ProGP30 (Flagellin B1)
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| ProGP ID | ProGP30 (Flagellin B1) |
| Validation Status | Characterized |
| Organism Information | |
| Organism Name | Halobacterium salinarum (halobium) R1M1/NRC-1 |
| Domain | Archaea |
| Classification | Family: Halobacteriaceae Order: Halobacteriales Class: Halobacteria or Halomebacteria Division or phylum: "Euryarchaeota" |
| Taxonomic ID (NCBI) | 64091 |
| Genome Information | |
| GenBank | AE004437.1 |
| EMBL | AE004437 |
| Gene Information | |
| Gene Name | flaB1 (VNG_0960G) |
| NCBI Gene ID | 1447685 |
| GenBank Gene Sequence | NC_002607.1 |
| Protein Information | |
| Protein Name | Flagellin B1 |
| UniProtKB/SwissProt ID | P61116 |
| NCBI RefSeq | NP_279903.1 |
| EMBL-CDS | AAG19383.1 |
| UniProtKB Sequence | >sp|P61116|FLAB1_HALSA Flagellin B1 OS=Halobacterium salinarium (strain ATCC 700922 / JCM 11081 / NRC-1) GN=flaB1 PE=3 SV=1 MFEFITDEDERGQVGIGTLIVFIAMVLVAAIAAGVLINTAGYLQSKGSATGEEASAQVSN RINIVSAYGNVNNEKVDYVNLTVRQAAGADNINLTKSTIQWIGPDRATTLTYSSNSPSSL GENFTTESIKGSSADVLVDQSDRIKVIMYASGVSSNLGAGDEVQLTVTTQYGSKTTYWAQ VPESLKDKNAVTL |
| Sequence length | 193 AA |
| Subcellular Location | Flagellum |
| Function | Flagellin is the structural subunit of the flagellar filaments. |
| Glycosylation Status | |
| Glycosylation Type | N- (Asn) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | N93 |
| Experimentally Validated Glycosite(s ) in Mature Protein | N93 |
| Glycosite(s) Annotated Protein Sequence | >sp|P61116|FLAB1_HALSA Flagellin B1 OS=Halobacterium salinarium (strain ATCC 700922 / JCM 11081 / NRC-1) GN=flaB1 PE=3 SV=1 MFEFITDEDERGQVGIGTLIVFIAMVLVAAIAAGVLINTAGYLQSKGSATGEEASAQVSN RINIVSAYGNVNNEKVDYVNLTVRQAAGADNIN*(93)LTKSTIQWIGPDRATTLTYSSNSPSSL GENFTTESIKGSSADVLVDQSDRIKVIMYASGVSSNLGAGDEVQLTVTTQYGSKTTYWAQ VPESLKDKNAVTL |
| Sequence Around Glycosites (21 AA) | VRQAAGADNINLTKSTIQWIG |
| Technique(s) used for Glycosylation Detection | Mass shift on SDS-PAGE after deglycosylation with anhydrous hydrogen fluoride. |
| Technique(s) used for Glycosylated Residue(s) Detection | Glycopeptide sequencing |
| Glycan Information | |
| Glycan Annotation | Linkage: Glc-Asn. Sulfated oligosaccharides that resemble those of the cell-suface glycoprotein in the bacterium are present. Glucose and glucuronic acid are the constituents of the glycans which are of the type GlcA-(1→4)-GlcA-(1→4)-GlcA-(1→4)-Glc. |
| BCSDB ID | 136320 |
| GlyTouCan | G81788YA |
| Technique(s) used for Glycan Identification | GLC-MS (gas liquid chromatography-mass spectrometry) after perfluoropropionylation. |
| Literature | |
| Year of Identification | 1985 |
| Year of Identification Month Wise | 1985.12 |
| Year of Validation | 1988 |
| Reference | Cohen-Krausz, S. and Trachtenberg, S. (2002) The structure of the archeabacterial flagellar filament of the extreme halophile Halobacterium salinarum R1M1 and its relation to eubacterial flagellar filaments and type IV pili. J Mol Biol, 321, 383-395. [PubMed: 12162953] |
| Author | Cohen-Krausz, S. and Trachtenberg, S. |
| Research Group | Department of Membrane and Ultrastructure Research, Hebrew University of Jerusalem-Hadassah Medical School, P.O. Box 12272, 91120, Jerusalem, Israel. |
| Corresponding Author | Trachtenberg, S. |
| Contact | Department of Membrane and Ultrastructure Research, Hebrew University of Jerusalem-Hadassah Medical School, P.O. Box 12272, 91120, Jerusalem, Israel. |
| Reference | Gerl, L., Deutzmann, R. and Sumper, M. (1989) Halobacterial flagellins are encoded by a multigene family. Identification of all five gene products. FEBS Lett, 244, 137-140. [PubMed: 2924901] |
| Author | Gerl, L., Deutzmann, R. and Sumper, M. |
| Research Group | Chair of Biochemistry, University of Regensburg, FRG. |
| Corresponding Author | Sumper, M |
| Contact | Chair of Biochemistry, University of Regensburg, FRG. |
| Reference | Gerl, L. and Sumper, M. (1988) Halobacterial flagellins are encoded by a multigene family. Characterization of five flagellin genes. J Biol Chem, 263, 13246-13251. [PubMed: 3417656] |
| Author | Gerl, L. Sumper, M. |
| Research Group | Institute of Biochemistry, Genetics and Microbiology, University of Regensburg, Federal Republic of Germany. |
| Corresponding Author | Sumper, M. |
| Contact | Institute of Biochemistry, Genetics and Microbiology, University of Regensburg, Federal Republic of Germany. |
| Reference | Wieland, F., Paul, G. and Sumper, M. (1985) Halobacterial flagellins are sulfated glycoproteins. J Biol Chem, 260, 15180-15185. [PubMed: 3934156] |
| Author | Wieland, F., Paul, G. and Sumper, M. |
| Research Group | Institute of Biochemistry, Genetics and Microbiology, University of Regensburg, Universitatsstraße 31, 8400 Regensburg, Federal Republic of Germany |
| Corresponding Author | Sumper, M. |
| Contact | Institute of Biochemistry, Genetics and Microbiology, University of Regensburg, Universitatsstraße 31, 8400 Regensburg, Federal Republic of Germany |