ProGP343 (PilA (FTH_0384))
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| ProGP ID | ProGP343 (PilA (FTH_0384)) |
| Validation Status | Characterized |
| Organism Information | |
| Organism Name | Francisella tularensis ssp. holarctica strain FSC200 |
| Domain | Bacteria |
| Classification | Phylum : Proteobacteria Class : Gammaproteobacteria Orders : Thiotrichales Family : Francisellaceae Genus : Francisella Species : tularensis Subspecies : holarctica Strain : FSC 200 |
| Taxonomic ID (NCBI) | 351581 |
| Genome Information | |
| GenBank | CP003862.1 |
| EMBL | CP003862.1 |
| Gene Information | |
| Gene Name | PilA (FTH_0384) |
| GenBank Gene Sequence | NC_008369.1 |
| Protein Information | |
| Protein Name | PilA (FTH_0384) |
| UniProtKB/SwissProt ID | A0SP58 |
| NCBI RefSeq | WP_011648587.1 |
| EMBL-CDS | AAX14621.1 |
| UniProtKB Sequence | >tr|A0SP58|A0SP58_FRATU PilE1 OS=Francisella tularensis subsp. holarctica GN=pilA PE=3 SV=1 MKKKMQKGFSLVELMVVIAIIAILAAVAIPMYSNYTTRAQLGSDLSALGGAKAIVAEKIA NNNGDASQVTILQANAAANGLPSGASVAAGTISYPSTVSGATIQLAPTVSSGAITWTCNI SGVSASQVPSNCNAI |
| Sequence length | 135 AA |
| Subcellular Location | Membrane |
| Function | Type IV pili fiber building block protein |
| Protein Structure | |
| Protein Additional Information | This protein shares 52.5% similarity with pilE1-encoded pilin (FMM1_NEIGO) of N. gonorrheae, which is modified with Gal 1-3GlcNAc at Ser70. |
| Glycosylation Status | |
| Glycosylation Type | O- (Ser/Thr) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | One of either S103 or S102 |
| Glycosite(s) Annotated Protein Sequence | >tr|A0SP58|A0SP58_FRATU PilE1 OS=Francisella tularensis subsp. holarctica GN=pilA PE=3 SV=1 MKKKMQKGFSLVELMVVIAIIAILAAVAIPMYSNYTTRAQLGSDLSALGGAKAIVAEKIA NNNGDASQVTILQANAAANGLPSGASVAAGTISYPSTVSGATIQLAPTVS*(102)S*(103)GAITWTCNI SGVSASQVPSNCNAI |
| Sequence Around Glycosites (21 AA) | GATIQLAPTVSSGAITWTCNI ATIQLAPTVSSGAITWTCNIS |
| Technique(s) used for Glycosylation Detection | Hydrazide labeling and lectin affinity chromatography. |
| Technique(s) used for Glycosylated Residue(s) Detection | MSMS (Ion trap with electron transfer dissociation (ETD)) |
| Protein Glycosylation- Implication | Pilin glycosylation has been shown to play a role in host-cell adhesion and virulence. |
| Glycan Information | |
| Glycan Annotation | Pentasaccharide (HexNAc-Hex-Hex-HexNAc-HexNAc) is linked through its terminal HexNAc residue to a 162.111-Da moiety via a phosphate bridge. |
| Technique(s) used for Glycan Identification | MS (ion trap with tandemMS CID and ETD) |
| Protein Glycosylation linked (PGL) gene(s) | |
| OST Gene Name | PglA |
| OST ProGT ID | ProGT50 |
| Literature | |
| Year of Identification | 2010 |
| Year of Identification Month Wise | 2010.04 |
| Year of Validation | 2011 |
| Reference | Balonova, L., Hernychova, L., Mann, B.F., Link, M., Bilkova, Z., Novotny, M.V. and Stulik, J., 2010. Multimethodological approach to identification of glycoproteins from the proteome of Francisella tularensis, an intracellular microorganism. Journal of proteome research, 9(4), pp.1995-2005. |
| Corresponding Author | Lenka Hernychova |
| Contact | Institute of Molecular Pathology, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic |
| Reference | Egge-Jacobsen, W., Salomonsson, E.N., Aas, F.E., Forslund, A.L., Winther-Larsen, H.C., Maier, J., Macellaro, A., Kuoppa, K., Oyston, P.C., Titball, R.W. and Thomas, R.M., 2011. O-linked glycosylation of the PilA pilin protein of Francisella tularensis: identification of the endogenous protein-targeting oligosaccharyltransferase and characterization of the native oligosaccharide. Journal of bacteriology, 193(19), pp.5487-5497. |
| Corresponding Author | Wolfgang Egge-Jacobsen Michael Koomey |
| Contact | a.Department of Molecular Biosciences and b.Center for Molecular Biology and Neuroscience, University of Oslo, Oslo 0316, Norway |
| Reference | Balonova, L., Mann, B.F., Cerveny, L., Alley, W.R., Chovancova, E., Forslund, A.L., Salomonsson, E.N., Forsberg, Å., Damborsky, J., Novotny, M.V. and Hernychova, L., 2012. Characterization of protein glycosylation in Francisella tularensis subsp. holarctica: identification of a novel glycosylated lipoprotein required for virulence. Molecular & Cellular Proteomics, 11(7), pp.M111-015016. |
| Corresponding Author | Lenka Hernychova |
| Contact | Institute of Molecular Pathology, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic |