ProGP383 (Uncharacterized metallophosphoesterase)
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| ProGP ID | ProGP383 (Uncharacterized metallophosphoesterase) |
| Validation Status | Characterized |
| Organism Information | |
| Organism Name | Campylobacter jejuni HB93-13 |
| Domain | Bacteria |
| Classification | Family: Campylobacteraceae Order: "Campylobacterales" Class: "Epsilonproteobacteria" Division or phylum: "Proteobacteria" |
| Taxonomic ID (NCBI) | 192222 |
| Genome Information | |
| GenBank | NC_002163.1. |
| EMBL | AL111168 |
| Organism Additional Information | Campylobacter jejuni is a microaerophilic, Gram-negative, human pathogen that is the major cause of bacterial food-borne diarrhoea (gastroenteritis). It is most frequently responsible for a form of post-infection neuromuscular paralysis known as Guillain Barre' syndrome. It also leads to an immunoproliferative small intestine disease that is a rare malignant lymphoma of the intestine. Motility is essential for pathogenicity. |
| Gene Information | |
| Gene Name | cj0846 |
| NCBI Gene ID | 905145 |
| GenBank Gene Sequence | NC_002163.1. |
| Protein Information | |
| Protein Name | Uncharacterized metallophosphoesterase |
| UniProtKB/SwissProt ID | Q9PP77 |
| NCBI RefSeq | WP_002871823.1 |
| EMBL-CDS | CAL34974.1. |
| UniProtKB Sequence | >sp|Q9PP77|Y846_CAMJE Uncharacterized metallophosphoesterase Cj0846 OS=Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) GN=Cj0846 PE=3 SV=1 MIFLIFSFIVLLIFGLANVYIYKRLIKKITLFKYFYKIFSFIFIVLFLAQAVFLIFRRDE YLSDTWYEILAMFYAPTYCLFFMTLAWDFVKLILALMGKRDKTYNLILRLIFELSLIVLS VFLIYASINNALKTPEVKSVDVEIPNLKKDLKIVMLTDIHLGKNLHENFLDKLITKVNLQ SPDMVVIVGDLIDTNPKDLKNYISKLNDFNSTYGTFYALGNHEYYHGINEVLDLLRKHTN MKILVNQNLDLGFIDIAGLGDLAGLDRGLYAPDLARIKVDLNTSKASILLTHQPKTALLY DLSDFDLVLSGHTHGGQIFPFMFLVKLQQGFVHGLYDLGEKTKLYVSSGAGFWGPSLRVF APSEIVILNLKGKK |
| Sequence length | 374 AA |
| Glycosylation Status | |
| Glycosylation Type | N- (Asn) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | N282 |
| Glycosite(s) Annotated Protein Sequence | >sp|Q9PP77|Y846_CAMJE Uncharacterized metallophosphoesterase Cj0846 OS=Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) GN=Cj0846 PE=3 SV=1 MIFLIFSFIVLLIFGLANVYIYKRLIKKITLFKYFYKIFSFIFIVLFLAQAVFLIFRRDE YLSDTWYEILAMFYAPTYCLFFMTLAWDFVKLILALMGKRDKTYNLILRLIFELSLIVLS VFLIYASINNALKTPEVKSVDVEIPNLKKDLKIVMLTDIHLGKNLHENFLDKLITKVNLQ SPDMVVIVGDLIDTNPKDLKNYISKLNDFNSTYGTFYALGNHEYYHGINEVLDLLRKHTN MKILVNQNLDLGFIDIAGLGDLAGLDRGLYAPDLARIKVDLN*(282)TSKASILLTHQPKTALLY DLSDFDLVLSGHTHGGQIFPFMFLVKLQQGFVHGLYDLGEKTKLYVSSGAGFWGPSLRVF APSEIVILNLKGKK |
| Sequence Around Glycosites (21 AA) | PDLARIKVDLNTSKASILLTH |
| Technique(s) used for Glycosylation Detection | ZIC-HILIC enrichment |
| Technique(s) used for Glycosylated Residue(s) Detection | Reversed Phase LC-Tandem CID/HCD-MS |
| Glycan Information | |
| Glycan Annotation | Heptasaccharide GalNAc- α1,4-GalNAc- α1,4-(Glc β1,3)-GalNAc- α1,4-GalNAc- α1,4-GalNAc- α1,3-Bac- β1 where Bac is bacillosamine (2,4-diacetamido-2,4,6-trideoxyglucopyranose) |
| BCSDB ID | 20059 |
| GlyTouCan | G38348FF |
| Technique(s) used for Glycan Identification | Reversed Phase LC-Tandem CID/HCD-MS |
| Literature | |
| Year of Identification | 2011 |
| Year of Identification Month Wise | 2011.2.10 |
| Year of Validation | 2011 |
| Reference | Scott, N.E., Parker, B.L., Connolly, A.M., Paulech, J., Edwards, A.V., Crossett, B., Falconer, L., Kolarich, D., Djordjevic, S.P., Højrup, P. and Packer, N.H., 2011. Simultaneous glycan-peptide characterization using hydrophilic interaction chromatography and parallel fragmentation by CID, higher energy collisional dissociation, and electron transfer dissociation MS applied to the N-linked glycoproteome of Campylobacter jejuni. Molecular & cellular proteomics, 10(2), pp.S1-S18. |
| Corresponding Author | Stuart J Cordwell |
| Contact | School of Molecular and Microbial Biosciences, University of Sydney, Sydney, Australia |
| Reference | Scott NE, Parker BL, Connolly AM, Paulech J, Edwards AV, Crossett B, Falconer L, Kolarich D, Djordjevic SP, Højrup P, Packer NH, Larsen MR, Cordwell SJ. (2011) Simultaneous glycan-peptide characterization using hydrophilic interaction chromatography and parallel fragmentation by CID, higher energy collisional dissociation, and electron transfer dissociation MS applied to the N-linked glycoproteome of Campylobacter jejuni. Mol Cell Proteomics., 10(2):M000031-MCP201. [PubMed: 20360033] |
| Author | Scott NE, Parker BL, Connolly AM, Paulech J, Edwards AV, Crossett B, Falconer L, Kolarich D, Djordjevic SP, Højrup P, Packer NH, Larsen MR, Cordwell SJ |
| Research Group | School of Molecular and Microbial Biosciences, University of Sydney, Sydney, Australia |
| Corresponding Author | Cordwell SJ |
| Contact | School of Molecular and Microbial Biosciences, University of Sydney, Sydney, Australia |