| Technique(s) used for Glycosylation Detection | Deglycosylation with anhydrous hydrogen fluoride. |
| Technique(s) used for Glycosylated Residue(s) Detection | Nano-LC-ES-MS/MS (nano-liquid chromatography-electrospray tandem mass spectrometry) |
| Protein Glycosylation- Implication | N-glycosylation endows H. volcanii with an ability to maintain an intact and stable cell envelope (correct S-layer architecture and stable association of the S-layer glycoprotein within the S-layer) in hypersaline surroundings, ensuring survival in this extreme environment. |
| Glycan Information |
| Glycan Annotation | Sulfoquinovose-hexose-based glycan, N-linked via a chitobiose core.
A trisaccharide corresponding to glucuronic acid (GlcA)-?1,4-GlcA-?1,4-glucose-?1-Asn, a tetrasaccharide corresponding to methyl-O-4-GlcA-?-1,4-galacturonic acid-?1,4-GlcA-?1,4-glucose-?1-Asn, and a pentasaccharide corresponding to hexose-1,2-[methyl-O-4-]GlcA-?-1,4-galacturonic acid-?1,4-GlcA-?1,4-glucose-?1-Asn.
The hexose at the non-reducing end of the pentasaccharide is a mannose.
In high salt (3.4 M NaCl) conditions, N13 and N83 are modified by a pentasaccharide while dolicholphosphate is modified by a tetrasaccharide comprising the first four pentasaccharide residues.
Cells grown at low salinity (1.75 M NaCl) possess dolichol phosphate and N498 residue (in S-layer glycoprotein) modified by a distinct tetrasaccharide comprising a sulfated hexose, two hexoses and a rhamnose.
A novel glycan, N-acetylglucosamine2-hexose2-(sulfoquinovose-hexose)6, is bound to Asn-732. |
| GlyTouCan | G31587VS |
| Technique(s) used for Glycan Identification | LC-ESI-MS, MALDI-TOF MS, Nano-LC-MS/MS and NMR |
| Protein Glycosylation linked (PGL) gene(s) |
| OST Gene Name | AglB |
| OST ProGT ID | ProGT15 (AglB) |
| Literature |
| Reference | Kandiba L, Lin CW, Aebi M, Eichler J, Guerardel Y. (2016) Structural characterization of the N-linked pentasaccharide decorating glycoproteins of the halophilic archaeon Haloferax volcanii. Glycobiology, 26(7), 745-756. [PubMed: 26863921] |
| Author | Kandiba L, Lin CW, Aebi M, Eichler J, Guerardel Y. |
| Research Group | 1
Department of Life Sciences, Ben Gurion University of the Negev, PO Box 653, Beersheva 84105, Israel.
2
Institute of Microbiology, ETH Zurich, CH-8093 Zurich, Switzerland.
3
Université de Lille, CNRS, UMR 8576-UGSF-Unité de Glycobiologie Structurale et Fonctionnelle, F 59000 Lille, France. |
| Corresponding Author | Eichler J, Guerardel Y. |
| Contact | Department of Life Sciences, Ben Gurion University of the Negev, PO Box 653, Beersheva 84105, Israel. |
| Reference | Parente J, Casabuono A, Ferrari MC, Paggi RA, De Castro RE, Couto AS, Giménez MI. (2014) A rhomboid protease gene deletion affects a novel oligosaccharide N-linked to the S-layer glycoprotein of Haloferax volcanii. J Biol Chem., 289(16), 11304-17. [PubMed: 24596091] |
| Author | Parente J, Casabuono A, Ferrari MC, Paggi RA, De Castro RE, Couto AS, Giménez MI. |
| Research Group | From the ‡Centro de Investigación en Hidratos de Carbono, Departamento de Química Orgánica, Facultad de Ciencias, Exactas y Naturales, Universidad de Buenos Aires, Pabellón II, Ciudad Universitaria, Buenos Aires, Argentina and
the §Instituto de Investigaciones Biológicas, Universidad Nacional de Mar del Plata-Consejo Nacional de Investigaciones Científicas y Técnicas, Funes 3250 4to nivel, 7600 Mar del Plata, Provincia de Buenos Aires, Argentina
3 To whom correspondence should be addressed. Tel.: 54-223-475-3030; Fax: 54-223-472-4143; E-mail: ra.ude.pdm@nemigim. |
| Corresponding Author | Giménez MI. |
| Contact | To whom correspondence should be addressed. Tel.: 54-223-475-3030; Fax: 54-223-472-4143; E-mail: ra.ude.pdm@nemigim. |
