ProGP527 (Probable tpr domain signal peptide protein)
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| ProGP ID | ProGP527 (Probable tpr domain signal peptide protein) |
| Validation Status | Characterized |
| Organism Information | |
| Organism Name | Ralstonia solanacearum GMI1000 |
| Domain | Bacteria |
| Classification | Family:Burkholderiaceae Order: Burkholderiales Class: Betaproteobacteria Division or phylum: "Proteobacteria" |
| Taxonomic ID (NCBI) | 267608 |
| Genome Information | |
| GenBank | NC_003295.1 |
| EMBL | AL646052.1 |
| Gene Information | |
| Gene Name | RSc1166 |
| NCBI Gene ID | 1219981 |
| GenBank Gene Sequence | NC_003295.1 |
| Protein Information | |
| Protein Name | Probable tpr domain signal peptide protein |
| UniProtKB/SwissProt ID | Q8Y078 |
| NCBI RefSeq | WP_011001116.1 |
| EMBL-CDS | CAD14868.1 |
| UniProtKB Sequence | >tr|Q8Y078|Q8Y078_RALSO Probable tpr domain signal peptide protein OS=Ralstonia solanacearum (strain GMI1000) GN=RSc1166 PE=4 SV=1 MNSTLRCRALGAALTGLIAMTTAPAFAQTAAPGIALPADLTPNTPQPPQVARQKRIDEWL ARKQYAQALAELDKDVATQPRNAQARFQRAVALAGLGRSDDAVVAFSQMTQDFPELPEPY LNLATLYAERGELLRARETLITATRVAPENAQAQANLGDIYVRLAAQSYQNALRLSPKHA AARARLDALPDLPGMRAVPSKPAAEPPAASGVNARPAKR |
| Sequence length | 219 AA |
| Glycosylation Status | |
| Glycosylation Type | O- (Ser/Thr) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | S210 |
| Glycosite(s) Annotated Protein Sequence | >tr|Q8Y078|Q8Y078_RALSO Probable tpr domain signal peptide protein OS=Ralstonia solanacearum (strain GMI1000) GN=RSc1166 PE=4 SV=1 MNSTLRCRALGAALTGLIAMTTAPAFAQTAAPGIALPADLTPNTPQPPQVARQKRIDEWL ARKQYAQALAELDKDVATQPRNAQARFQRAVALAGLGRSDDAVVAFSQMTQDFPELPEPY LNLATLYAERGELLRARETLITATRVAPENAQAQANLGDIYVRLAAQSYQNALRLSPKHA AARARLDALPDLPGMRAVPS*(210)KPAAEPPAASGVNARPAKR |
| Sequence Around Glycosites (21 AA) | SKPAAEPPAASGVNARPAKR |
| Technique(s) used for Glycosylation Detection | ZIC-HILIC |
| Technique(s) used for Glycosylated Residue(s) Detection | LC-MS, CID MS/MS and HCD MS/MS |
| Glycan Information | |
| Glycan Annotation | Pentasaccharide (HexNAc-(Pen)-dHex3) |
| Protein Glycosylation linked (PGL) gene(s) | |
| OST Gene Name | PglLRs |
| OST ProGT ID | ProGT104 (PglLRs) |
| Literature | |
| Reference | Elhenawy W, Scott NE, Tondo ML, Orellano EG, Foster LJ, Feldman MF. (2016) Protein O-linked glycosylation in the plant pathogen Ralstonia solanacearum. Glycobiology., 26(3):301-11. [PMID: 26531228] |
| Author | Elhenawy W, Scott NE, Tondo ML, Orellano EG, Foster LJ, Feldman MF. |
| Research Group | 1 Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada. 2 Centre for High-Throughput Biology, University of British Columbia, Vancouver, BC, Canada. 3 Faculty of Biochemical and Pharmaceutical Sciences (FBIOYF-UNR), Institute of Molecular and Cellular Biology of Rosario (IBR-CONICET), Rosario, Santa Fe, Argentina. 4 Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada Department of Molecular Microbiology, Washington University School of Medicine St. Louis, St. Louis, MO, USA |
| Corresponding Author | Feldman MF |
| Contact | Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada Department of Molecular Microbiology, Washington University School of Medicine St. Louis, St. Louis, MO, USA |