Latest update: September 24, 2018


ProGT92 (PglB)

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ProGT ID ProGT92 (PglB)
Organism Information
Organism NameCampylobacter fetus venerealis
Clinical ImplicationPathogenic
DomainBacteria
PhylumProteobacteria
ClassificationFamily: Campylobacteraceae
Order: Campylobacterales
Class: Epsilonproteobacteria
Division or phylum: "Proteobacteria"
Taxonomic ID (NCBI)1240980
Genome Information
Gene BankCP008808
EMBLCP008808
Gene Information
Gene NamepglB
NCBI Reference SequenceAIR81123.1
Protein information
Protein NamePglB 
UniProtKB/ SwissProt IDA0A0E1GJK1
NCBI Ref SeqWP_002850224.1
UniProtKB Sequence>tr|A0A0E1GJK1|A0A0E1GJK1_CAMFE Undecaprenyl-diphosphooligosaccharide--protein glycotransferase OS=Campylobacter fetus subsp. fetus 04/554 GN=pglB PE=4 SV=1 MKNLGNFFMIFRSNTLFLIILAYIFSVICRFEWIYWASGYAEFFWNNQLMISTNDGYAYA EGARDMIAGFHQPNDLSFYGTPLSSITYLLVEITPFSLETIMVYLSVFLGGLVVIPIILM AREIGEIKAGFIAALLASIANSYYNRTMAGYYDTDMLIISLPIFTFWGIVRLYTKRDLKS FLIISFSMLIYSWWYPSSFSLNVAITLFFGLYTIIFDRKNILNYQAVLFMILAISGAEYF IRAILILALFVFIKFKPNLINTKVMISLYAAVLLFFIYFGGLNPIWFQLKFYIFRSVSDS DGVMFKYFNVNQTIMESGMIDFRFFAQRISGDIFVFLIALIGYVLLCFKYRIFLVTLPMI GLGFLSLKGGLRFTIYAVPMMAFGFGYALNWISKFIKIDKKHRKISLYAVTFFSFLPVIL LVLLQIYEGIYLDEFKFLFPIFVVAYILFYFKFRREIATMVLFPLSLFALTPSLIHIYEY KPLTVFMNSEVKVLDKLKTIADPEDYVVAWWDYGYAIRYYSDTKTLIDGGKHLGRENFAV SWALSRPFISSANMARLEVEYTERGFEQKFNSNLNEMLKDYNASDVNGFLASLNDQNFTL PEKTRDIYYYLPDRMMGIFPVVTKFSRLDLVTAKEFRDAFFIVSTSFAQGGDGVVLSEGI TIANDGTKISFSGKTFRVNSYIETKYDKNMNLEKQIHHIDDNGGFYVVYMVDYGRFLIMD KEMFNSTYIQLFVLENYEGSDYEPIILDPSAKVYKLKR
EMBL CDSAIR79271.1
Sequence length758 AA
Subcellular LocationMembrane (Integral component of membrane)
Glycosyltransferase Information
Glycosylation TypeN- (Asn) linked 
CAZY FamilyGT66
EC Number (BRENDA)2.4.99.18
Mechanism of Glycan TransferEn bloc
Acceptor specificity Sequon_1Asp/Glu- Asn-Xaa-Ser/Thr
Donor TypeLipid linked sugars
Donor SpecificityUndPP-Heptasaccharide
Glycan Information
Glycan transferredHeptasaccharide 
Method of Glycan IndentificationLC-MS
Experimental_strategiesIn vivo and In vitro 
Acceptor Subtrate Information
Acceptor Substrate name scFv13-R4 DQNAT
Acceptor Substrate name TAMRA-GDQNATAF
Litrature
Year Of Validation2015 
Reference Ollis, A.A., Chai, Y., Natarajan, A., Perregaux, E., Jaroentomeechai, T., Guarino, C., Smith, J., Zhang, S. & DeLisa, M. P. (2015). Substitute sweeteners: diverse bacterial oligosaccharyltransferases with unique N-glycosylation site preferences. Scientific reports, 5, 15237.

Authors Ollis, A.A., Chai, Y., Natarajan, A., Perregaux, E., Jaroentomeechai, T., Guarino, C., Smith, J., Zhang, S. & DeLisa, M. P.
Research groups1 School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853 USA. 2 Department of Microbiology, Cornell University, Ithaca, NY 14853 USA. 3 Comparative Biomedical Sciences, Cornell University, Ithaca, NY 14853 USA. 4 Proteomics and Mass Spectrometry Core Facility, Cornell University, Ithaca, New York.
Corresponding Author DeLisa, M. P.
ContactsProteomics and Mass Spectrometry Core Facility, Cornell University, Ithaca, New York 14853.