ProGP409 (FliC (Flagellin))
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| ProGP ID | ProGP409 (FliC (Flagellin)) |
| Validation Status | Characterized |
| Organism Information | |
| Organism Name | Burkholderia pseudomallei K96243 |
| Domain | Bacteria |
| Classification | Phylum : Proteobacteria Class : Betaproteobacteria Orders : Burkholderiales Family : Burkholderiaceae Genus : Burkholderia Species : pseudomallei Strain : K96243 |
| Taxonomic ID (NCBI) | 272560 |
| Genome Information | |
| GenBank | NC_006350 and NC_006351 |
| EMBL | BX571965.1 and BX571966.1 |
| Organism Additional Information | Burkholderia pseudomallei, a saprophyte classified as a potential biothreat agent, is responsible for causing melioidosis, an infectious disease in humans. |
| Gene Information | |
| Gene Name | FliC |
| GenBank Gene Sequence | NC_006350.1 |
| Protein Information | |
| Protein Name | FliC (Flagellin) |
| UniProtKB/SwissProt ID | H7C7G3 |
| NCBI RefSeq | WP_004198207.1 |
| EMBL-CDS | CAH37332.1 |
| UniProtKB Sequence | >tr|H7C7G3|H7C7G3_BURPS Flagellin OS=Burkholderia pseudomallei (strain K96243) GN=fliC PE=3 SV=1 MLGINSNINSLVAQQNLNGSQGALSQAITRLSSGKRINSAADDAAGLAIATRMQTQINGL NQGVSNANDGVSILQTASSGLTSLTNSLQRIRQLAVQASNGPLSASDASALQQEVAQQIS EVNRIASQTNYNGKNILDGSAGTLSFQVGANVGQTVSVDLTQSMSAAKIGGGMVQTGQTL GTIKVAIDSSGAAWSSGSTGQETTQINVVSDGKGGFTFTDQNNQALSSTAVTAVFGSSTA GTGTAASPSFQTLALSTSATSALSATDQANATAMVAQINAVNKPQTVSNLDISTQTGAYQ AMVSIDNALATVNNLQATLGAAQNRFTAIATTQQAGSNNLAQAQSQIQSADFAQETANLS RAQVLQQAGISVLAQANSLPQQVLKLLQ |
| Sequence length | 388 AA |
| Subcellular Location | Flagellum |
| Function | Motility |
| Glycosylation Status | |
| Glycosylation Type | O- (Ser) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | S198 |
| Glycosite(s) Annotated Protein Sequence | >tr|H7C7G3|H7C7G3_BURPS Flagellin OS=Burkholderia pseudomallei (strain K96243) GN=fliC PE=3 SV=1 MLGINSNINSLVAQQNLNGSQGALSQAITRLSSGKRINSAADDAAGLAIATRMQTQINGL NQGVSNANDGVSILQTASSGLTSLTNSLQRIRQLAVQASNGPLSASDASALQQEVAQQIS EVNRIASQTNYNGKNILDGSAGTLSFQVGANVGQTVSVDLTQSMSAAKIGGGMVQTGQTL GTIKVAIDSSGAAWSSGS*(198)TGQETTQINVVSDGKGGFTFTDQNNQALSSTAVTAVFGSSTA GTGTAASPSFQTLALSTSATSALSATDQANATAMVAQINAVNKPQTVSNLDISTQTGAYQ AMVSIDNALATVNNLQATLGAAQNRFTAIATTQQAGSNNLAQAQSQIQSADFAQETANLS RAQVLQQAGISVLAQANSLPQQVLKLLQ |
| Sequence Around Glycosites (21 AA) | DSSGAAWSSGSTGQETTQINV |
| Technique(s) used for Glycosylation Detection | beta elimination of the Asp-N-digested peptide |
| Glycan Information | |
| Glycan Annotation | Glycan identified to be 291 Da in size and possessing acetamidino group and ammonia. |
| Technique(s) used for Glycan Identification | Trypsin deigestion and MS and MSMS |
| Protein Glycosylation linked (PGL) gene(s) | |
| Characterized Accessory Gene(s) | rmlB (BPSL2686) |
| Additional Comment | B. pseudomallei K96243 fliC gene was expressed in B. thailandensis E264 resulting in a glycosylated protein. The protein lacks S197, and therefore, at least, S198 must be the residue used for glycan attachment. |
| Literature | |
| Year of Identification | 2011 |
| Year of Identification Month Wise | 2011.07 |
| Reference | Scott, A.E., Twine, S.M., Fulton, K.M., Titball, R.W., Essex-Lopresti, A.E., Atkins, T.P. and Prior, J.L., 2011. Flagellar glycosylation in Burkholderia pseudomallei and Burkholderia thailandensis. Journal of bacteriology, 193(14), pp.3577-3587. |
| Corresponding Author | Andrew E Scott |
| Contact | Defence Science and Technology Laboratory, Porton Down, Salisbury SP4 0JQ, United Kingdom. |