| ProGT ID | ProGT15.4 (AglG) |
| Organism Information | |
| Organism Name | Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 /DS2) |
| Domain | Archaebacteria |
| Classification | Phylum : Euryarchaeota Class : Halobacteria Orders : Haloferacales Family : Haloreacaceae Genus : Haloferax Species : volcanii Strain : strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 /DS2 |
| Taxonomic ID (NCBI) | 309800 |
| Genome Information | |
| Gene Bank | CP001956.1 |
| EMBL | CP001956.1 |
| Gene Information | |
| Gene Name | aglG |
| NCBI Gene ID | 8926215 |
| NCBI Reference Sequence | NZ_AOHU01000097.1. |
| Protein information | |
| Protein Name | AglG |
| UniProtKB/ SwissProt ID | D4GYH3 |
| NCBI Ref Seq | WP_004041407.1. |
| UniProtKB Sequence | >sp|D4GYH3|AGLG_HALVD Glycosyltransferase AglG OS=Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) GN=aglG PE=1 SV=1 MKVSVVVCTYSMERYESFSETVESVLAQTYEPLELVIVVDGNEEEFDRVQDDFGDIDDVV LHCNDENRGISYSRTKGAELGTGDVVAMIDDDATAEPDWIETLVDTYENNPDAVAVGGTV VPDWVARKPEFFPEEFYWLVGCDERGFGEHMEEVRNTYGSNISFKRDVFLEVGGYDTNTG RKGDKHVQAHEAPVCIRIYEQTGERVIYNKQARVNHKLFEYRTEFDWLVFRSFWQGYSKR VMDLLYPQASDDKNAYLKDLMLVYVVDRLKNLVEDPSLAQVQQLIAIFVFTAAVGFGYVY GLLTPNLVEKTNN |
| EMBL CDS | CAQ51231.1. |
| Sequence length | 313 AA |
| Subcellular Location | Membrane (Integral component of membrane) |
| String | 309800.HVO_1529. |
| Glycosylation Information | |
| CAZY Family | GT2 |
| EC Number (BRENDA) | 2.4.1.- |
| Sugar Donor Specificity | UDP-Hexuronic acid |
| Acceptor Substrate Specificity | Dolichol phosphate-linked disaccharide. |
| Experimental Validation | In vivo |
| Product | Dolichol phosphate-hexose |
| Donor Specificity | UDP-Hexuronic acid |
| Function in Glycosylation pathway | 1) It is a glycosyltransferase. This compound was used to generate glucose-charged DolP, a precursor of the N-linked pentasaccharide. |
| Additional Information | 1) Deletion of aglG gene show, its involvement in the addition of a hexuronic acid found at position two. |
| Litrature | |
| Year Of Validation | 2008 |
| Reference | Yurist?Doutsch, S., Abu?Qarn, M., Battaglia, F., Morris, H.R., Hitchen, P.G., Dell, A. and Eichler, J., 2008. aglF, aglG and aglI, novel members of a gene island involved in the N?glycosylation of the Haloferax volcanii S?layer glycoprotein. Molecular microbiology, 69(5), pp.1234-1245. |
| Corresponding Author | Department of Life Sciences, Ben Gurion University of the Negev, Beersheva, Israel. |
| Reference | Elharar, Y., Podilapu, A.R., Guan, Z., Kulkarni, S.S. and Eichler, J., 2017. Assembling glycan-charged dolichol phosphates: chemoenzymatic synthesis of a Haloferax volcanii N-glycosylation pathway intermediate. Bioconjugate chemistry, 28(9), pp.2461-2470. |
| Corresponding Author | Department of Chemistry, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India Department of Life Sciences, Ben Gurion University of the Negev, Beersheva 8410501, Israel |