ProGT113 (Gtf1)
| ProGT ID | ProGT113 (Gtf1) |
| Organism Information | |
| Organism Name | Streptococcus gordonii |
| Clinical Implication | Pathogenic |
| Domain | Bacteria |
| Classification | Phylum : Firmicutes Class : Bacilli Orders : Lactobacillales Family : Streptococcaceae Genus : Streptococcus Species : gordonii |
| Taxonomic ID (NCBI) | 1302 |
| Genome Information | |
| Gene Bank | AY028381 |
| EMBL | AY028381 |
| Gene Information | |
| Gene Name | gtf1 |
| Protein information | |
| Protein Name | Gtf1 |
| UniProtKB/ SwissProt ID | Q9AET5 |
| UniProtKB Sequence | >sp|Q9AET5|GTF1_STRGN Glycosyltransferase Gtf1 OS=Streptococcus gordonii GN=gtf1 PE=1 SV=2 MTVYNINLGIGWASSGVEYAQAYRAQILRRIQQPAKFIFMDMILADNIQHLTENIGFLDE EIIWLYNYFTDIKIAPTTVTLDQVLAQVAGQPERSEKEGKIVRYFYPQDDQFITCYLRQE DQDSVEHVEYVSRGRLIRKDYFSYVRYASEYFAPHNDAATLYQRRFYHEDGSVAYDMLIE DGQEKLYRFPDRIFYSKAELVRYFLQCLQLQADDVVILDRETGIGQVVFEESQKAKLGVV VHAEHFSENASSDDYILWNNFYDYQFTNADKVDFFIVATEAQKRILEQQFQHYSDKQPQI ATIPVGSLDQLTYPKEPRKPYSMITASRLATEKHIDWLVAATVQAHAQLPELTLDIYGKG SEEDKLRRRIEEAGAQDYIRLKGHADLSQIYAGYELYLTASTSEGFGLTLMEAVGSGLPL IGFDVRYGNQTFIDDGKNGYLLPVSSNHVEDQIIAAFVEKIIALFSQGRQQEMSQHSYQV AENYLTSRVEAAWTQLLKEVRDDSAL |
| EMBL CDS | AAK17002 |
| Sequence length | 506 AA |
| Subcellular Location | Membrane (Integral component of membrane) |
| Function in Native Organism | 1) Deletion of GtfA abolishes adhesin secretion in Streptococcus gordonii. |
| Additional Information | 1) GtfA made a complex with GtfB and together both required for modification of Ser/Thr-rich repeats of adhesin in S. gordonii. 2) Only GtfA contains an active site for glycosylation. |
| Glycosyltransferase Information | |
| Glycosylation Type | O- (Ser/Thr) linked |
| CAZY Family | GT4 |
| EC Number (BRENDA) | 2.4.99.18 |
| Mechanism of Glycan Transfer | Sequential |
| Donor Type | UDP-GlcNAc |
| Donor Specificity | Nucleotide activated sugars |
| Glycan Information | |
| Glycan transferred | Monosaccharide (GlcNAc) |
| Experimental_strategies | In vivo and In vitro |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | GspB |
| ProGPdb ID | ProGP229 |
| Litrature | |
| Year Of Validation | 2016 |
| Reference | Chen, Y., Seepersaud, R., Bensing, B.A., Sullam, P.M. and Rapoport, T.A., 2016. Mechanism of a cytosolic O-glycosyltransferase essential for the synthesis of a bacterial adhesion protein. Proceedings of the National Academy of Sciences, 113(9), pp.E1190-E1199. |
| Corresponding Author | 1 Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115. 2 Department of Cell Biology, Harvard Medical School, Boston. |