Latest update: September 24, 2018


ProGT67 (SdgB)

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ProGT ID ProGT67 (SdgB)
Organism Information
Organism NameStaphylococcus aureus
Clinical ImplicationPathogenic
DomainBacteria
PhylumFirmicutes
ClassificationFamily: Clostridiaceae
Order: Clostridiales
Class: Clostridia
Phylum: Firmicutes
Taxonomic ID (NCBI)367830
Genome Information
Gene BankCP000255
EMBLCP000255
Gene Information
Gene NameSAUSA300_0550
NCBI Reference SequenceABD21647.1
Protein information
Protein NameSdgB 
UniProtKB/ SwissProt IDA0A0H2XGN0
NCBI Ref SeqWP_001106113.1
UniProtKB Sequence>tr|A0A0H2XGN0|A0A0H2XGN0_STAA3 Glycosyl transferase, group 1 family protein OS=Staphylococcus aureus (strain USA300) GN=SAUSA300_0550 PE=4 SV=1 MNYFVGNSLGVNLTGIEKAIINRLNLFKEMGRPAQCVFLSWNRYLYRNAQNYITSSDYIN MYDFFQEATYLERNEPFDWLSYWTDECHYTLKHVENSHDFRIYDQERFLMYAHFQDPKYR ILDYVNHFDSQRRKVKRDFYDVRGFLSCSRILVDKQQTLCEFFYNPEGDTKLEKYFSYKD GKPEVQKIIVYYANKQYFFNNETELGAFFIKQLYQHGDLFFSDRNVYTAPIFNLTPESIP VVAVLHSTHIKNIDALDSSPFKNVYKAMFENLSRYRAIIVSTEQQKLDVEKRINHTIPVV NIPVGYSETIDTPVQTLDQRSVKLISVARYSPEKQLHQQIELIKRLVSYVPKIELHMYGF GSESKKLNELIQKYGLENHVYLRGFLSNLDQEYSDAYLSLITSNMEGFSLALLESLAHGV PVISYDIKYGPNELITSDFNGYLITKNDEDALFDKVKYVIDHPEVQQRLSKGSLAKAQQY SKASLIKQWDQFVRLI
EMBL CDSABD21647.1
Sequence length496 AA
Function in Native Organism 1) SdgB transfer the first GlcNAc to the SDR Protein.
Potential Application
Additional Information1) SdgB adds GlcNAc residue to the SDR protein.
2) SdgB creates an immunodominant epitope for highly opsonic human antibodies by transferring GlcNAc residue on SDR-proteins.
3) aggB (sdgA) and aggC (sdgB) are involved in GlcNAc modification of the ClfA SD repeats.
Glycosyltransferase Information
Glycosylation TypeO- (Ser/Thr) linked 
CAZY FamilyGT4
EC Number (BRENDA)2.4.1.109
Mechanism of Glycan TransferSequential
Acceptor specificity Sequon_1Serine-Aspartate dipeptide-Repeats (SDR)
Donor TypeNucleotide activated sugars
Donor SpecificityUDP-GlcNAc
Glycan Information
Glycan transferredMonosaccharide (GlcNAc) 
Method of Glycan IndentificationLC-MS
Experimental_strategiesIn vivo and In vitro 
Acceptor Subtrate Information
Acceptor Substrate name Pls (Plasmin sensitive surface protein)
ProGPdb ID ProGP551
Acceptor Substrate name ClfASD5
Litrature
Year Of Validation2013 
Reference Hazenbos, W.L., Kajihara, K.K., Vandlen, R., Morisaki, J.H., Lehar, S.M., Kwakkenbos, M.J., Beaumont, T., Bakker, A.Q., Phung, Q., Swem, L.R. & Ramakrishnan, S. (2013). Novel staphylococcal glycosyltransferases SdgA and SdgB mediate immunogenicity and protection of virulence-associated cell wall proteins. PLoS pathogens, 9(10), e1003653.

Authors Hazenbos, W.L., Kajihara, K.K., Vandlen, R., Morisaki, J.H., Lehar, S.M., Kwakkenbos, M.J., Beaumont, T., Bakker, A.Q., Phung, Q., Swem, L.R. & Ramakrishnan, S.
Research groupsDept. of Infectious Diseases, Genentech, Inc., San Francisco, California, United States of America.
Corresponding Author Ramakrishnan, S.
ContactsDept. of Infectious Diseases, Genentech, Inc., San Francisco, California, United States of America
Reference Thomer, L., Becker, S., Emolo, C., Quach, A., Kim, H.K., Rauch, S., Anderson, M., LeBlanc, J.F., Schneewind, O., Faull, K.F. & Missiakas, D. (2013). N-acetylglucosaminylation of serine-aspartate repeat proteins promotes Staphylococcus aureus blood stream infection. Journal of Biological Chemistry, jbc-M113.

Authors Thomer, L., Becker, S., Emolo, C., Quach, A., Kim, H.K., Rauch, S., Anderson, M., LeBlanc, J.F., Schneewind, O., Faull, K.F. & Missiakas, D.
Research groupsDepartment of Microbiology, The University of Chicago, Chicago, Illinois 60637
Corresponding Author Missiakas, D.
ContactsDepartment of Microbiology, The University of Chicago, Chicago, Illinois 60637