| Technique(s) used for Glycosylation Detection | Higher molecular mass detected on SDS-PAGE, Gel code glycoside detection kit (Pierce) |
| Technique(s) used for Glycosylated Residue(s) Detection | HPLC analysis after digestion with trypsin and aspartic N-peptidase (sequencing), site-directed mutagenesis, MALDI-TOF MS (matrix-assisted laser desorption/ionization time of flight mass spectrometry). |
| Protein Glycosylation- Implication | Glycosylation regulates the induction specificity of the immune responses (such as H2O2 generation) to flagellin. It prevents recognition of the flagellin epitope (of virulent strain K1) in rice. K1 flagellin without glycans induces a strong immune response in cultured rice cells. |
| Glycan Information |
| Glycan Annotation | The sites are glycosylated by an approximately 540 Da glycan that may be a derivative of the glycan of P. syringae pv. tabaci. |
| Technique(s) used for Glycan Identification | Mass spectroscopy |
| Literature |
| Year of Identification | 2000 |
| Year of Identification Month Wise | 2000.10.13 |
| Year of Validation | 2011 |
| Reference | Hirai, H., Takai, R., Iwano, M., Nakai, M., Kondo, M., Takayama, S., Isogai, A. and Che, F.S. (2011) Glycosylation regulates the specific induction of rice immune responses by Acidovorax avenae flagellin. J Biol Chem. ahead of print [PMID: 21628471] |
| Author | Hirai, H., Takai, R., Iwano, M., Nakai, M., Kondo, M., Takayama, S., Isogai, A. and Che, F.S. |
| Research Group | Graduate School of Bio-Science, Nagahama Institute of Bio-Science and Technology, Nagahama, Shiga 526-0829, Japan |
| Corresponding Author | Che, F.S. |
| Contact | Graduate School of Bio-Science, Nagahama Institute of Bio-Science and Technology, Nagahama, Shiga 526-0829, Japan |
| Reference | Che, F.S., Nakajima, Y., Tanaka, N., Iwano, M., Yoshida, T., Takayama, S., Kadota, I. and Isogai, A. (2000) Flagellin from an incompatible strain of Pseudomonas avenae induces a resistance response in cultured rice cells. J Biol Chem, 275, 32347-32356. [PubMed: 10922369] |
| Author | Che, F.S., Nakajima, Y., Tanaka, N., Iwano, M., Yoshida, T., Takayama, S., Kadota, I. and Isogai, A. |
| Research Group | Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5, Takayama Ikoma, Nara 630-0101, Japan |
| Corresponding Author | Isogai, A. |
| Contact | Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5, Takayama Ikoma, Nara 630-0101, Japan |
