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ProGlyProt ID
BU112
Organism Information
Organism Name
Bacillus circulans K-1
Domain
Bacteria
Classification
Family: Bacillaceae
Order: Bacillales
Class: Bacilli (or Firmibacteria)
Division or phylum: "Firmicutes"
Taxonomic ID (NCBI)
 
 
Genome Sequence(s)
EMBL
 
 
Gene Information
Gene Name
G-gene
 
 
Protein Information
Protein Name
β-1,4-mannanase (G-enzyme)
UniProtKB/SwissProt ID
EMBL-CDS
UniProtKB Sequence
Sequence length
516 AA
Subcellular Location
Intracellular
Function
Degrades guar gum which consists of a galactomannosaccharide from the endosperm of guar seeds.
 
 
Glycosylation Status
Glycosylation Type
Information currently not available with us.
Technique(s) used for Glycosylation Detection
Mass shift detected on SDS-polyacrylamide gel, periodic acid-Schiff (PAS) staining
 
 
Glycan Information
Glycan Annotation
Recombinant G-enzyme (expressed in E. coli) shows GalNAc, Xyl, GlcNAc, Man, Glc, and Gal to account for 1.7%, 14.4%, 6.1%, 3.2%, 54.2%, and 10.4%, respectively, of total monosaccharides. G-enzyme from strain K-1 contains GalNAc, Xyl, GlcNAc, Man, Fuc, and Gal that account for 1.8%, 0.9%, 11.0%, 6.1%, 1.1%, and 79.1%, respectively, of total monosaccharides.
 
 
Literature
Reference(s)
1) Yoshida, S., Sako, Y. and Uchida, A. (1998) Cloning, sequence analysis, and expression in Escherichia coli of a gene coding for an enzyme from Bacillus circulans K-1 that degrades guar gum. Biosci Biotechnol Biochem, 62, 514-520. [PubMed: 9571781]
Year of Identification
1998
 
 
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