| Technique(s) used for Glycosylation Detection | Carbohydrate analysis using trifluoroacetic acid and GC/MS (gas chromatography/mass spectrometry). |
| Technique(s) used for Glycosylated Residue(s) Detection | Edman degradation |
| Glycan Information |
| Glycan Annotation | Carbohydrates present: glucose, mannose, and glucosamine. Both N and O linked glycans present.
β-elimination revealed that at least 15 carbohydrate chains are bound to Ser or Thr. Two molecules of 150 KDa proteases are associated with glycosylated tetrabrachion polypeptides (85 KDa & 92KDa) and for this tetrabrachion-protease complex overall carbohydrate content is 38%. |
| Literature |
| Year of Identification | 1995 |
| Year of Identification Month Wise | 1995.01.01 |
| Year of Validation | 1996 |
| Reference | Özbek, S., Müller, J.F., Figgemeier, E. and Stetefeld, J., 2005. Favourable mediation of crystal contacts by cocoamidopropylbetaine (CAPB). Acta Crystallographica Section D: Biological Crystallography, 61(4), pp.477-480. |
| Corresponding Author | Jörg Stetefeld |
| Contact | Department of Biophysical Chemistry, Biozentrum, University of Basel, Klingelbergstrase 70, CH-4056 Basel, Switzerland. |
| Reference | Stetefeld, J., Jenny, M., Schulthess, T., Landwehr, R., Engel, J. and Kammerer, R.A., 2000. Crystal structure of a naturally occurring parallel right-handed coiled coil tetramer. Nature structural biology, 7(9), pp.772-776. |
| Corresponding Author | Jörg Stetefeld |
| Contact | Department of Biophysical Chemistry, Biozentrum, University of Basel, Klingelbergstrase 70, CH-4056 Basel, Switzerland. |
| Reference | Peters, J., Baumeister, W. and Lupas, A., 1996. Hyperthermostable Surface Layer Protein Tetrabrachion from the ArchaebacteriumStaphylothermus marinus: Evidence for the Presence of a Right-handed Coiled Coil Derived from the Primary Structure. Journal of molecular biology, 257(5), pp.1031-1041. |
| Corresponding Author | Jurgen Peters |
| Contact | Max-Planck-Institut für Biochemie, Martinsried, Germany. |
| Reference | Peters, J., Nitsch, M., Kühlmorgen, B., Golbik, R., Lupas, A., Kellermann, J., Engelhardt, H., Pfander, J.P., Müller, S., Goldie, K. and Engel, A., 1995. Tetrabrachion: a filamentous archaebacterial surface protein assembly of unusual structure and extreme stability. Journal of molecular biology, 245(4), pp.385-401. |
| Corresponding Author | Jurgen Peters |
| Contact | Max-Planck-Institut für Biochemie, Martinsried, Germany. |
| Reference | Ozbek, S., Muller, J.F., Figgemeier, E. and Stetefeld, J. (2005) Favourable mediation of crystal contacts by cocoamidopropylbetaine (CAPB). Acta Crystallogr D Biol Crystallogr, 61, 477-480. [PubMed: 15805603] |
| Author | Ozbek, S., Muller, J.F., Figgemeier, E. and Stetefeld, J. |
| Research Group | Department of Biophysical Chemistry, Biozentrum, University of Basel, Klingelbergstrase 70, CH-4056 Basel, Switzerland. |
| Corresponding Author | Stetefeld, J. |
| Contact | Department of Biophysical Chemistry, Biozentrum, University of Basel, Klingelbergstrase 70, CH-4056 Basel, Switzerland. |
| Reference | Stetefeld, J., Jenny, M., Schulthess, T., Landwehr, R., Engel, J. and Kammerer, R.A. (2000) Crystal structure of a naturally occurring parallel right-handed coiled coil tetramer. Nat Struct Biol, 7, 772-776. [PubMed: 10966648] |
| Author | Stetefeld, J., Jenny, M., Schulthess, T., Landwehr, R., Engel, J. and Kammerer, R.A. |
| Research Group | Department of Biophysical Chemistry, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland. |
| Corresponding Author | Kammerer, R.A. |
| Contact | Department of Biophysical Chemistry, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland. joerg.stetefeld@unibas.ch |
| Reference | Stetefeld, J., Jenny, M., Schulthess, T., Landwehr, R., Engel, J. and Kammerer, R.A. (2000) Crystal structure of a naturally occurring parallel right-handed coiled coil tetramer. Nat Struct Biol, 7, 772-776. [PubMed: 10966648] |
| Author | Stetefeld, J., Jenny, M., Schulthess, T., L wehr, R., Engel, J. |
| Research Group | Department of Biophysical Chemistry, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland. |
| Corresponding Author | wehr, R., Engel, J. |
| Contact | Department of Biophysical Chemistry, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland. |
| Reference | Peters, J., Baumeister, W. and Lupas, A. (1996) Hyperthermostable surface layer protein tetrabrachion from the archaebacterium Staphylothermus marinus: evidence for the presence of a right-handed coiled coil derived from the primary structure. J Mol Biol, 257, 1031-1041. [PubMed: 8632466] |
| Author | Peters, J., Baumeister, W. Lupas, A. |
| Research Group | Max Planck Institute of Biochemistry, Martinsried Germany. |
| Corresponding Author | Lupas, A. |
| Contact | Max Planck Institute of Biochemistry, Martinsried Germany. |
| Reference | Peters, J., Nitsch, M., Kuhlmorgen, B., Golbik, R., Lupas, A., Kellermann, J., Engelhardt, H., Pfander, J.P., Muller, S., Goldie, K. et al. (1995) Tetrabrachion: a filamentous archaebacterial surface protein assembly of unusual structure and extreme stability. J Mol Biol, 245, 385-401. [PubMed: 7837271] |
| Author | Peters, J., Nitsch, M., Kuhlmorgen, B., Golbik, R., Lupas, A., Kellermann, J., Engelhardt, H., Pfander, J.P., Muller, S., Goldie, K. et al. |
| Research Group | Structural Biochemistry Group, Michael Swann |
| Corresponding Author | Ju¨rgen Peters |
| Contact | Structural Biochemistry Group, Michael Swann |
