ProGT106 (SseK3)
| ProGT ID | ProGT106 (SseK3) |
| Organism Information | |
| Organism Name | Salmonella typhimurium (strain SL1344) |
| Clinical Implication | Pathogenic |
| Domain | Bacteria |
| Classification | Phylum : Proteobacteria Class : GammaProteobacteria Orders : Enterobacterales Family : Enteerobacteriaceae Genus : Salmonella Species : typhimurium Strain : SL1344 |
| Taxonomic ID (NCBI) | 216597 |
| Genome Information | |
| Gene Bank | FQ312003 |
| EMBL | FQ312003 |
| Gene Information | |
| Gene Name | sseK3 |
| Protein information | |
| Protein Name | SseK3 |
| UniProtKB/ SwissProt ID | A0A0H3NMP8 |
| NCBI Ref Seq | WP_000492926.1? |
| UniProtKB Sequence | >tr|A0A0H3NMP8|A0A0H3NMP8_SALTS Type III secretion system effector protein OS=Salmonella typhimurium (strain SL1344) GN=sseK3 PE=4 SV=1 MFSRVRGFLSCQNYSHTATPAITLPSSGSANFAGVEYPLLPLDQHTPLLFQWFERNPSRF GENQIPIINTQQNPYLNNIINAAIIEKERTIGVLVDGNFSAGQKKALAKLEKQYENIKVI YNSDLDYSMYDKKLSDIYLENIAKIEAQPANVRDEYLLGEIKKSLNEVLKNNPEESLVSS HDKRLGHVRFDFYRNLFLLKGSNAFLEAGKHGCHHLQPGGGCIYLDADMLLTGKLGTLYL PDGIAVHVSRKGNSMSLENGIIAVNRSEHPALKKGLEIMHSKPYGDPYIDGVCGGLRHYF NCSIRHNYEEFCNFIEFKHEHIFMDTSSLTISSWR |
| EMBL CDS | CBW18025.1 |
| Sequence length | 335 AA |
| Function in Native Organism | 1) The SseK3 translocates into host cells, targeting innate immune responses, including NF-?B activation. |
| Additional Information | 1) SseK3 is the first structure of a retaining arginine glycosyltransferase bound to a hydrolyzed form of its donor substrate. 2) SseK3-mediated inhibition of TNF-alpha-induced NF-?B activation, directly correlating enzymatic activity to virulence function. |
| Glycosyltransferase Information | |
| Glycosylation Type | N- (Arg) linked |
| EC Number (BRENDA) | 2.4.99.18 |
| Mechanism of Glycan Transfer | Sequential |
| Donor Type | UDP-GlcNAc |
| Donor Specificity | Nucleotide activated sugars |
| Glycan Information | |
| Glycan transferred | Monosaccharide (GlcNAc) |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | TRADD |
| Litrature | |
| Year Of Validation | 2015 |
| Reference | Esposito, D., Günster, R.A., Martino, L., El Omari, K., Wagner, A., Thurston, T.L. and Rittinger, K., 2018. Structural basis for the glycosyltransferase activity of the Salmonella effector SseK3. Journal of Biological Chemistry, 293(14), pp.5064-5078. |
| Corresponding Author | 1 Molecular Structure of Cell Signalling Laboratory, Francis Crick Institute, 1 Midland Road, London NW1 1AT, United Kingdom.
2 Section of Microbiology, Medical Research Council Centre for Molecular Bacteriology and Infection, Imperial College London, London SW7 2AZ, United Kingdom.
3 Diamond Light Source, Harwell Science and Innovation Campus, Chilton, Didcot OX11 0DE, United Kingdom.
4 Section of Microbiology, Medical Research Council Centre for Molecular Bacteriology and Infection, Imperial College London, London SW7 2AZ, United Kingdom.
5 Molecular Structure of Cell Signalling Laboratory, Francis Crick Institute, 1 Midland Road, London NW1 1AT, United Kingdom. |