| Technique(s) used for Glycosylation Detection | Mass shift on SDS-PAGE after deglycosylation with anhydrous hydrogen fluoride. |
| Technique(s) used for Glycosylated Residue(s) Detection | Glycopeptide sequencing |
| Glycan Information |
| Glycan Annotation | Linkage: Glc-Asn.
Sulfated oligosaccharides that resemble those of the cell-suface glycoprotein in the bacterium are present. Glucose and glucuronic acid are the constituents of the glycans which are of the type GlcA-(1?4)-GlcA-(1?4)-GlcA-(1?4)-Glc. |
| Technique(s) used for Glycan Identification | GLC-MS (gas liquid chromatography-mass spectrometry) after perfluoropropionylation. |
| Literature |
| Year of Identification | 1985 |
| Year of Identification Month Wise | 1985.12 |
| Year of Validation | 1989 |
| Reference | Cohen-Krausz, S. and Trachtenberg, S. (2002) The structure of the archeabacterial flagellar filament of the extreme halophile Halobacterium salinarum R1M1 and its relation to eubacterial flagellar filaments and type IV pili. J Mol Biol, 321, 383-395. [PubMed: 12162953] |
| Author | Cohen-Krausz, S. and Trachtenberg, S. |
| Research Group | Department of Membrane and Ultrastructure Research, Hebrew University of Jerusalem-Hadassah Medical School, P.O. Box 12272, 91120, Jerusalem, Israel. |
| Corresponding Author | Trachtenberg, S. |
| Contact | Department of Membrane and Ultrastructure Research, Hebrew University of Jerusalem-Hadassah Medical School, P.O. Box 12272, 91120, Jerusalem, Israel. |
| Reference | Gerl, L., Deutzmann, R. and Sumper, M. (1989) Halobacterial flagellins are encoded by a multigene family. Identification of all five gene products. FEBS Lett, 244, 137-140. [PubMed: 2924901] |
| Author | Gerl, L., Deutzmann, R. and Sumper, M. |
| Research Group | Chair of Biochemistry, University of Regensburg, FRG. |
| Corresponding Author | Sumper, M |
| Contact | Chair of Biochemistry, University of Regensburg, FRG. |
| Reference | Gerl, L. and Sumper, M. (1988) Halobacterial flagellins are encoded by a multigene family. Characterization of five flagellin genes. J Biol Chem, 263, 13246-13251. [PubMed: 3417656] |
| Author | Gerl, L. Sumper, M. |
| Research Group | Institute of Biochemistry, Genetics and Microbiology, University of Regensburg, Federal Republic of Germany. |
| Corresponding Author | Sumper, M. |
| Contact | Institute of Biochemistry, Genetics and Microbiology, University of Regensburg, Federal Republic of Germany. |
| Reference | Wieland, F., Paul, G. and Sumper, M. (1985) Halobacterial flagellins are sulfated glycoproteins. J Biol Chem, 260, 15180-15185. [PubMed: 3934156] |
| Author | Wieland, F., Paul, G. and Sumper, M. |
| Research Group | Institute of Biochemistry, Genetics and Microbiology, University of Regensburg, Universitatsstraße 31, 8400 Regensburg, Federal Republic of Germany |
| Corresponding Author | Sumper, M. |
| Contact | Institute of Biochemistry, Genetics and Microbiology, University of Regensburg, Universitatsstraße 31, 8400 Regensburg, Federal Republic of Germany |
