ProGP30 (Flagellin B1)

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ProGP ID ProGP30 (Flagellin B1)
Validation Status Characterized
Organism Information
Organism NameHalobacterium salinarum (halobium) R1M1/NRC-1
Domain Archaea
Classification Family: Halobacteriaceae
Order: Halobacteriales
Class: Halobacteria or Halomebacteria
Division or phylum: "Euryarchaeota"
Taxonomic ID (NCBI) 64091
Genome Information
GenBank AE004437.1
EMBL AE004437
Gene Information
Gene NameflaB1 (VNG_0960G)
NCBI Gene ID 1447685
GenBank Gene Sequence NC_002607.1
Protein Information
Protein NameFlagellin B1
UniProtKB/SwissProt ID P61116
NCBI RefSeq NP_279903.1
EMBL-CDSAAG19383.1
UniProtKB Sequence >sp|P61116|FLAB1_HALSA Flagellin B1 OS=Halobacterium salinarium (strain ATCC 700922 / JCM 11081 / NRC-1) GN=flaB1 PE=3 SV=1 MFEFITDEDERGQVGIGTLIVFIAMVLVAAIAAGVLINTAGYLQSKGSATGEEASAQVSN RINIVSAYGNVNNEKVDYVNLTVRQAAGADNINLTKSTIQWIGPDRATTLTYSSNSPSSL GENFTTESIKGSSADVLVDQSDRIKVIMYASGVSSNLGAGDEVQLTVTTQYGSKTTYWAQ VPESLKDKNAVTL
Sequence length 193 AA
Subcellular LocationFlagellum
Function Flagellin is the structural subunit of the flagellar filaments.
Glycosylation Status
Glycosylation Type N- (Asn) linked
Experimentally Validated Glycosite(s) in Full Length ProteinN93
Experimentally Validated Glycosite(s ) in Mature ProteinN93
Glycosite(s) Annotated Protein Sequence >sp|P61116|FLAB1_HALSA Flagellin B1 OS=Halobacterium salinarium (strain ATCC 700922 / JCM 11081 / NRC-1) GN=flaB1 PE=3 SV=1 MFEFITDEDERGQVGIGTLIVFIAMVLVAAIAAGVLINTAGYLQSKGSATGEEASAQVSN RINIVSAYGNVNNEKVDYVNLTVRQAAGADNIN*(93)LTKSTIQWIGPDRATTLTYSSNSPSSL GENFTTESIKGSSADVLVDQSDRIKVIMYASGVSSNLGAGDEVQLTVTTQYGSKTTYWAQ VPESLKDKNAVTL
Sequence Around Glycosites (21 AA) VRQAAGADNINLTKSTIQWIG
Technique(s) used for Glycosylation DetectionMass shift on SDS-PAGE after deglycosylation with anhydrous hydrogen fluoride.
Technique(s) used for Glycosylated Residue(s) Detection Glycopeptide sequencing
Glycan Information
Glycan Annotation Linkage: Glc-Asn.
Sulfated oligosaccharides that resemble those of the cell-suface glycoprotein in the bacterium are present. Glucose and glucuronic acid are the constituents of the glycans which are of the type GlcA-(1→4)-GlcA-(1→4)-GlcA-(1→4)-Glc.
BCSDB ID 136320
GlyTouCan G81788YA
Technique(s) used for Glycan Identification GLC-MS (gas liquid chromatography-mass spectrometry) after perfluoropropionylation.
Literature
Year of Identification1985
Year of Identification Month Wise1985.12
Year of Validation 1988
ReferenceCohen-Krausz, S. and Trachtenberg, S., 2002. The structure of the archeabacterial flagellar filament of the extreme halophile Halobacterium salinarum R1M1 and its relation to eubacterial flagellar filaments and type IV pili. Journal of molecular biology, 321(3), pp.383-395.
Corresponding Author Shlomo Trachtenberg
ContactDepartment of Membrane and Ultrastructure Research, Hebrew University of Jerusalem-Hadassah Medical School, P.O. Box 12272, 91120, Jerusalem, Israel.
