ProGP380 (Putative periplasmic protein)

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ProGP ID ProGP380 (Putative periplasmic protein)
Validation Status Characterized
Organism Information
Organism NameCampylobacter jejuni HB93-13
Domain Bacteria
Classification Phylum : Proteobacteria
Class : Epsilonproteobacteria
Orders : Campylobacterales
Family : Campylobacteraceae
Genus : Campylobacter
Species : jejuni
Subspecies : jejuni
Strain : HB93-13
Taxonomic ID (NCBI) 192222
Genome Information
GenBank NC_002163.1
EMBL AL111168
Organism Additional Information Campylobacter jejuni is a microaerophilic, Gram-negative, human pathogen that is the major cause of bacterial food-borne diarrhoea (gastroenteritis). It is most frequently responsible for a form of post-infection neuromuscular paralysis known as Guillain Barre' syndrome. It also leads to an immunoproliferative small intestine disease that is a rare malignant lymphoma of the intestine. Motility is essential for pathogenicity.
Gene Information
Gene Namecj0694
NCBI Gene ID 905013
GenBank Gene Sequence NC_002163.1.
Protein Information
Protein NamePutative periplasmic protein
UniProtKB/SwissProt ID Q0PAI5
NCBI RefSeq WP_002852128.1
EMBL-CDSCAL34831.1
UniProtKB Sequence >tr|Q0PAI5|Q0PAI5_CAMJE Putative periplasmic protein OS=Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) GN=Cj0694 PE=4 SV=1 MLTWMQHHKKYLVVTIWVSTIAFVGAGFLGWGAYDFNLNRSSSVATVGNEKIGFSEFDTR YRQILSYYNQISNGALTPENAEQLGIKNIALSSLVEDKLLLNFAKDLGIGVNENQILQKL ANTREFQDPTGDFNKTIYYELLNANNLAPKDYETQLANEVITDKLNQIFNIPSKDEELKM LASSYFMQDALSIAKIDYDKKNIKINEEDLKKLWNEHKEDYKTKKIYEISTYFLPVSNEK IDDKELEKFYNQDENKLKYKDFAGKVMDFQSAKNEVAKDYALMQLKNVANAKFLDLKNGK DNFQKDQNISESDVYYPIDLLNKAKNGDVLRPAPYNNGYIIVKLNKVDPIRNKTFEEARE EVLPMYLSEQARKNLEEKAKNSLVNFKGDDIGFVSRDSSRESVKVSDKILNDSEFAYFLM NVFNTDQNSSYVVINDNKAILYKINKQKLDMNSDKFEQYKTMLEQNLQNLKANELKQELV DELKKIYPIKIYYKGN
Sequence length 496 AA
Glycosylation Status
Glycosylation Type N- (Asn) linked
Experimentally Validated Glycosite(s) in Full Length ProteinN308, N134 and N308
Glycosite(s) Annotated Protein Sequence >tr|Q0PAI5|Q0PAI5_CAMJE Putative periplasmic protein OS=Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) GN=Cj0694 PE=4 SV=1 MLTWMQHHKKYLVVTIWVSTIAFVGAGFLGWGAYDFNLNRSSSVATVGNEKIGFSEFDTR YRQILSYYNQISNGALTPENAEQLGIKNIALSSLVEDKLLLNFAKDLGIGVNENQILQKL ANTREFQDPTGDFN*(134)KTIYYELLNANNLAPKDYETQLANEVITDKLNQIFNIPSKDEELKM LASSYFMQDALSIAKIDYDKKNIKINEEDLKKLWNEHKEDYKTKKIYEISTYFLPVSNEK IDDKELEKFYNQDENKLKYKDFAGKVMDFQSAKNEVAKDYALMQLKNVANAKFLDLKNGK DNFQKDQN*(308)ISESDVYYPIDLLNKAKNGDVLRPAPYNNGYIIVKLNKVDPIRNKTFEEARE EVLPMYLSEQARKNLEEKAKNSLVNFKGDDIGFVSRDSSRESVKVSDKILNDSEFAYFLM NVFNTDQNSSYVVINDNKAILYKINKQKLDMNSDKFEQYKTMLEQNLQNLKANELKQELV DELKKIYPIKIYYKGN
Sequence Around Glycosites (21 AA) REFQDPTGDFNKTIYYELLNA
NGKDNFQKDQNISESDVYYPI
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Technique(s) used for Glycosylation DetectionSoybean Lectin Affinity Enrichment and ZIC-HILIC enrichment
Technique(s) used for Glycosylated Residue(s) Detection Reversed Phase LC-Tandem CID/HCD-MS, CID/ETD-MS and ESI (CID)-MS/MS after enrichment using graphite microcolumns
Glycan Information
Glycan Annotation Heptasaccharide GalNAc- α1,4-GalNAc- α1,4-(Glcβ1,3)-GalNAc- α1,4-GalNAc- α1,4-GalNAc- α1,3-Bac-β1 where Bac is bacillosamine (2,4-diacetamido-2,4,6-trideoxyglucopyranose)
BCSDB ID 20059
GlyTouCan G38348FF
Technique(s) used for Glycan Identification Reversed Phase LC-Tandem CID/HCD-MS
Literature
Year of Identification2011
Year of Identification Month Wise2011.2.10
Year of Validation 2011
ReferenceScott, N.E., Parker, B.L., Connolly, A.M., Paulech, J., Edwards, A.V., Crossett, B., Falconer, L., Kolarich, D., Djordjevic, S.P., Højrup, P. and Packer, N.H., 2011. Simultaneous glycan-peptide characterization using hydrophilic interaction chromatography and parallel fragmentation by CID, higher energy collisional dissociation, and electron transfer dissociation MS applied to the N-linked glycoproteome of Campylobacter jejuni. Molecular & cellular proteomics, 10(2), pp.S1-S18.
Corresponding Author Stuart J Cordwell
ContactSchool of Molecular and Microbial Biosciences, University of Sydney, Sydney, Australia