| ProGT ID | ProGTNC10 (WbpD) |
| Organism Information | |
| Organism Name | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRRS 101 / PAO1) |
| Domain | Bacteria |
| Classification | Phylum : Proteobacteria Class : Gammaproteobacteria Orders : Pseudomonadales Family : Pseudomanadaceae Genus : Pseudomonas Species : aeruginosa Strain : ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRRS 101 / PAO1 |
| Taxonomic ID (NCBI) | 208964 |
| Genome Information | |
| Gene Bank | AE004091.2 |
| EMBL | AE004091.2 |
| Gene Information | |
| Gene Name | wbpD |
| NCBI Gene ID | 882593 |
| Protein information | |
| Protein Name | WbpD |
| UniProtKB/ SwissProt ID | G3XD01 |
| NCBI Ref Seq | WP_003113426.1 |
| UniProtKB Sequence | >sp|G3XD01|WBPD_PSEAE UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronate N-acetyltransferase OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=wbpD PE=1 SV=1 MSYYQHPSAIVDDGAQIGSDSRVWHFVHICAGARIGAGVSLGQNVFVGNKVVIGDRCKIQ NNVSVYDNVTLEEGVFCGPSMVFTNVYNPRSLIERKDQYRNTLVKKGATLGANCTIVCGV TIGEYAFVGAGAVINKNVPSYALMVGVPARQIGWMSEFGEQLQLNEQGEAVCSHSGARYV LNGKILSKVDV |
| EMBL CDS | AAG06544 |
| Sequence length | 191 AA |
| String | 208964.PA3156 |
| Glycosylation Information | |
| EC Number (BRENDA) | 2.3.1.201 |
| Acceptor Substrate Specificity | UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate |
| Product | UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate |
| Function in Glycosylation pathway | 1) Responsible for the transfer of an acetyl group to the C-3 amino position of UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate (UDP-D-GlcNAc3NA). |
| Litrature | |
| Year Of Validation | 2008 |
| Reference | Westman, E.L., Preston, A., Field, R.A. and Lam, J.S., 2008. Biosynthesis of a rare di-N-acetylated sugar in the lipopolysaccharides of both Pseudomonas aeruginosa and Bordetella pertussis occurs via an identical scheme despite different gene clusters. Journal of bacteriology, 190(18), pp.6060-6069. |
| Corresponding Author | 1 University of Guelph, Department of Molecular and Cellular Biology, Guelph, Ontario, Canada. |
| Reference | Larkin, A. and Imperiali, B., 2009. Biosynthesis of UDP-GlcNAc (3NAc) A by WbpB, WbpE, and WbpD: enzymes in the Wbp pathway responsible for O-antigen assembly in Pseudomonas aeruginosa PAO1. Biochemistry, 48(23), pp.5446-5455. |
| Corresponding Author | 1 Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge,Massachusetts 02139, USA. |
| Reference | Westman, E.L., McNally, D.J., Charchoglyan, A., Brewer, D., Field, R.A. and Lam, J.S., 2009. Characterization of WbpB, WbpE, and WbpD and reconstitution of a pathway for the biosynthesis of UDP-2, 3-diacetamido-2, 3-dideoxy-D-mannuronic acid in Pseudomonas aeruginosa. Journal of Biological Chemistry, 284(18), pp.11854-11862. |
| Corresponding Author | 1 Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada. |
| Reference | Thoden, J.B. and Holden, H.M., 2010. Structural and functional studies of WlbA: A dehydrogenase involved in the biosynthesis of 2, 3-diacetamido-2, 3-dideoxy-D-mannuronic acid. Biochemistry, 49(36), pp.7939-7948. |
| Corresponding Author | 1 Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA. |