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ProGlyProt ID
BU272
Organism Information
Organism Name
Streptomyces coelicolor J1929
Domain
Bacteria
Classification
Family: Streptomycetaceae
Suborder: Streptomycineae
Order: Actinomycetales
Subclass: Actinobacteridae
Class: Actinobacteria
Division or phylum: "Actinobacteria"
Taxonomic ID (NCBI)
 
 
Genome Sequence(s)
GenBank
EMBL
 
 
Gene Information
Gene Name
pstS (SCO4142)
NCBI Gene ID
 
 
Protein Information
Protein Name
PstS (40 kDa)
UniProtKB/SwissProt ID
NCBI RefSeq
EMBL-CDS
UniProtKB Sequence
Sequence length
370 AA
Subcellular Location
Secreted
Function
Phosphate binding protein involved in high affinity phosphate uptake.
 
 
Glycosylation Status
Glycosylation Type
Information currently not available with us.
Technique(s) used for Glycosylation Detection
Pro-Q Emerald Glycoprotein staining (that uses periodate oxidation) and ConA–sepharose affinity chromatography.
 
 
Glycan Information
Glycan Annotation
Trihexose (mannose).
 
 
Literature
Reference(s)
1) Wehmeier, S., Varghese, A.S., Gurcha, S.S., Tissot, B., Panico, M., Hitchen, P., Morris, H.R., Besra, G.S., Dell, A. and Smith, M.C. (2009) Glycosylation of the phosphate binding protein, PstS, in Streptomyces coelicolor by a pathway that resembles protein O-mannosylation in eukaryotes. Mol Microbiol, 71, 421-433. [PubMed: 19017269]
Additional Comments
Both Pmt (putative protein O-mannosyltransferase) and Ppm1 (polyprenol phosphate mannose synthase) are required for glycosylation. Ppm1 transfers mannose to endogenous polyprenol phosphate in membrane preparations. Ppm1 catalyzes mannose transfer.
Year of Identification
2009
 
 
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