ProGP121 (Surface layer glycoprotein Sat B)
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ProGP ID | ProGP121 (Surface layer glycoprotein Sat B) |
Validation Status | Characterized |
Organism Information | |
Organism Name | Aneurinibacillus thermoaerophilus DSM 10155 |
Domain | Bacteria |
Classification | Family: Paenibacillaceae Order: Bacillales Class: Bacilli (or Firmibacteria) Division or phylum: "Firmicutes" |
Taxonomic ID (NCBI) | 143495 |
Genome Information | |
GenBank | AY395579 |
EMBL | AY395579 |
Gene Information | |
Gene Name | sat B |
Protein Information | |
Protein Name | Surface-layer glycoprotein Sat B |
UniProtKB/SwissProt ID | Q6TL21 |
EMBL-CDS | AAS44592.1 |
UniProtKB Sequence | >tr|Q6TL21|Q6TL21_ANETH Surface-layer glycoprotein SatB OS=Aneurinibacillus thermoaerophilus GN=satB PE=3 SV=1 MKTNKALKVLTTSALLASVAAPFGAGVVSANITNLVNKVLNVKSNYDGEQSASHTLTVKE DDIAFDQTSGETFRVVLPSGVKWKTAPANTPTAKFDLRSDQVLEVTVKGTNTIADDEQVS IPLNFKVDGATGELKLKVESMDSTITSGEYVFAIVGSGSTTAAVDNVKNIGKSGKAGTIR VEETAIGAVADGRNHKVKLKLPSNFRWVTTGTDKTEISTSGGFTSASIVTGESNDNVLVL DVATATGQGRGVLYVTPYIKADKDASYGDVSVNIRGPDFSDSDVVVAKYGDWGATIKVEK VEEVLAGKYDENTRTAKITIEESVPGSFVKGRDIDVELPSWVKVTDIKEFTTSGFTTGAP TSGSVDIDGTDNKISIPLGETGGSSTAKIEFKLELSVQADKTGDIEAVISGGGVAEQKAV IAKAVAPVSVALDGEASKVKIGAQAQDAPNIIITELKKGAIEKTTENGSTTNGTIEVKLP DGVKFASVPKVEVVEGDGTIDVDNVRRINNDNVLTIPVKGESVSKPMKIKLSNVKLTVDR TVPEGDIELKVGGNAIVENYKSGKGWLKGNKQNGSSDALDAGEFNTEHAAKVVVAKTVTP APTDTTATNIVFKLNSKTYTVDGKEFEMDAAPLVGWDRAYLPVRFAANALNVSDDQIVWD DKTSTFTIFKGDRVISGKVGDKFLTVNGAKVPMDVPVWRNKEKTNNRVMVPIRYLANALN AEIEWNKETSEITIQAKK |
Sequence length | 738 AA |
Subcellular Location | Surface |
Glycosylation Status | |
Glycosylation Type | O- (Ser/Thr) linked |
Experimentally Validated Glycosite(s) in Full Length Protein | T471 and/or S69 |
Experimentally Validated Glycosite(s ) in Mature Protein | T471 and /or S69 |
Glycosite(s) Annotated Protein Sequence | >tr|Q6TL21|Q6TL21_ANETH Surface-layer glycoprotein SatB OS=Aneurinibacillus thermoaerophilus GN=satB PE=3 SV=1 MKTNKALKVLTTSALLASVAAPFGAGVVSANITNLVNKVLNVKSNYDGEQSASHTLTVKE DDIAFDQTS*(69)GETFRVVLPSGVKWKTAPANTPTAKFDLRSDQVLEVTVKGTNTIADDEQVS IPLNFKVDGATGELKLKVESMDSTITSGEYVFAIVGSGSTTAAVDNVKNIGKSGKAGTIR VEETAIGAVADGRNHKVKLKLPSNFRWVTTGTDKTEISTSGGFTSASIVTGESNDNVLVL DVATATGQGRGVLYVTPYIKADKDASYGDVSVNIRGPDFSDSDVVVAKYGDWGATIKVEK VEEVLAGKYDENTRTAKITIEESVPGSFVKGRDIDVELPSWVKVTDIKEFTTSGFTTGAP TSGSVDIDGTDNKISIPLGETGGSSTAKIEFKLELSVQADKTGDIEAVISGGGVAEQKAV IAKAVAPVSVALDGEASKVKIGAQAQDAPNIIITELKKGAIEKTTENGSTT*(471)NGTIEVKLP DGVKFASVPKVEVVEGDGTIDVDNVRRINNDNVLTIPVKGESVSKPMKIKLSNVKLTVDR TVPEGDIELKVGGNAIVENYKSGKGWLKGNKQNGSSDALDAGEFNTEHAAKVVVAKTVTP APTDTTATNIVFKLNSKTYTVDGKEFEMDAAPLVGWDRAYLPVRFAANALNVSDDQIVWD DKTSTFTIFKGDRVISGKVGDKFLTVNGAKVPMDVPVWRNKEKTNNRVMVPIRYLANALN AEIEWNKETSEITIQAKK |
Sequence Around Glycosites (21 AA) | IEKTTENGSTTNGTIEVKLPD
KEDDIAFDQTSGETFRVVLPS |
Technique(s) used for Glycosylation Detection | Periodic acid-Schiff (PAS) staining |
Technique(s) used for Glycosylated Residue(s) Detection | Modified Edman degradation |
Glycan Information | |
