Latest update: September 24, 2018


ProGT85 (TfpOM2)

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ProGT ID ProGT85 (TfpOM2)
Organism Information
Organism NameAcinetobacter nosocomialis strain M2
Clinical ImplicationPathogenic
DomainBacteria
PhylumProteobacteria
ClassificationFamily: Moraxellaceae
Order: Pseudomonadales
Class: Gammaproteobacteria
Phylum: Proteobacteria
Taxonomic ID (NCBI)1217985
Genome Information
Gene BankAPPP01000010
EMBLAPPP01000010
Gene Information
Gene NametfpO
Protein information
Protein NameTfpOM2 
Subcellular LocationMembrane (Integral component of membrane)
Additional Information1) TfpOM2 is required for the glycosylation of pilA at its terminal serine residue.
2) The carboxy-terminal serine 136 of PilAM2 is modified by glycosyltransferase TfpOM2.
Glycosyltransferase Information
Glycosylation TypeO- (Ser/Thr) linked 
EC Number (BRENDA)2.4.1.-
Mechanism of Glycan TransferEn Bloc
Donor TypeLipid linked sugars
Donor SpecificityUndPP-Tetrasaccharide
Glycan Information
Glycan transferredTetrasaccharide containing (HexNAc)2, Monosaccharide ( hexose) and N-acetyl-deoxyMonosaccharide ( hexose) 
Method of Glycan IndentificationLC-MS, and MS-MS (CID and HCD)
Acceptor Subtrate Information
Acceptor Substrate name PilA
ProGPdb ID ProGP419
Litrature
Year Of Validation2015 
Reference Harding, C.M., Nasr, M.A., Kinsella, R.L., Scott, N.E., Foster, L.J., Weber, B.S., Fiester, S.E., Actis, L.A., Tracy, E.N., Munson Jr, R.S. & Feldman, M. F. (2015). A cinetobacter strains carry two functional oligosaccharyltransferases, one devoted exclusively to type IV pilin, and the other one dedicated to O?glycosylation of multiple proteins. Molecular microbiology, 96(5), 1023-1041.

Authors Harding, C.M., Nasr, M.A., Kinsella, R.L., Scott, N.E., Foster, L.J., Weber, B.S., Fiester, S.E., Actis, L.A., Tracy, E.N., Munson Jr, R.S. & Feldman, M. F.
Research groups1 Center for Microbial Pathogenesis, The Research Institute at Nationwide Childrens Hospital, Columbus, OH, USA. 2 Department of Pediatrics, College of Medicine, The Ohio State University, Columbus, OH, USA. 3 Biomedical Sciences Graduate Program, College of Medicine, The Ohio State University, Columbus, OH, USA. 4 Department of Biological Sciences, University of Alberta, Edmonton, AB, T6G 2G2, Canada. 5 Centre for High-Throughput Biology, University of British Columbia, Vancouver, BC, Canada. 6 Department of Microbiology, Miami University, Oxford, OH, USA
Corresponding Author Feldman, M. F.
ContactsDepartment of Biological Sciences, University of Alberta, Edmonton, AB, T6G 2G2, Canada.