| Reference | Guan Z, Naparstek S, Calo D, Eichler J. (2012) Protein glycosylation as an adaptive response in Archaea: growth at different salt concentrations leads to alterations in Haloferax volcanii S-layer glycoprotein N-glycosylation. Environ Microbiol., 14(3), 743-53. [PubMed: 22029420] |
| Author | Guan Z, Naparstek S, Calo D, Eichler J. |
| Research Group | 1Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA
2Department of Life Sciences, Ben Gurion University, Beersheva 84105, Israel
*For correspondence. li.ca.ugb@relhciej; Tel. (+972) 8646 1343; Fax (+972) 8647 9175 |
| Corresponding Author | Eichler J. |
| Contact | Department of Life Sciences, Ben Gurion University, Beersheva 84105, Israel. Email: li.ca.ugb@relhciej; Tel. (+972) 8646 1343; Fax (+972) 8647 9175 |
| Reference | Kaminski, L., Abu-Qarn, M., Guan, Z., Naparstek, S., Ventura, V.V., Raetz, C.R., Hitchen, P.G., Dell, A. and Eichler, J. (2010) AglJ adds the first sugar of the N-linked pentasaccharide decorating the Haloferax volcanii S-layer glycoprotein. J Bacteriol, 192, 5572-5579. [PubMed: 20802039] |
| Author | Kaminski, L., Abu-Qarn, M., Guan, Z., Naparstek, S., Ventura, V.V., Raetz, C.R., Hitchen, P.G., Dell, A. and Eichler, J. |
| Research Group | Dept. of Life Sciences, Ben Gurion University, Beersheva 84105, Israel. |
| Corresponding Author | Eichler, J. |
| Contact | Dept. of Life Sciences, Ben Gurion University, Beersheva 84105, Israel. |
| Reference | Kaminski, L. and Eichler, J. (2010) Identification of residues important for the activity of Haloferax volcanii AglD, a component of the archaeal N-glycosylation pathway. Archaea, 2010, 315108. [PubMed: 20585355] |
| Author | Kaminski, L. and Eichler, J |
| Research Group | Department of Life Sciences, Ben Gurion University, Beersheva, Israel. |
| Corresponding Author | Eichler, J. |
| Contact | Department of Life Sciences, Ben Gurion University, Beersheva, Israel. |
| Reference | Magidovich, H., Yurist-Doutsch, S., Konrad, Z., Ventura, V.V., Dell, A., Hitchen, P.G. and Eichler, J. (2010) AglP is a S-adenosyl-L-methionine-dependent methyltransferase that participates in the N-glycosylation pathway of Haloferax volcanii. Mol Microbiol, 76, 190-199. [PubMed: 20149102] |
| Author | Magidovich, H., Yurist-Doutsch, S., Konrad, Z., Ventura, V.V., Dell, A., Hitchen, P.G. Eichler, J. |
| Research Group | Department of Life Sciences, Ben Gurion University, Beersheva, Israel. |
| Corresponding Author | Eichler, J. |
| Contact | Department of Life Sciences, Ben Gurion University, Beersheva, Israel. |
| Reference | Yurist-Doutsch, S., Magidovich, H., Ventura, V.V., Hitchen, P.G., Dell, A. and Eichler, J. (2010) N-glycosylation in Archaea: on the coordinated actions of Haloferax volcanii AglF and AglM. Mol Microbiol, 75, 1047-1058. [PubMed: 20487296] |
| Author | Yurist-Doutsch, S., Magidovich, H., Ventura, V.V., Hitchen, P.G., Dell, A. and Eichler, J |
| Research Group | Department of Life Sciences, Ben Gurion University of the Negev, Beersheva 84105, Israel. |
| Corresponding Author | Eichler, J. |
| Contact | Department of Life Sciences, Ben Gurion University of the Negev, Beersheva 84105, Israel. |
| Reference | Yurist-Doutsch, S. and Eichler, J. (2009) Manual annotation, transcriptional analysis, and protein expression studies reveal novel genes in the agl cluster responsible for N glycosylation in the halophilic archaeon Haloferax volcanii. J Bacteriol, 191, 3068-3075. [PubMed: 19251857] |
| Author | Yurist-Doutsch, S. Eichler, J. |
| Research Group | Dept. of Life Sciences, Ben Gurion University, P.O. Box 653, Beersheva 84105, Israel |
| Corresponding Author | Eichler, J. |
| Contact | Dept. of Life Sciences, Ben Gurion University, P.O. Box 653, Beersheva 84105, Israel |
