Latest update: September 24, 2018


ProGT122 (AaNGT)

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ProGT ID ProGT122 (AaNGT)
Organism Information
Organism NameAggregatibacter aphrophilus
Clinical ImplicationPathogenic
DomainBacteria
PhylumProteobacteria
ClassificationFamily: Pasteurellaceae
Order: Pasteurellales
Class: Gammaproteobacteria
Phylum: Proteobacteria
Taxonomic ID (NCBI)732
Genome Information
Gene Information
Protein information
Protein NameAaNGT 
Additional Information1) AaNGT is the first identified natural glycosyltransferase able to transfer GlcN moiety onto asparagine residues.
2) AaNGT also exhibited a different position-specific residue preference of substrate peptides from the NGT of Actinobacillus pleuropneumoniae (ApNGT).
Glycosyltransferase Information
Glycosylation TypeN- (Asn) linked 
CAZY FamilyGT41
Mechanism of Glycan TransferSequential
Acceptor specificity Sequon_1Asn-Xaa-Ser/Thr
Donor TypeNucleotide activated sugars
Donor SpecificityUDP-Glc (Most preferred), UDP-Gal, UDP-Xyl, GDP-Glc, dGDP-Glc and UDP-GlcN
Glycan Information
Glycan transferredMonosaccharides (Glc, Gal, Xyl, and GlcN) 
Method of Glycan IndentificationLC/MS-IT-TOF
Experimental_strategiesIn vitro 
Acceptor Subtrate Information
Acceptor Substrate name TAMRA-DANYTK
Acceptor Substrate name TLDDNGTMLFFK (human Hemopexin)
Litrature
Year Of Validation2018 
Reference Kong Y, Li J, Hu X, Wang Y, Meng Q, Gu G, Wang PG, Chen M. N-Glycosyltransferase from Aggregatibacter aphrophilus synthesizes glycopeptides with relaxed nucleotide-activated sugar donor selectivity. Carbohydrate research. 2018 Jun 15;462:7-12.

Authors Kong Y, Li J, Hu X, Wang Y, Meng Q, Gu G, Wang PG, Chen M.
Research groups1 The State Key Laboratory of Microbial Technology, National Glycoengineering Research Center and School of Life Sciences, Shandong University, Jinan, Shandong 250100, China. 2 The State Key Laboratory of Microbial Technology, National Glycoengineering Research Center and School of Life Sciences, Shandong University, Jinan, Shandong 250100, China; Department of Chemistry, Georgia State University, Atlanta, GA 30303, United States. 3 The State Key Laboratory of Microbial Technology, National Glycoengineering Research Center and School of Life Sciences, Shandong University, Jinan, Shandong 250100, China.
Corresponding AuthorChen M.
Contacts1 The State Key Laboratory of Microbial Technology, National Glycoengineering Research Center and School of Life Sciences, Shandong University, Jinan, Shandong 250100, China.