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ProGlyProt ID
BU151
Organism Information
Organism Name
Corynebacterium glutamicum (Brevibacterium flavum) ATCC 13032
Domain
Bacteria
Classification
Family: Corynebacteriaceae
Suborder: Corynebacterineae
Order: Actinomycetales
Subclass: Actinobacteridae
Class: Actinobacteria
Division or phylum: "Actinobacteria"
Taxonomic ID (NCBI)
 
 
Genome Sequence(s)
GenBank
EMBL
 
 
Gene Information
Gene Name
rpf2 (cg1037)
NCBI Gene ID
GenBank Gene Sequence
 
 
Protein Information
Protein Name
Resuscitation promoting factor 2 (Rpf2)
UniProtKB/SwissProt ID
NCBI RefSeq
EMBL-CDS
UniProtKB Sequence
Sequence length
374 AA
Subcellular Location
Cell surface (secreted)
Function
Rpf2 plays a role in intercellular communication and growth stimulation of C. glutamicum.
 
 
Glycosylation Status
Glycosylation Type
Information currently not available with us.
Technique(s) used for Glycosylation Detection
DIG (digoxigenin) glycan detection using anti-DIG antibody
 
 
Glycan Information
Glycan Annotation
Galactose and mannose are the constituents of the oligosaccharide that were identified by GCMS.
 
 
Literature
Reference(s)
1) Mahne, M., Tauch, A., Puhler, A. and Kalinowski, J. (2006) The Corynebacterium glutamicum gene pmt encoding a glycosyltransferase related to eukaryotic protein-O-mannosyltransferases is essential for glycosylation of the resuscitation promoting factor (Rpf2) and other secreted proteins. FEMS Microbiol Lett, 259, 226-233. [PubMed: 16734784]
2) Hartmann, M., Barsch, A., Niehaus, K., Puhler, A., Tauch, A. and Kalinowski, J. (2004) The glycosylated cell surface protein Rpf2, containing a resuscitation-promoting factor motif, is involved in intercellular communication of Corynebacterium glutamicum. Arch Microbiol, 182, 299-312. [PubMed: 15480574]
Additional Comments
Rpf2 is the first example of a glycoprotein in C. glutamicum.
Protein mannosyltransferase, pmt gene (cg1014) has been shown to glycosylate secreted proteins including Rpf2 by gene deletion studies. This gene has also been found to possess significant similarity to eukaryotic PMTs and an orthologue of M. tuberculosis, Rv1002c.
Year of Identification
2004
 
 
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