| Reference | Abu-Qarn, M., Giordano, A., Battaglia, F., Trauner, A., Hitchen, P.G., Morris, H.R., Dell, A. and Eichler, J. (2008) Identification of AglE, a second glycosyltransferase involved in N glycosylation of the Haloferax volcanii S-layer glycoprotein. J Bacteriol, 190, 3140-3146. [PubMed: 18310347] |
| Author | Wieland, F., Heitzer, R. and Schaefer, W. |
| Research Group | Department of Life Sciences, Ben Gurion University, P.O. Box 653, Beersheva 84105, Israel. |
| Corresponding Author | Eichler, J |
| Contact | Department of Life Sciences, Ben Gurion University, P.O. Box 653, Beersheva 84105, Israel. |
| Reference | Yurist-Doutsch, S., Abu-Qarn, M., Battaglia, F., Morris, H.R., Hitchen, P.G., Dell, A. and Eichler, J. (2008) AglF, aglG and aglI, novel members of a gene island involved in the N-glycosylation of the Haloferax volcanii S-layer glycoprotein. Mol Microbiol, 69, 1234-1245. [PubMed: 18631242] |
| Author | Yurist-Doutsch, S., Abu-Qarn, M., Battaglia, F., Morris, H.R., Hitchen, P.G., Dell, A. and Eichler, J. |
| Research Group | Department of Life Sciences, Ben Gurion University, Beersheva 84105, Israel. |
| Corresponding Author | Eichler, J. |
| Contact | Department of Life Sciences, Ben Gurion University, Beersheva 84105, Israel. |
| Reference | Plavner, N. and Eichler, J. (2008) Defining the topology of the N-glycosylation pathway in the halophilic archaeon Haloferax volcanii. J Bacteriol, 190, 8045-8052. [PubMed: 18931126] |
| Author | Plavner, N. and Eichler, J. |
| Research Group | Department of Life Sciences, Ben Gurion University, Beersheva 84105, Israel. |
| Corresponding Author | Eichler, J. |
| Contact | Department of Life Sciences, Ben Gurion University, Beersheva 84105, Israel. |
| Reference | Abu-Qarn, M., Yurist-Doutsch, S., Giordano, A., Trauner, A., Morris, H.R., Hitchen, P., Medalia, O., Dell, A. and Eichler, J. (2007) Haloferax volcanii AglB and AglD are involved in N-glycosylation of the S-layer glycoprotein and proper assembly of the surface layer. J Mol Biol, 374, 1224-1236. [PubMed: 17996897] |
| Author | Abu-Qarn, M., Yurist-Doutsch, S., Giordano, A., Trauner, A., Morris, H.R., Hitchen, P., Medalia, O., Dell, A. and Eichler, J. |
| Research Group | Department of Life Sciences, Ben Gurion University, Beersheva 84105, Israel. |
| Corresponding Author | Eichler, J |
| Contact | Department of Life Sciences, Ben Gurion University, Beersheva 84105, Israel. |
| Reference | Abu-Qarn, M. and Eichler, J. (2006) Protein N-glycosylation in Archaea: defining Haloferax volcanii genes involved in S-layer glycoprotein glycosylation. Mol Microbiol, 61, 511-525. [PubMed: 16762024] |
| Author | Abu-Qarn, M. and Eichler, J. |
| Research Group | Department of Life Sciences, Ben Gurion University, Beersheva 84105, Israel. |
| Corresponding Author | Eichler, J. |
| Contact | Department of Life Sciences, Ben Gurion University, Beersheva 84105, Israel. |
| Reference | Eichler, J. (2000) Novel glycoproteins of the halophilic archaeon Haloferax volcanii. Arch Microbiol, 173, 445-448. [PubMed: 10896226] |
| Author | Eichler, J. |
| Research Group | Department of Life Sciences and The Doris and Bertie Black Center for Bioenergetics in Life Sciences, Ben Gurion University, Beersheva, Israel. |
| Corresponding Author | Eichler, J. |
| Contact | Department of Life Sciences and The Doris and Bertie Black Center for Bioenergetics in Life Sciences, Ben Gurion University, Beersheva, Israel. |
| Reference | Mengele, R. and Sumper, M. (1992) Drastic differences in glycosylation of related S-layer glycoproteins from moderate and extreme halophiles. J Biol Chem, 267, 8182-8185. [PubMed: 1569073] |
| Author | Mengele, R. and Sumper, M. |
| Research Group | Lehrstuhl Biochemie I, Universität Regensburg, Federal Republic of Germany. |
| Corresponding Author | Sumper, M. |
| Contact | Lehrstuhl Biochemie I, Universität Regensburg, Federal Republic of Germany. |
