ProGP528

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ProGP ID ProGP528
Validation Status Characterized
Organism Information
Organism NameRalstonia solanacearum GMI1000
Domain Bacteria
Classification Family:Burkholderiaceae
Order: Burkholderiales
Class: Betaproteobacteria
Division or phylum: "Proteobacteria"
Taxonomic ID (NCBI) 267608
Genome Sequence(s)
GenBank NC_003295.1
EMBL AL646052.1
Gene Information
Gene NameragB
NCBI Gene ID 1221120
GenBank Gene Sequence NC_003295.1
Protein Information
Protein NameRagB
UniProtKB/SwissProt ID Q8XX43
NCBI RefSeq WP_011002201.1
EMBL-CDSCAD15982.1
UniProtKB Sequence >tr|Q8XX43|Q8XX43_RALSO Probable polysaccharide transport system component signal peptide protein OS=Ralstonia solanacearum (strain GMI1000) GN=ragB PE=4 SV=1 MWSADLAPRERLLPPWLIGTLGGFVALALAMMFPRERLETRLLEGGKVDALSMAYLEAWL RVRPNDGEFLAVLAAQYVRTGRLDEAEAMLARMRTLHDKAIGREALLLDIAIREQRAYAL QPGDARRKAYLDELRGLLGQALDYRWTASEMEVLAGQARGLDAGAIAARFYQRLSKQDPS RAAQWQSRSAEIALGVGDYRAAAAANFAAQAAASSLEERRRYFMGGLKALQAGGLLDEAM REAQQRAGPLIDDQETLRFLTRLAMACNRADLADYYARRLLHLSWQAAPDAAIALAEGAA RHDWVFGGRPQPLPSPSEAVFLDGVEGVARRPRGAARIRRVADAASAPAAPPAGAQAAAH GIAPFNAEDYDLAFRVFLGSGNLNDAMRVAEAAVRQRPDLKLWTERAAQVAEWNHQPMVA LQHWLAYAQATGDDVAWRNVLRLAPALNEDHAYLAALRHKAGSQPADMKLLDQIVAAYER LGEPAEGLAYLDSVARGPNRQAVLERYAQLAERAGKDDLAYAGYQRLQREFGPHAGYALK LANMLYVRDQFELALAAMQTAEHIASPTDDLYWRTFAQLARLNQRDDLMREAYRQLLIGG GAVVDDLSTMVEFYDASPIDAGRLAELAYRKDGGPIHLQRALYYYTRAGAFHRVEGLLAS LTPAQRAAAERSAGVLAARAEYYRQRGQWDAAMRDLRCAVSLPDAGSDVKAALLWSLIDS GQIAELKRALAHWRGEAENDSVYWGAYAAAGLRLADPGLALRFLGRQGKSMRNDPLWLLA YADAREMAGQADVAWRLRREAWRLLWRGEEAGAGAPGERRAAASDEVQPAALEDGARAEL RARRATLAQTFGSGDLAQRLLIELLRDDRRETRAAREGGRAAIAGSELGDLDVLPPEPPE SPAASRNAARASDQLVSAIAKEAALGWALSQEANDLARAWLLQQYARGAARPAYAEISIA LAERDLPTLNRLLDDTPDRLPLAARIDANAMTDRLGEAQRLAFGGLTTAPDEPQLHQRVE ETLLANAQALEPRGTWYKQAPLTYFESSAAAGTRLTDHYSMLLRATQRNQRSIDAQQLTG VPAQDRSVDWISQYLTRDARWKGTVGWRQGLASFTTFRLDGQLGQTGPVVTEFAVGRNQP ASETPQLRVGGVKDIATIGANWRFTQRQYVRARVEGDRFYGQDRSFLGSGTVFDGEIGHR FRVEYPDYTIRLTGTYARYSASGAPGAPLGRLLPAGAAMSAEQFVPRSFAQYGLLFGFGT DYLERYARAWRPFLDAGMLHDNRQGWGVQLQVGAAGSVLGNDHLALYIGHASITRAGTSP MTEIGLRYRWLY
Sequence length 1332 AA
Function Probable polysaccharide transport system component
Glycosylation Status
Glycosylation Type O- (Ser/Thr) linked
Experimentally Validated Glycosite(s) in Full Length ProteinS346