Glycan Annotation | Linkage: ?-GalNAc-Ser/Thr. Total carbohydrate content of 15%. Disaccharide repeating unit composed of L-rhamnose and D-glycero-D-manno-heptose residues: ?4)-?-L-Rhap-(1?3)-?-D-glycero-D-manno-Hepp-(1? and ?-D-GalpNAc-(1?O)-Ser/Thr linkage. Equal amounts of the complete core structure ?-L-Rhap-(1?3)-?-L-Rhap-(1?3)-?-D-GalpNAc-(1?O)-Ser/Thr and the truncated forms ?-L-Rhap-(1?3)-?-D-GalpNAc-(1?O)-Ser/Thr and ?-D-GalpNAc-(1?O)-Ser/Thr are present. The different cores were substituted with varying numbers of disaccharide repeating units. |
BCSDB ID | 23143 943 |
GlyTouCan | G56797IU G29447EH |
Literature | |
Year of Identification | 1995 |
Year of Identification Month Wise | 1995.12.25 |
Year of Validation | 1999 |
Reference | Messner, P., Steiner, K., Zarschler, K. and Schaffer, C. (2008) S-layer nanoglycobiology of bacteria. Carbohydr Res, 343, 1934-1951. [PubMed: 18336801] |
Author | Messner, P., Steiner, K., Zarschler, K. and Schaffer, C |
Research Group | University of Natural Resources and Applied Life Sciences, Vienna, Center for NanoBiotechnology A-1180 Vienna, Gregor-Mendel-Strasse 33, Austria. |
Corresponding Author | Schaffer, C. |
Contact | University of Natural Resources and Applied Life Sciences, Vienna, Center for NanoBiotechnology A-1180 Vienna, Gregor-Mendel-Strasse 33, Austria. |
Reference | Kneidinger, B., Graninger, M., Puchberger, M., Kosma, P. and Messner, P. (2001) Biosynthesis of nucleotide-activated D-glycero-D-manno-heptose. J Biol Chem, 276, 20935-20944. [PubMed: 11279237] |
Author | Kneidinger, B., Graninger, M., Puchberger, M., Kosma, P. and Messner, P. |
Research Group | Center for Ultrastructure Research and Ludwig Boltzmann Institute for Molecular Nanotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Gregor-Mendel-Strasse 33, A-1180 Vienna, Austria. |
Corresponding Author | Messner, P. |
Contact | Center for Ultrastructure Research and Ludwig Boltzmann Institute for Molecular Nanotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Gregor-Mendel-Strasse 33, A-1180 Vienna, Austria. |
Reference | Wugeditsch, T., Zachara, N.E., Puchberger, M., Kosma, P., Gooley, A.A. and Messner, P. (1999) Structural heterogeneity in the core oligosaccharide of the S-layer glycoprotein from Aneurinibacillus thermoaerophilus DSM 10155. Glycobiology, 9, 787-795.[PubMed: 10406844] |
Author | Wugeditsch, T., Zachara, N.E., Puchberger, M., Kosma, P., Gooley, A.A. Messner, P. |
Research Group | Center for Ultrastructure Research and Ludwig Boltzmann Institute for Molecular Nanotechnology, University of Soil Culture, A-1180 Vienna, Austria. |
Corresponding Author | Messner, P. |
Contact | Center for Ultrastructure Research and Ludwig Boltzmann Institute for Molecular Nanotechnology, University of Soil Culture, A-1180 Vienna, Austria. |
Reference | Kosma, P., Wugeditsch, T., Christian, R., Zayni, S. and Messner, P. (1995) Glycan structure of a heptose-containing S-layer glycoprotein of Bacillus thermoaerophilus. Glycobiology, 5, 791-796. [PubMed: 8720077] |
Author | Kosma, P., Wugeditsch, T., Christian, R., Zayni, S. Messner, P. |
Research Group | Institute of Chemistry, University of Natural Resources and Applied Life Sciences, Vienna, Austria |
Corresponding Author | Messner, P. |
Contact | Institute of Chemistry, University of Natural Resources and Applied Life Sciences, Vienna, Austria |