ProGP496 (PliA)

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ProGP ID ProGP496 (PliA)
Validation Status Characterized
Organism Information
Organism NameAcinetobacter nosocomialis
Domain Bacteria
Classification Family: Moraxellaceae
Order: Pseudomonadales
Class: Gammaproteobacteria
Division or phylum: "Proteobacteria"
Taxonomic ID (NCBI) 1217985
Genome Information
GenBank NZ_KB849570.1.
EMBL APPP01000010
Organism Additional Information Acinetobacter nosocomialis is a medically relevant strain causing bacteremic nosocomial pneumonia.
Protein Information
Protein NamePliA
Glycosylation Status
Glycosylation Type O- (Ser) linked
Experimentally Validated Glycosite(s) in Full Length ProteinS136
Technique(s) used for Glycosylation DetectionWestern blot analysis
Technique(s) used for Glycosylated Residue(s) Detection Western blot analysis of whole cell extracts probing for PilAM2 expression and electrophoretic mobility on S132A and S136A mutant, MS/MS analysis
Glycan Information
Glycan Annotation Tetrasaccharide containing (HexNAc)2, Hexose and N-acetyl-deoxyHexose.
Protein Glycosylation linked (PGL) gene(s)
OST ProGT IDProGT85 (TfpOM2)
Literature
ReferenceHarding CM, Nasr MA, Kinsella RL, Scott NE, Foster LJ, Weber BS, Fiester SE, Actis LA, Tracy EN, Munson RS Jr, Feldman MF. (2015) . Acinetobacter strains carry two functional oligoligoligosaccharyltransferases, one devoted exclusively to type IV pilin, and the other one dedicated to O-glycosylation of multiple proteins. Mol Microbiol. 5, 1023-41. [PMID: 25727908]
AuthorHarding CM, Nasr MA, Kinsella RL, Scott NE, Foster LJ, Weber BS, Fiester SE, Actis LA, Tracy EN, Munson RS Jr, Feldman MF.
Research Group1 Center for Microbial Pathogenesis, The Research Institute at Nationwide Childrens Hospital, Columbus, OH, USA. 2 Department of Pediatrics, College of Medicine, The Ohio State University, Columbus, OH, USA. 3 Biomedical Sciences Graduate Program, College of Medicine, The Ohio State University, Columbus, OH, USA. 4 Department of Biological Sciences, University of Alberta, Edmonton, AB, T6G 2G2, Canada. 5 Centre for High-Throughput Biology, University of British Columbia, Vancouver, BC, Canada. 6 Department of Microbiology, Miami University, Oxford, OH, USA
Corresponding Author Feldman MF.
ContactDepartment of Biological Sciences, University of Alberta, Edmonton, AB, T6G 2G2, Canada.