ProGP1163 (Mpsy_1486)
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| ProGP ID | ProGP1163 (Mpsy_1486) |
| Validation Status | Characterized |
| Organism Information | |
| Organism Name | Methanolobus psychrophilus |
| Domain | Archaea |
| Classification | Phylum : Euryarchaeota Class : Methanomicrobia Orders : Methanosarcinales Family : Methanosarcinaceae Genus : Methanolobus Species : psychrophilus |
| Taxonomic ID (NCBI) | 1094980 |
| Genome Information | |
| GenBank | CP003083.1 |
| Protein Information | |
| Protein Name | Mpsy_1486 |
| NCBI RefSeq | AFV23693 |
| UniProtKB Sequence | >AFV23693.1 hypothetical protein Mpsy_1486 [Methanolobus psychrophilus R15] MTKLTTTVVVALMIMALTIMSASAATSVEVRSSVLTGPDTLDATNFAGFYYDLDDNISTETMEITAISTRTIAAGALKYNTTIAAVDYASSNLNITGSPKYNVMGLFAEKYIPLDSTTPDKLSKLLLDTSDKYTLRTGTALQLPEGYALTAKQIDVEGNKVWMEFTKDGEFVEDEILDVSGGKAVWTYEADDIAGEDDVIVMKVAVTNVFQGQVDSLAVIEGVWLMDYENVMEIETDDTFDKLEVVTVGPGLSMENSNAITLTRDKTISIAQGMSFKVADDSAVRFYVMKVFTEPGTYDIRGQVASGTGSQTWTAVNFAGFYYDLDDDISTESMEITGISIRTIAAGALKYNTTIAAVDYASSNLNTTSNPKYNVMGLFAEKYIPLDSTTPDKLSKLLLDSSDKYTLRTGTALQLPEGYALTAKQIDVEGNKVWMEFTKDGEFVEDEILDVSNGTVVWTYEADDIAGEDDVIVMKVAVTNVFQGQVDSLAVIEGVWLMDYENVMEIETDDTFDKLEVVTVGPGLSMENSNAITLTRDKTISIAQGMSFKVADDSAVRFYVMKVFTEPGTYDIRGQVASGTGSQTWTAVNFAGFYYDLDDDISTESMEITGISIRTIAAGALKYNTTIAAVDYASSNLNTTSNPKYNVMGLFAEKYIPLDSTTPDKLSKLLLDSSDKYTLRTGTALQLPEGYALTAKQIDVEGNKVWMEFTKDGEFVEDEILDVSNGTVVWTYEADDIAGEDDVIVMKVAVTNVFQGQVDSLAVVEGVWLMDYENVMEIETDDTFDKLEVVTVGPGLSMENSNAITLTRDKVISIAQGMNFKVADDSNLRYYPFATLTIEGDGVTQPETPVDETPVDNGNETPVDETPVDETPVDETPGNETPVETPEEESPGFEAIFAIAGLLAVAYLVRRN |
| Subcellular Location | S-layer of cell wall |
| Function | Maintaining stability and integrity of cell wall, adapting pychrophilic environment |
| Glycosylation Status | |
| Glycosylation Type | N- (Asn) linked |
| Experimentally Predicted Glycosites | N-94, N-453, N-725, N-879 |
| Experimentally Validated Glycosite(s) in Full Length Protein | N-94, N-453, N-725, N-879 |
| Experimentally Validated Glycosite(s ) in Mature Protein | >AFV23693.1 hypothetical protein Mpsy_1486 [Methanolobus psychrophilus R15] MTKLTTTVVVALMIMALTIMSASAATSVEVRSSVLTGPDTLDATNFAGFYYDLDDNISTETMEITAISTRTIAAGALKYNTTIAAVDYASSNLN*(94)ITGSPKYNVMGLFAEKYIPLDSTTPDKLSKLLLDTSDKYTLRTGTALQLPEGYALTAKQIDVEGNKVWMEFTKDGEFVEDEILDVSGGKAVWTYEADDIAGEDDVIVMKVAVTNVFQGQVDSLAVIEGVWLMDYENVMEIETDDTFDKLEVVTVGPGLSMENSNAITLTRDKTISIAQGMSFKVADDSAVRFYVMKVFTEPGTYDIRGQVASGTGSQTWTAVNFAGFYYDLDDDISTESMEITGISIRTIAAGALKYNTTIAAVDYASSNLNTTSNPKYNVMGLFAEKYIPLDSTTPDKLSKLLLDSSDKYTLRTGTALQLPEGYALTAKQIDVEGNKVWMEFTKDGEFVEDEILDVSN*(453)GTVVWTYEADDIAGEDDVIVMKVAVTNVFQGQVDSLAVIEGVWLMDYENVMEIETDDTFDKLEVVTVGPGLSMENSNAITLTRDKTISIAQGMSFKVADDSAVRFYVMKVFTEPGTYDIRGQVASGTGSQTWTAVNFAGFYYDLDDDISTESMEITGISIRTIAAGALKYNTTIAAVDYASSNLNTTSNPKYNVMGLFAEKYIPLDSTTPDKLSKLLLDSSDKYTLRTGTALQLPEGYALTAKQIDVEGNKVWMEFTKDGEFVEDEILDVSN*(725)GTVVWTYEADDIAGEDDVIVMKVAVTNVFQGQVDSLAVVEGVWLMDYENVMEIETDDTFDKLEVVTVGPGLSMENSNAITLTRDKVISIAQGMNFKVADDSNLRYYPFATLTIEGDGVTQPETPVDETPVDNGNETPVDETPVDETPVDETPGN*(879)ETPVETPEEESPGFEAIFAIAGLLAVAYLVRRN |
| Glycosite(s) Annotated Protein Sequence | AAVDYASSNLNITGSPKYNVM FVEDEILDVSNGTVVWTYEAD FVEDEILDVSNGTVVWTYEAD PVDETPVDNGNETPVDETPVD |
| Technique(s) used for Glycosylation Detection | Western blotting |
| Protein Glycosylation- Implication | Four N-glycosylation sites were identified on Mpsy_1486, which exhibited different glycosylation profiles, with N94 only found in cells cultured at 30 °C. An N-linked glycosylation inhibitor, tunicamycin, reduced glycosylation levels of Mpsy_1486 and growth at 30 °C, thus establishing a link between S-layer protein glycosylation and higher temperature adaptation of the psychrophilic archaeon M. psychrophilus. |
| Glycan Information | |
| Glycan Annotation | (GlcNAc-135)2-Hex-HexA |
| Technique(s) used for Glycan Identification | Western blotting, Nano-LC-ESIMS/MS analysis |
| Literature | |
| Year of Identification | 2019 |
| Reference | Li, L., Ren, M., Xu, Y., Jin, C., Zhang, W. and Dong, X., 2020. Enhanced glycosylation of an S‐layer protein enables a psychrophilic methanogenic archaeon to adapt to elevated temperatures in abundant substrates. FEBS letters, 594(4), pp.665-677. |
| Corresponding Author | Xiuzhu Dong |
| Contact | 1.State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China 2.University of Chinese Academy of Sciences, Beijing, China |