ProGP402 (Glycocin F)
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| ProGP ID | ProGP402 (Glycocin F) |
| Validation Status | Characterized |
| Organism Information | |
| Organism Name | Lactobacillus plantarum KW30 |
| Domain | Bacteria |
| Classification | Family: Lactobacillaceae Order: Lactobacillales Class: Bacilli (or Firmibacteria) Division or phylum: "Firmicutes" |
| Taxonomic ID (NCBI) | 1590 |
| Genome Information | |
| GenBank | GU552553 |
| EMBL | GU552553 |
| Gene Information | |
| Gene Name | gccF |
| Protein Information | |
| Protein Name | Glycocin F |
| UniProtKB/SwissProt ID | E9K9Z1 |
| EMBL-CDS | ADV57366.1 |
| UniProtKB Sequence | >tr|E9K9Z1|E9K9Z1_LACPL Prebacteriocin glycocin F OS=Lactobacillus plantarum GN=gccF PE=4 SV=1 MSKLVKTLTISEISKAQNNGGKPAWCWYTLAMCGAGYDSGTCDYMYSHCFGIKHHSSGSS SYHC |
| Sequence length | 64 AA |
| Subcellular Location | Secreted |
| Function | Glycocin F is a bacteriocin that possesses bacteriostatic activity. This activity is reversed by free N-acetylglucosamine. |
| Protein Structure | |
| PDB ID | 2KUY |
| Glycosylation Status | |
| Glycosylation Type | O- (Ser) and S- (Cys) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | (Signal peptide: 1-21) S39, C64 |
| Experimentally Validated Glycosite(s ) in Mature Protein | S18, C43 |
| Glycosite(s) Annotated Protein Sequence | >tr|E9K9Z1|E9K9Z1_LACPL Prebacteriocin glycocin F OS=Lactobacillus plantarum GN=gccF PE=4 SV=1 MSKLVKTLTISEISKAQNNGGKPAWCWYTLAMCGAGYDS*(39)GTCDYMYSHCFGIKHHSSGSS SYHC*(64) |
| Sequence Around Glycosites (21 AA) | TLAMCGAGYDSGTCDYMYSHC
HHSSGSSSYHC |
| Technique(s) used for Glycosylation Detection | Mass difference measured and accounted for by Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) with electron capture dissociation (ECD) |
| Technique(s) used for Glycosylated Residue(s) Detection | Edman sequencing and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) |
| Protein Glycosylation- Implication | O-linked N-acetylglucosamine is required for bacteriostatic activity. |
| Glycan Information | |
| Glycan Annotation | Linkages: ?-GlcNAc-Ser, HexNAc-Cys. N-Acetylglucosamine is ?-O-linked to Ser18 and N-acetylhexosamine is S-linked to C-terminal Cys43. |
| Technique(s) used for Glycan Identification | N-acetyl-?-D-glucosaminidase GcnA treatment. |
| Literature | |
| Year of Identification | 2011 |
| Year of Identification Month Wise | 2011.5.20 |
| Year of Validation | 2011 |
| Reference | Stepper, J., Shastri, S., Loo, T.S., Preston, J.C., Novak, P., Man, P., Moore, C.H., Havlicek, V., Patchett, M.L. and Norris, G.E. (2011) Cysteine S-glycosylation, a new post-translational modification found in glycopeptide bacteriocins. FEBS Lett, 585, 645-650. [PubMed: 21251913] |
| Author | Stepper, J., Shastri, S., Loo, T.S., Preston, J.C., Novak, P., Man, P., Moore, C.H., Havlicek, V., Patchett, M.L. and Norris, G.E. |
| Research Group | Institute of Molecular Biosciences, Massey University, Palmerston North, New Zealand. |
| Corresponding Author | Norris, G.E |
| Contact | Institute of Molecular Biosciences, Massey University, Palmerston North, New Zealand. |
| Reference | Venugopal, H., Edwards, P.J., Schwalbe, M., Claridge, J.K., Libich, D.S., Stepper, J., Loo, T., Patchett, M.L., Norris, G.E. and Pascal, S.M. (2011) Structural, dynamic, and chemical characterization of a novel S-glycosylated bacteriocin. Biochemistry, 50, 2748-2755. [PubMed: 21395300] |
| Author | Venugopal, H., Edwards, P.J., Schwalbe, M., Claridge, J.K., Libich, D.S., Stepper, J., Loo, T., Patchett, M.L., Norris, G.E. Pascal, S.M. |
| Research Group | Institute of Fundamental Sciences, Massey University, Palmerston North, New Zealand |
| Corresponding Author | Pascal, S.M. |
| Contact | Institute of Fundamental Sciences, Massey University, Palmerston North, New Zealand |