ProGT23 (AglB)
| ProGT ID | ProGT23 (AglB) |
| Organism Information | |
| Organism Name | Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) |
| Clinical Implication | Non-pathogenic |
| Domain | Archaebacteria |
| Phylum | Euryarchaeota |
| Classification | Family: Thermococcaceae Order: Thermococcales Class: Thermococci or Protoarchaea Division or phylum: "Euryarchaeota" |
| Taxonomic ID (NCBI) | 186497 |
| Genome Information | |
| Gene Bank | AE009950 |
| EMBL | AE009950 |
| Gene Information | |
| Gene Name | PF0156 |
| NCBI Gene ID | 1467988 |
| Protein information | |
| Protein Name | AglBÂ |
| UniProtKB/ SwissProt ID | Q8U4D2 |
| NCBI Ref Seq | NC_003413.1. |
| UniProtKB Sequence | >tr|Q8U4D2|Q8U4D2_PYRFU Oligosaccharyl transferase stt3 subunit related protein OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) GN=PF0156 PE=1 SV=1 MVKTQIKEKKKDEKVTIPLPGKIKTVLAFLVVLAFAAYGFYIRHLTAGKYFSDPDTFYHF EIYKLVLKEGLPRYYPMADAPFGSLIGEPLGLYILPAIFYKIISIFGYNELEAFLLWPPF VGFLSVIGVYLLGRKVLNEWAGMWGAIILSVLTANFSRTFSGNARGDGPFMMLFTFSAVL MLYYLTEENKNKKIIWGTLFVLLAGISTAAWNGSPFGLMVLLGFASFQTIILFIFGKINE LREFIKEYYPAYLGILAISYLLTIPGIGKIGGFVRFAFEVFLGLVFLAIVMLYGGKYLNY SDKKHRFAVVAVIVIAGFAGAYIYVGPKLFTLMGGAYQSTQVYETVQELAKTDWGDVKVY YGVEKPNGIVFFLGLVGAMIVTARYLYKLFKDGRRPHEELFAITFYVMSIYLLWTAARFL FLASYAIALMSGVFAGYVLETVEKMKESIPIKAALGGVIAIMLLLIPLTHGPLLAQSAKS MRTTEIETSGWEDALKWLRENTPEYSTATSWWDYGYWIESSLLGQRRASADGGHARDRDH ILALFLARDGNISEVDFESWELNYFLVYLNDWAKFNAISYLGGAITRREYNGDESGRGAV TTLLPLPRYGEKYVNLYAKVIVDVSNSSVKVTVGDRECDPLMVTFTPSGKTIKGTGTCSD GNAFPYVLHLTPTIGVLAYYKVATANFIKLAFGVPASTIPGFSDKLFSNFEPVYESGNVI VYRFTPFGIYKIEENINGTWKQVYNLTPGKHELKLYISAFGRDIENATLYIYAINNEKII EKIKIAEISHMDYLNEYPIAVNVTLPNATSYRFVLVQKGPIGVLLDAPKVNGEIRSPTNI LREGESGEIELKVGVDKDYTADLYLRATFIYLVRKSGKDNEDYDAAFEPQMDVFFITKIG ENIQLKEGENTVKVRAELPEGVISSYKDELQRKYGDKLIIRGIRVEPVFIAEKEYLMLEV SASAPHH |
| EMBL CDS | AAL80280.1 |
| Sequence length | 967 AA |
| Subcellular Location | Membrane (Integral component of membrane) |
| String | 186497.PF0156. |
| Potential Application | 1) Design of OST mutational variants could be use for the production of homogeneous glycoproteins in engineered mammalian and Escherichia coli cells. |
| Additional Information | 1) Pyrococcus furiosus OST contains a newly identified conserved motif DxxK (DK motif), which is located very close to the conserved WWDYG motif in the tertiary structure. |
| PDB ID | 2ZAG 2ZAI |
| Glycosyltransferase Information | |
| Glycosylation Type | N- (Asn) linked |
| CAZY Family | GT66 |
| EC Number (BRENDA) | 2.4.99.18.5243. |
| Mechanism of Glycan Transfer | En bloc |
| Donor Type | Lipid linked sugars |
| Donor Specificity | DolPP-Heptasaccharide |
| Glycan Information | |
| Glycan transferred | Heptasaccharide (one hexouronate and two pentose residues)Â |
| Method of Glycan Indentification | MALDI-QIT-TOF MS |
| Experimental_strategies | In vitro |
| Acceptor Subtrate Information | |
| Acceptor Substrate name | Polypeptide containing N-X-S/T consensus sequence |
| Litrature | |
| Year Of Validation | 2007Â |
| Reference | Igura, M., Maita, N., Obita, T., Kamishikiryo, J., Maenaka, K., & Kohda, D. (2007). Purification, crystallization and preliminary X?ray diffraction studies of the soluble domain of the oligosaccharyltransferase STT3 subunit from the thermophilic archaeon Pyrococcus furiosus. Acta Crystallographica Section F, 63(9), 798-801. |
| Authors | Igura, M., Maita, N., Obita, T., Kamishikiryo, J., Maenaka, K., & Kohda, D. |
| Research groups | Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan. |
| Corresponding Author | Kohda, D. |
| Contacts | Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan. |
| Reference | Igura, M., Maita, N., Kamishikiryo, J., Yamada, M., Obita, T., Maenaka, K., & Kohda, D. (2008). Structure?guided identification of a new catalytic motif of oligosaccharyltransferase. The EMBO journal, 27(1), 234-243. |
| Authors | Igura, M., Maita, N., Kamishikiryo, J., Yamada, M., Obita, T., Maenaka, K., & Kohda, D. |
| Research groups | Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan. |
| Corresponding Author | Kohda, D. |
| Contacts | Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan. |
| Reference | Igura, M., & Kohda, D. (2011). Selective control of oligosaccharide transfer efficiency for the N-glycosylation sequon by a point mutation in oligosaccharyltransferase. Journal of Biological Chemistry, jbc-M110. |
| Authors | Igura, M., & Kohda, D. |
| Research groups | Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, Maidashi 3-1-1, Higashi-ku,Fukuoka 812-8582, Japan |
| Corresponding Author | Kohda, D. |
| Contacts | Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, Maidashi 3-1-1, Higashi-ku,Fukuoka 812-8582, Japan |