ProGT140 (Maf)
| ProGT ID | ProGT140 (Maf) |
| Organism Information | |
| Organism Name | Geobacillus kaustophilus HTA426 |
| Domain | Bacteria |
| Classification | Phylum : Firmicutes Class : Bacilli Orders : Bacillales Family : Bacillaceae Genus : Geobacillus Species : kaustophilus Strain : HTA426 |
| Taxonomic ID (NCBI) | 235909 |
| Genome Information | |
| Gene Information | |
| Gene Name | maf |
| Protein information | |
| Protein Name | Maf |
| NCBI Ref Seq | BAD77414.1 |
| Function in Native Organism | It is sialyltransferase and transfer Neu5Ac to flagella |
| Additional Information | D285A and N379A in Maf are important for its GT activities |
| Glycosyltransferase Information | |
| Glycosylation Type | O- (Ser/Thr) linked |
| Mechanism of Glycan Transfer | Sequential |
| Donor Type | CMP-Neu5Ac |
| Donor Specificity | Nucleotide activated sugars |
| Glycan Information | |
| Glycan transferred | Monosaccharide (Neu5Ac) |
| Method of Glycan Indentification | LC–MS/MS and Western blotting |
| Experimental_strategies | In vivo |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | GkFlaA1 |
| ProGPdb ID | ProGP1211 (GkFlaA1) |
| Acceptor Substrate name | GkFlaA2 |
| ProGPdb ID | ProGP1212 (GkFlaA2) |
| Litrature | |
| Year Of Validation | 2021 |
| Reference | Khairnar, A., Sunsunwal, S., Babu, P. and Ramya, T.N.C., 2021. Novel serine/threonine-O-glycosylation with N-acetylneuraminic acid and 3-deoxy-D-manno-octulosonic acid by bacterial flagellin glycosyltransferases. Glycobiology, 31(3), pp.288-306. |
| Corresponding Author | Department of Protein Science and Engineering, CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh 160036, India |