTPPSKDPAPAAARRPRRTLLLGTVAVVVIGGLLWWHPWNRTPAAGSSAQGAGA SAAAGSHRGRGGPAAMANVPQPVQVATATQGEMPIVLSALGTVTPLANVTVKTQLSGYLQ SVSFQEGQIVKKGDVLAQIDPRPYQVALENAEGTHARDAALLATARLDLKRYQTLLSQDS IASQTVDTQASLVKQYEGAVKTDQAAIDSAKLNLTYARITAPVSGRVGLRQVDPGNYVTP GDTNGIVVITQLQPMSVIFTTSEDNLPQILKQVNAGRKLSVTAYNRNNTVPLETGSLATL DNQIDTSTGTVKLRANFDNKEGMLFPNQFVNTRLLVDVLRNATIVPTSAVLTGSIGQFVY VVKPDNTVTVRKVTIGPVDGERTSIVSGVMLGERVVTDGSDRLREGSKISIPADKPKGAS GAHGAGAASGASAASGAAGHRGGHRHGASQAAAPAAQ
Sequence length 457 AA
Subcellular LocationInner membrane
Function Membrane fusion protein spanning the periplasm. Component of RND (resistance, nodulation, division) transporters, involved in transmembrane transport.
Glycosylation Status
Glycosylation Type O (Ser/Thr) linked
Experimentally Validated Glycosite(s) in Full Length ProteinS449
Glycosite(s) Annotated Protein Sequence >tr|B4ED17|B4ED17_BURCJ Multidrug resistance protein MdtA OS=Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) GN=mdtA PE=3 SV=1 MDNEQKPTPPSKDPAPAAARRPRRTLLLGTVAVVVIGGLLWWHPWNRTPAAGSSAQGAGA SAAAGSHRGRGGPAAMANVPQPVQVATATQGEMPIVLSALGTVTPLANVTVKTQLSGYLQ SVSFQEGQIVKKGDVLAQIDPRPYQVALENAEGTHARDAALLATARLDLKRYQTLLSQDS IASQTVDTQASLVKQYEGAVKTDQAAIDSAKLNLTYARITAPVSGRVGLRQVDPGNYVTP GDTNGIVVITQLQPMSVIFTTSEDNLPQILKQVNAGRKLSVTAYNRNNTVPLETGSLATL DNQIDTSTGTVKLRANFDNKEGMLFPNQFVNTRLLVDVLRNATIVPTSAVLTGSIGQFVY VVKPDNTVTVRKVTIGPVDGERTSIVSGVMLGERVVTDGSDRLREGSKISIPADKPKGAS GAHGAGAASGASAASGAAGHRGGHRHGAS*(449)QAAAPAAQ
Glycosite Sequence Logo
Technique(s) used for Glycosylation DetectionZIC-HILIC,immunoblotting,tryptic digestion, and MS/MS analysis
Technique(s) used for Glycosylated Residue(s) Detection MS/MS analysis
Glycan Information
Glycan Annotation Trisaccharide HexNAc-HexNAc-Hex.
Protein Glycosylation linked (PGL) gene(s)
OST Gene NamePglLBC
OST ProGT IDProGT72
Literature
Year of Identification2014
Year of Identification Month Wise2014.3.5
ReferenceLithgow KV, Scott NE, Iwashkiw JA, Thomson EL, Foster LJ, Feldman MF, Dennis JJ. (2014) A general protein O-glycosylation system within the Burkholderia cepacia complex is involved in motility and virulence. Mol Microbiol., 92(1):116-37.
AuthorLithgow KV1, Scott NE, Iwashkiw JA, Thomson EL, Foster LJ, Feldman MF, Dennis JJ.
Research GroupDepartment of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada,
Corresponding Author Dennis JJ.
ContactDepartment of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada,