Latest update: September 24, 2018


ProGT76 (GtfB)

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ProGT ID ProGT76 (GtfB)
ProGT Pathway
Organism Information
Organism NameStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Clinical ImplicationPathogenic
DomainBacteria
PhylumFirmicutes
ClassificationFamily: Streptococcaceae
Order: Lactobacillales
Class: Bacilli (or Firmibacteria)
Division or phylum: "Firmicutes"
Taxonomic ID (NCBI)170187
Genome Information
Gene BankAE005672
EMBLAE005672
Gene Information
Gene NamegtfB
NCBI Reference SequenceNZ_AKVY01000001.1.
Protein information
Protein NameGtfB 
UniProtKB/ SwissProt IDA0A0H2UR90
NCBI Ref SeqWP_000571755.1
UniProtKB Sequence>tr|A0A0H2UR90|A0A0H2UR90_STRPN Glycosyltransferase stabilizing protein Gtf2 OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) GN=gtf2 PE=3 SV=1 MIELYDSYSQESRDLHESLGATGLSQLGVVIDADGFLPDGLLSPFTYYLGYEDGKPLYFN QVPVSDFWEILGDNQSACIEDVTQERAVIHYADGMQARLVKQVDWKDLEGRVRQVDHYNR FGACFATTTYSADSEPIMTVYQDVNGQQVLLENHVTGDILLTLPGQSMRYFANKVEFITF FLQDLEIDTSQLIFNTLATPFLVSFHHPDKSGSDVLVWQEPLYDAIPGNMQLILESDNVR TKKIIIPNKATYERALELTDEKYHDQFVHLGYHYQFKRDNFLRRDALILTNSDQIEQVEA IAGALPDVTFRIAAVTEMSSKLLDMLCYPNVALYQNASPQKIQELYQLSDIYLDINHSNE LLQAVRQAFEHNLLILGFNQTVHNRLYIAPDHLFESSEVAALVETIKLALSDVDQMRQAL GKQGQHANYVDLVRYQETMQTVLGG
EMBL CDSAAK75832.1
Sequence length445 AA
Subcellular LocationMembrane (Integral component of membrane)
Additional Information1) DUF1975, a domain of GtfA necessary for the formation of active GtfA-GtfB complex, the Beta-meander add-on domain DUF1975 of GtfA is critical for the recognition of both co-activator GtfB and acceptor PsrP.
2) GtfB functions as a co-activator/chaperone for the full activity of GtfA.
Glycosyltransferase Information
Glycosylation TypeO- (Ser/Thr) linked 
CAZY FamilyGT8
EC Number (BRENDA)2.4.1.-
Mechanism of Glycan TransferSequential
Donor TypeNucleotide activated sugars
Donor SpecificityUDP-GlcNAc
Accessory GT IDProGT75.1 ProGT75.2 ProGT75.3 ProGT75.4 ProGT75.5 ProGT75.6 ProGT75.7
Glycan Information
Glycan transferredMonosaccharide (GlcNAc) 
Method of Glycan IndentificationLTQ Orbitrap MS
Acceptor Subtrate Information
Acceptor Substrate name PsrP (pneumococcal serine-rich repeat protein)
ProGPdb ID ProGP449
Litrature
Year Of Validation2014 
Reference Shi, W.W., Jiang, Y.L., Zhu, F., Yang, Y.H., Shao, Q.Y., Yang, H.B., Ren, Y.M., Wu, H., Chen, Y. & Zhou, C. Z. (2014). Structure of a novel O-GlcNAc transferase GtfA reveals insights into the glycosylation of pneumococcal serine-rich repeat adhesins. Journal of Biological Chemistry, jbc-M114.

Authors Shi, W.W., Jiang, Y.L., Zhu, F., Yang, Y.H., Shao, Q.Y., Yang, H.B., Ren, Y.M., Wu, H., Chen, Y. & Zhou, C. Z.
Research groupsHefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China
Corresponding Author Zhou, C. Z.
ContactsHefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China.
Reference Jiang, Y. L., Jin, H., Yang, H. B., Zhao, R. L., Wang, S., Chen, Y., & Zhou, C. Z. (2017). Defining the enzymatic pathway for polymorphic O-glycosylation of the pneumococcal serine-rich repeat protein PsrP. The Journal of Biological Chemistry, 292, 6213-6224.

Authors Jiang, Y. L., Jin, H., Yang, H. B., Zhao, R. L., Wang, S., Chen, Y., & Zhou, C. Z.
Research groups1 Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China and. 2 Key Laboratory of Structural Biology, Chinese Academy of Science, Hefei, Anhui 230027, China. 3 Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China. 4 Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China.
Corresponding Author Zhou, C. Z.
Contacts1 Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China and. 2 Key Laboratory of Structural Biology, Chinese Academy of Science, Hefei, Anhui 230027, China. 3 Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China. 4 Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China.