Glycosite(s) Annotated Protein Sequence >tr|Q8XX43|Q8XX43_RALSO Probable polysaccharide transport system component signal peptide protein OS=Ralstonia solanacearum (strain GMI1000) GN=ragB PE=4 SV=1 MWSADLAPRERLLPPWLIGTLGGFVALALAMMFPRERLETRLLEGGKVDALSMAYLEAWL RVRPNDGEFLAVLAAQYVRTGRLDEAEAMLARMRTLHDKAIGREALLLDIAIREQRAYAL QPGDARRKAYLDELRGLLGQALDYRWTASEMEVLAGQARGLDAGAIAARFYQRLSKQDPS RAAQWQSRSAEIALGVGDYRAAAAANFAAQAAASSLEERRRYFMGGLKALQAGGLLDEAM REAQQRAGPLIDDQETLRFLTRLAMACNRADLADYYARRLLHLSWQAAPDAAIALAEGAA RHDWVFGGRPQPLPSPSEAVFLDGVEGVARRPRGAARIRRVADAASAPAAPPAGAQAAAH GIAPFNAEDYDLAFRVFLGS*(346)GNLNDAMRVAEAAVRQRPDLKLWTERAAQVAEWNHQPMVA LQHWLAYAQATGDDVAWRNVLRLAPALNEDHAYLAALRHKAGSQPADMKLLDQIVAAYER LGEPAEGLAYLDSVARGPNRQAVLERYAQLAERAGKDDLAYAGYQRLQREFGPHAGYALK LANMLYVRDQFELALAAMQTAEHIASPTDDLYWRTFAQLARLNQRDDLMREAYRQLLIGG GAVVDDLSTMVEFYDASPIDAGRLAELAYRKDGGPIHLQRALYYYTRAGAFHRVEGLLAS LTPAQRAAAERSAGVLAARAEYYRQRGQWDAAMRDLRCAVSLPDAGSDVKAALLWSLIDS GQIAELKRALAHWRGEAENDSVYWGAYAAAGLRLADPGLALRFLGRQGKSMRNDPLWLLA YADAREMAGQADVAWRLRREAWRLLWRGEEAGAGAPGERRAAASDEVQPAALEDGARAEL RARRATLAQTFGSGDLAQRLLIELLRDDRRETRAAREGGRAAIAGSELGDLDVLPPEPPE SPAASRNAARASDQLVSAIAKEAALGWALSQEANDLARAWLLQQYARGAARPAYAEISIA LAERDLPTLNRLLDDTPDRLPLAARIDANAMTDRLGEAQRLAFGGLTTAPDEPQLHQRVE ETLLANAQALEPRGTWYKQAPLTYFESSAAAGTRLTDHYSMLLRATQRNQRSIDAQQLTG VPAQDRSVDWISQYLTRDARWKGTVGWRQGLASFTTFRLDGQLGQTGPVVTEFAVGRNQP ASETPQLRVGGVKDIATIGANWRFTQRQYVRARVEGDRFYGQDRSFLGSGTVFDGEIGHR FRVEYPDYTIRLTGTYARYSASGAPGAPLGRLLPAGAAMSAEQFVPRSFAQYGLLFGFGT DYLERYARAWRPFLDAGMLHDNRQGWGVQLQVGAAGSVLGNDHLALYIGHASITRAGTSP MTEIGLRYRWLY
Sequence Around Glycosites (21 AA) ARIRRVADAASAPAAPPAGAQ 
Technique(s) used for Glycosylation DetectionZIC-HILIC
Technique(s) used for Glycosylated Residue(s) Detection LC-MS, CID MS/MS and HCD MS/MS
Glycan Information
Glycan Annotation Pentasaccharide (HexNAc-(Pen)-dHex3)
Protein Glycosylation linked (PGL) gene(s)
OST Gene NamePglLRs
OST ProGT IDProGT103
Literature
Year of Identification2016
Year of Identification Month Wise2016.3.26
Year of Validation 2016
ReferenceElhenawy W, Scott NE, Tondo ML, Orellano EG, Foster LJ, Feldman MF. (2016) Protein O-linked glycosylation in the plant pathogen Ralstonia solanacearum. Glycobiology., 26(3):301-11. [PMID: 26531228]
AuthorElhenawy W, Scott NE, Tondo ML, Orellano EG, Foster LJ, Feldman MF.
Research Group1 Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada. 2 Centre for High-Throughput Biology, University of British Columbia, Vancouver, BC, Canada. 3 Faculty of Biochemical and Pharmaceutical Sciences (FBIOYF-UNR), Institute of Molecular and Cellular Biology of Rosario (IBR-CONICET), Rosario, Santa Fe, Argentina. 4 Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada Department of Molecular Microbiology, Washington University School of Medicine St. Louis, St. Louis, MO, USA
Corresponding Author  Feldman MF
ContactDepartment of Biological Sciences, University of Alberta, Edmonton, AB, Canada Department of Molecular Microbiology, Washington University School of Medicine St. Louis, St. Louis, MO, USA