Latest update: September 24, 2018


ProGT44 (NGT)

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ProGT ID ProGT44 (NGT)
Organism Information
Organism NameYersinia enterocolitica strain 8081
Clinical ImplicationPathogenic
DomainBacteria
PhylumProteobacteria
ClassificationFamily: Yersiniaceae
Order: Enterobacterales
Class: Gammaproteobacteria
Phylum: Proteobacteria
Taxonomic ID (NCBI)393305
Genome Information
Gene BankAM286415.1
EMBLAM286415.1
Gene Information
Protein information
Protein NameNGT 
Subcellular LocationCytoplasm
Glycosyltransferase Information
Glycosylation TypeN- (Asn) linked 
CAZY FamilyGT41
EC Number (BRENDA)2.4.1.-
Mechanism of Glycan TransferSequential
Acceptor specificity Sequon_1NX(S/T)
Donor TypeNucleotide activated sugars
Donor SpecificityUDP-Glc and UDP-Gal ( UDP-Glc is preferred over UDP-Gal)
Glycan Information
Glycan transferredMonosaccharides (Glc and Gal) 
Experimental_strategiesIn vitro 
Acceptor Subtrate Information
Acceptor Substrate name Hexapeptide DANYTK (labeled with TAMRA at the N terminus)
Litrature
Year Of Validation2011 
Reference Schwarz, F., Fan, Y. Y., Schubert, M., & Aebi, M. (2011). Cytoplasmic N-glycosyltransferase of Actinobacillus pleuropneumoniae is an inverting enzyme and recognizes the NXS/T consensus sequence. Journal of Biological Chemistry, jbc-M111.

Authors Schwarz, F., Fan, Y. Y., Schubert, M., & Aebi, M.
Research groupsMolecular Biology and Biophysics, Department of Biology, ETH Zürich, 8093 Zürich, Switzerland
Corresponding Author Aebi, M.
ContactsMolecular Biology and Biophysics, Department of Biology, ETH Zürich, 8093 Zürich, Switzerland Inst. of Microbiology, Dept. of Biology, ETH Zürich, Wolfgang-Pauli-Str. 10, 8093 Zürich, Switzerland