ProGP682 (Flagellin)
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| ProGP ID | ProGP682 (Flagellin) |
| Validation Status | Characterized |
| Organism Information | |
| Organism Name | Magnetospirillum magneticum AMB-1 |
| Domain | Bacteria |
| Classification | Family: Rhodospirillaceae Order: Rhodospirillales Class: Alphaproteobacteria Phylum:Proteobacteria |
| Taxonomic ID (NCBI) | 342108 |
| Genome Information | |
| GenBank | AP007255 |
| EMBL | AP007255 |
| Gene Information | |
| Gene Name | fla (amb0684) |
| GenBank Gene Sequence | BAE49488.1 |
| Protein Information | |
| Protein Name | Flagellin |
| UniProtKB/SwissProt ID | Q2W9I7 |
| NCBI RefSeq | WP_011383127.1 |
| EMBL-CDS | BAE49488.1 |
| UniProtKB Sequence | >tr|Q2W9I7|Q2W9I7_MAGSA Flagellin OS=Magnetospirillum magneticum (strain AMB-1 / ATCC 700264) OX=342108 GN=amb0684 PE=3 SV=1 MSDVTLTSAVRSNLLALQSTQGLVNRTQSRLSTGLKVGSAIDDPVAYFQSKALSDRASDF QGKKDNIDQGVSSLSTALQGISGVSTLVKQLKGLALNAQSASSSQIGSLVQQFNALRSQL DNLALDSQYQGQNLVAGKGQTLTVSFSNLTGSTLEVTSVDVRVGATGLNIAKAVTSNGGF QLSFDDASGAALGTEGVVKATYAGTATALTSGTYTFSYGSATVSFTVYSAGGTTGANADV EAFTTTSVLANGQDFNFRATTAGDTDELNVTQGSIQNNNHFAVEYHASTGGGVSGLAGGD SLNFVMNGLTGNSLASGNYTFSFGGYNMTFTVASAGDVTAGTFTTTNTFVNGTFGTAGGV TANTLALTLSTGTGTKENVVTFTAAVSSLGATSGPYKGQAYYSAGGISGAYGFQISAETK VGYVSGQYVLDSKLTGVVQNLVTQLDANLQVLRSHAQNLGTNVALLNTRLDFTKNYVDLL QSGSGKLTLADLNEEGANLLALQTRQQLGIQALSFAGQNEKSILSLFR |
| Sequence length | 528 AA |
| Subcellular Location | Flagellum |
| Function | Flagellin is the subunit of bacterial flagella |
| Glycosylation Status | |
| Glycosylation Type | O- (Ser/Thr) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | S103, S104, S145, S147 and T468 |
| Glycosite(s) Annotated Protein Sequence | >tr|Q2W9I7|Q2W9I7_MAGSA Flagellin OS=Magnetospirillum magneticum (strain AMB-1 / ATCC 700264) OX=342108 GN=amb0684 PE=3 SV=1 MSDVTLTSAVRSNLLALQSTQGLVNRTQSRLSTGLKVGSAIDDPVAYFQSKALSDRASDF QGKKDNIDQGVSSLSTALQGISGVSTLVKQLKGLALNAQSASS*(103)S*(104)QIGSLVQQFNALRSQL DNLALDSQYQGQNLVAGKGQTLTVS*(145)FS*(147)NLTGSTLEVTSVDVRVGATGLNIAKAVTSNGGF QLSFDDASGAALGTEGVVKATYAGTATALTSGTYTFSYGSATVSFTVYSAGGTTGANADV EAFTTTSVLANGQDFNFRATTAGDTDELNVTQGSIQNNNHFAVEYHASTGGGVSGLAGGD SLNFVMNGLTGNSLASGNYTFSFGGYNMTFTVASAGDVTAGTFTTTNTFVNGTFGTAGGV TANTLALTLSTGTGTKENVVTFTAAVSSLGATSGPYKGQAYYSAGGISGAYGFQISAETK VGYVSGQYVLDSKLTGVVQNLVTQLDANLQVLRSHAQNLGTNVALLNT*(468)RLDFTKNYVDLL QSGSGKLTLADLNEEGANLLALQTRQQLGIQALSFAGQNEKSILSLFR |
| Sequence Around Glycosites (21 AA) | GLALNAQSASS*SQIGSLVQQF LALNAQSASSS*QIGSLVQQFN VAGKGQTLTVS*FSNLTGSTLE GKGQTLTVSFS*NLTGSTLEVT NLGTNVALLNT*RLDFTKNYVD |
| Technique(s) used for Glycosylation Detection | Periodate/biotin hydrazine method |
| Technique(s) used for Glycosylated Residue(s) Detection | Tandem mass spectrometry (LC-MS, HCD-MS/MS)) |
| Protein Glycosylation- Implication | Flagellin glycosylation is required for export and assembly of flagellum. |
| Glycan Information | |
| Glycan Annotation | A nonulosonic acid sugar (most likely pseudaminic acid) |
| Literature | |
| Year of Identification | 2018 |
| Year of Validation | 2018 |
| Reference | Sulzenbacher G, Roig-Zamboni V, Lebrun R, Guérardel Y, Murat D, Mansuelle P, Yamakawa N, Qian XX, Vincentelli R, Bourne Y, Wu LF, Alberto F. (2018) Glycosylate and move! The glycosyltransferase Maf is involved in bacterial flagella formation.Environ Microbiol., 20(1):228-240. [PubMed: 29076618] |
| Author | Sulzenbacher G, Roig-Zamboni V, Lebrun R, Guérardel Y, Murat D, Mansuelle P, Yamakawa N, Qian XX, Vincentelli R, Bourne Y, Wu LF, Alberto F. |
| Research Group | 1 Aix Marseille Univ, CNRS, AFMB UMR7257, Marseille 13288, France. 2 Proteomic Platform, Institute of Microbiology of the Mediterranean, FR3479 Aix-Marseille University and National Center for Scientific Research, Marseille 13402, France. 3 Unit of Structural and Functional Glycobiology, UMR 8576 University of Lille and National Center for Scientific Research, Lille 59000, France. 4 Aix Marseille Univ, CNRS, LCB UMR7283, Marseille 13402, France. 5 International Associated Laboratory of Evolution and Development of Magnetotactic Organisms (LIA-MagMC), Centre National de la Recherche Scientifique, Marseille 13402, France. |
| Corresponding Author | Alberto F |
| Contact | International Associated Laboratory of Evolution and Development of Magnetotactic Organisms (LIA-MagMC), Centre National de la Recherche Scientifique, Marseille 13402, France. |