ReferenceGerl, L., Deutzmann, R. and Sumper, M., 1989. Halobacterial flagellins are encoded by a multigene family Identification of all five gene products. FEBS letters, 244(1), pp.137-140.
Corresponding Author Manfred Sumper
ContactInstitute of Biochemistry, Genetics and Microbiology, University of Regensburg, Federal Republic of Germany.
ReferenceGerl, L. and Sumper, M., 1988. Halobacterial flagellins are encoded by a multigene family. Characterization of five flagellin genes. Journal of Biological Chemistry, 263(26), pp.13246-13251.
Corresponding Author Manfred Sumper
ContactInstitute of Biochemistry, Genetics and Microbiology, University of Regensburg, Federal Republic of Germany.
ReferenceWieland, F., Paul, G. and Sumper, M., 1985. Halobacterial flagellins are sulfated glycoproteins. Journal of Biological Chemistry, 260(28), pp.15180-15185.
Corresponding Author Felix Wieland
ContactInstitute of Biochemistry, Genetics and Microbiology, University of Regensburg, Universitatsstraße 31, 8400 Regensburg, Federal Republic of Germany
ReferenceVershinin, Z., Zaretsky, M., Guan, Z. and Eichler, J., 2021. Revisiting N-glycosylation in Halobacterium salinarum: Characterizing a dolichol phosphate-and glycoprotein-bound tetrasaccharide. Glycobiology.
Corresponding Author Jerry Eichler
ContactDepartment of Life Sciences, Ben Gurion University of the Negev, PO Box 653, Beersheva 84105, Israel.
ReferenceCohen-Krausz, S. and Trachtenberg, S. (2002) The structure of the archeabacterial flagellar filament of the extreme halophile Halobacterium salinarum R1M1 and its relation to eubacterial flagellar filaments and type IV pili. J Mol Biol, 321, 383-395. [PubMed: 12162953]
Author Cohen-Krausz, S. and Trachtenberg, S.
Research GroupDepartment of Membrane and Ultrastructure Research, Hebrew University of Jerusalem-Hadassah Medical School, P.O. Box 12272, 91120, Jerusalem, Israel.
Corresponding Author Trachtenberg, S.
ContactDepartment of Membrane and Ultrastructure Research, Hebrew University of Jerusalem-Hadassah Medical School, P.O. Box 12272, 91120, Jerusalem, Israel.
ReferenceGerl, L., Deutzmann, R. and Sumper, M. (1989) Halobacterial flagellins are encoded by a multigene family. Identification of all five gene products. FEBS Lett, 244, 137-140. [PubMed: 2924901]
AuthorGerl, L., Deutzmann, R. and Sumper, M.
Research GroupChair of Biochemistry, University of Regensburg, FRG.
Corresponding Author Sumper, M
ContactChair of Biochemistry, University of Regensburg, FRG.
ReferenceGerl, L. and Sumper, M. (1988) Halobacterial flagellins are encoded by a multigene family. Characterization of five flagellin genes. J Biol Chem, 263, 13246-13251. [PubMed: 3417656]
AuthorGerl, L. Sumper, M.
Research GroupInstitute of Biochemistry, Genetics and Microbiology, University of Regensburg, Federal Republic of Germany.
Corresponding Author Sumper, M.
ContactInstitute of Biochemistry, Genetics and Microbiology, University of Regensburg, Federal Republic of Germany.
ReferenceWieland, F., Paul, G. and Sumper, M. (1985) Halobacterial flagellins are sulfated glycoproteins. J Biol Chem, 260, 15180-15185. [PubMed: 3934156]
AuthorWieland, F., Paul, G. and Sumper, M.
Research GroupInstitute of Biochemistry, Genetics and Microbiology, University of Regensburg, Universitatsstraße 31, 8400 Regensburg, Federal Republic of Germany
Corresponding Author Sumper, M.
ContactInstitute of Biochemistry, Genetics and Microbiology, University of Regensburg, Universitatsstraße 31, 8400 Regensburg, Federal Republic of Germany