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ProGlyProt ID
Organism Information
Organism Name
Thermoanaerobacterium (formerly Clostridium) thermosaccharolyticum E207-71
Family: Thermoanaerobacteraceae
Order: Thermoanaerobacterales
Class: Clostridia
Division or phylum: "Firmicutes"
Protein Information
Protein Name
S-layer glycoprotein
Subcellular Location
Structural component of the surface layer.
Glycosylation Status
Glycosylation Type
O(Tyr)- linked
Technique(s) used for Glycosylation Detection
Chemical deglycosylation with TFMS
Glycan Information
Glycan Annotation
Linkages: β-Glc-O-Tyr, β-Gal-O-Tyr. Glucosyl tyrosine is found in short-chain glycans while galactosyl tyrosine in long-chain glycans. It is the first report where the unusual monosaccharide 3-acetamido-3,6-dideoxy-D-glucose (quinovosamine) has been found to be present in S-layer glycoproteins. Repeating hexasaccharide [→4)[β-D-Quip3NAc-(1→6)-β-D-Galf-(1→4)-α-L-Rhap-(1→3)]-α-D-Manp-(1→4)-β-D-Galp-(→4)-β-D-Glcp-(1→]n=17 is present. Microheterogeneity is also observed.
1) Messner, P., Steiner, K., Zarschler, K. and Schaffer, C. (2008) S-layer nanoglycobiology of bacteria. Carbohydr Res, 343, 1934-1951. [PubMed: 18336801]
2) Altman, E., Schaffer, C., Brisson, J.R. and Messner, P. (1996) Isolation and characterization of an amino sugar-rich glycopeptide from the surface layer glycoprotein of Thermoanaerobacterium thermosaccharolyticum E207-71. Carbohydr Res, 295, 245-253. [PubMed: 9002194]
3) Altman, E., Schaffer, C., Brisson, J.R. and Messner, P. (1995) Characterization of the glycan structure of a major glycopeptide from the surface layer glycoprotein of Clostridium thermosaccharolyticum E207-71. Eur J Biochem, 229, 308-315. [PubMed: 7744045]
Additional Comments
100-210 kDa ladder-like bands represented glycosylated S-layer subunits.
Year of Identification
Copyright @ 2011 IMTECH
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