ProGP1231 (CbFla)
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| ProGP ID | ProGP1231 (CbFla) |
| Validation Status | Characterized |
| Organism Information | |
| Organism Name | Clostridium botulinum |
| Domain | Bacteria |
| Classification | Phylum : Firmicutes Class : Clostridia Orders : Eubacteriales Family : Clostridiaceae Genus : Clostridium Species : botulinum |
| Taxonomic ID (NCBI) | 441772 |
| Gene Information | |
| Gene Name | flaA2 |
| Protein Information | |
| Protein Name | CbFlaA |
| NCBI RefSeq | WP_003359201.1 |
| UniProtKB Sequence | >WP_003359201.1 flagellin [Clostridium botulinum] MIINHNLNAMNAHRQMAINTGSNGKAIEKLSSGLRINRAGDDAAGLAISEKMRGQIRGLNQASRNSQDSI SLIQTAEGALNETHSILQRMRELAVQSANDTNVKVDRDNLQKEVAELQNEINRIASQTQFNTKNLLNGSA KSMVFQIGANKDQVMELTIAGMGTSALKVGSINIGSGKGAKSISGMIETIDNAINTVSGERAKLGANQNR LEHTIANLDNSAENLQAAESRIRDVDMAKEMMNFTKTNILTQAAQAMLAQANQAPQGVLQLLR |
| Function | Required for motility and virulence |
| Glycosylation Status | |
| Glycosylation Type | O- (Ser/Thr) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | S142 |
| Experimentally Validated Glycosite(s ) in Mature Protein | >WP_003359201.1 flagellin [Clostridium botulinum] MIINHNLNAMNAHRQMAINTGSNGKAIEKLSSGLRINRAGDDAAGLAISEKMRGQIRGLNQASRNSQDSI SLIQTAEGALNETHSILQRMRELAVQSANDTNVKVDRDNLQKEVAELQNEINRIASQTQFNTKNLLNGSA KS*(142)MVFQIGANKDQVMELTIAGMGTSALKVGSINIGSGKGAKSISGMIETIDNAINTVSGERAKLGANQNR LEHTIANLDNSAENLQAAESRIRDVDMAKEMMNFTKTNILTQAAQAMLAQANQAPQGVLQLLR |
| Glycosite(s) Annotated Protein Sequence | TKNLLNGSAKSMVFQIGANKD |
| Technique(s) used for Glycosylation Detection | LC–MS/MS and Western blotting |
| Glycan Information | |
| Glycan Annotation | 3-deoxy-D-manno-octulosonic acid or keto-deoxyoctulosonate (KDO) |
| Technique(s) used for Glycan Identification | LC–MS/MS and Western blooting |
| Protein Glycosylation linked (PGL) gene(s) | |
| OST ProGT ID | ProGT136 (Maf) |
| Literature | |
| Year of Identification | 2021 |
| Reference | Khairnar, A., Sunsunwal, S., Babu, P. and Ramya, T.N.C., 2021. Novel serine/threonine-O-glycosylation with N-acetylneuraminic acid and 3-deoxy-D-manno-octulosonic acid by bacterial flagellin glycosyltransferases. Glycobiology, 31(3), pp.288-306. |
| Corresponding Author | T N C Ramya |
| Contact | Department of Protein Science and Engineering, CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh 160036, India |