| ProGT ID | ProGT5 (TcsL) |
| Organism Information |
| Organism Name | Clostridium sordellii 6018 |
| Clinical Implication | Pathogenic |
| Domain | Bacteria |
| Phylum | Firmicutes |
| Classification | Family: Clostridiaceae
Order: Clostridiales
Class: Clostridia
Phylum: Firmicutes |
| Taxonomic ID (NCBI) | 1505 |
| Genome Information |
| Gene Information |
| Gene Name | tcsL |
| NCBI Reference Sequence | X82638 |
| Protein information |
| Protein Name | TcsLÂ |
| UniProtKB/ SwissProt ID | Q46342 |
| UniProtKB Sequence | >tr|Q46342|Q46342_PAESO Cytotoxin L OS=Paeniclostridium sordellii PE=1 SV=1
MNLVNKAQLQKMVYVKFRIQEDEYVAILNALEEYHNMSESSVVEKYLKLKDINNLTDNYL
NTYKKSGRNKALKKFKEYLTMEVLELKNNSLTPVEKNLHFIWIGGQINDTAINYINQWKD
VNSDYTVKVFYDSNAFLINTLKKTIVESATNNTLESFRENLNDPEFDYNKFYRKRMEIIY
DKQKHFIDYYKSQIEENPEFIIDNIIKTYLSNEYSKDLEALNKYIEESLNKITANNGNDI
RNLEKFADEDLVRLYNQELVERWNLAAASDILRISMLKEDGGVYLDVDILPGIQPDLFKS
INKPDSITNTSWEMIKLEAIMKYKEYIPGYTSKNFDMLDEEVQRSFESALSSKSDKSEIF
LPLDDIKVSPLEVKIAFANNSVINQALISLKDSYCSDLVINQIKNRYKILNDNLNPSINE
GTDFNTTMKIFSDKLASISNEDNMMFMIKITNYLKVGFAPDVRSTINLSGPGVYTGAYQD
LLMFKDNSTNIHLLEPELRNFEFPKTKISQLTEQEITSLWSFNQARAKSQFEEYKKGYFE
GALGEDDNLDFAQNTVLDKDYVSKKILSSMKTRNKEYIHYIVQLQGDKISYEASCNLFSK
DPYSSILYQKNIEGSETAYYYYVADAEIKEIDKYRIPYQISNKRNIKLTFIGHGKSEFNT
DTFANLDVDSLSSEIETILNLAKADISPKYIEINLLGCNMFSYSISAEETYPGKLLLKIK
DRVSELMPSISQDSITVSANQYEVRINEEGKREILDHSGKWINKEESIIKDISSKEYISF
NPKENKIIVKSKYLHELSTLLQEIRNNANSSDIDLEKKVMLTECEINVASNIDRQIVEGR
IEEAKNLTSDSINYIKNEFKLIESISDSLYDLKHQNGLDDSHFISFEDISKTENGFRIRF
INKETGNSIFIETEKEIFSEYATHISKEISNIKDTIFDNVNGKLVKKVNLDAAHEVNTLN
SAFFIQSLIEYNTTKESLSNLSVAMKVQVYAQLFSTGLNTITDASKVVELVSTALDETID
LLPTLSEGLPIIATIIDGVSLGAAIKELSETNDPLLRQEIEAKIGIMAVNLTAASTAIVT
SALGIASGFSILLVPLAGISAGIPSLVNNELILQDKATKVIDYFKHISLAETEGAFTLLD
DKIIMPQDDLVLSEIDFNNNSITLGKCEIWRAEGGSGHTLTDDIDHFFSSPSITYRKPWL
SIYDVLNIKKEKIDFSKDLMVLPNAPNRVFGYEMGWTPGFRSLDNDGTKLLDRIRDHYEG
QFYWRYFAFIADALITKLKPRYEDTNVRINLDGNTRSFIVPVITTEQIRKNLSYSFYGSG
GSYSLSLSPYNMNIDLNLVENDTWVIDVDNVVKNITIESDEIQKGELIENILSKLNIEDN
KIILNNHTINFYGDINESNRFISLTFSILEDINIIIEIDLVSKSYKILLSGNCMKLIENS
SDIQQKIDHIGFNGEHQKYIPYSYIDNETKYNGFIDYSKKEGLFTAEFSNESIIRNIYMP
DSNNLFIYSSKDLKDIRIINKGDVKLLIGNYFKDDMKVSLSFTIEDTNTIKLNGVYLDEN
GVAQILKFMNNAKSALNTSNSLMNFLESINIKNIFYNNLDPNIEFILDTNFIISGSNSIG
QFELICDKDKNIQPYFINFKIKETSYTLYVGNRQNLIVEPSYHLDDSGNISSTVINFSQK
YLYGIDRYVNKVIIAPNLYTDEINITPVYKPNYICPEVIILDANYINEKINVNINDLSIR
YVWDNDGSDLILIANSEEDNQPQVKIRFVNVFKSDTAADKLSFNFSDKQDVSVSKIISTF
SLAAYSDGFFDYEFGLVSLDNDYFYINSFGNMVSGLIYINDSLYYFKPPKNNLITGFTTI
DGNKYYFDPTKSGAASIGEITIDGKDYYFNKQGILQVGVINTSDGLKYFAPAGTLDENLE
GESVNFIGKLNIDGKIYYFEDNYRAAVEWKLLDDETYYFNPKTGEALKGLHQIGDNKYYF
DDNGIMQTGFITINDKVFYFNNDGVMQVGYIEVNGKYFYFGKNGERQLGVFNTPDGFKFF
GPKDDDLGTEEGELTLYNGILNFNGKIYFFDISNTAVVGWGTLDDGSTYYFDDNRAEACI
GLTVINDCKYYFDDNGIRQLGFITINDNIFYFSESGKIELGYQNINGNYFYIDESGLVLI
GVFDTPDGYKYFAPLNTVNDNIYGQAVKYSGLVRVNEDVYYFGETYKIETGWIENETDKY
YFDPETKKAYKGINVVDDIKYYFDENGIMRTGLISFENNNYYFNEDGKMQFGYLNIKDKM
FYFGKDGKMQIGVFNTPDGFKYFAHQNTLDENFEGESINYTGWLDLDGKRYYFTDEYIAA
TGSLTIDGYNYYFDPDTAELVVSE
|
| EMBL CDS | CAA57959.1. |
| Sequence length | 2364 AA |
| Subcellular Location | Host cell plasma membrane |
| Function in Native Organism | 1) It is a secretory glycosyltransferase which glycosylates the Rho family proteins of the host cell. |
| Potential Application | 1) Castanospermine binds to Rho/Ras-glucosylating Clostridium sordellii lethal toxin and Clostridium di?cile toxin B and inhibits the effect of toxins therefore this compound has a potential lead structure for the development of therapeutic inhibitors of clostridial glucosylating toxins. |
| Additional Information | 1) Site-directed mutagenesis Ile-383 and Gln-385 in C. sordellii lethal toxin to serine and alanine, resulting in the change of the donor substrate specificity from UDP-glucose to UDP-GlcNAc. |
| PDB ID | 2VKD[A B C] 2VKH 2VL8 |
| Glycosyltransferase Information |
| Glycosylation Type | O- (Thr) linked |
| CAZY Family | GT44 |
| EC Number (BRENDA) | 2.4.1.B62.1514. |
| Mechanism of Glycan Transfer | Sequential |
| Donor Type | Nucleotide activated sugars |
| Donor Specificity | UDP-Glc |
| Glycan Information |
| Glycan transferred | Monosaccharide (Glc)Â |
| Experimental_strategies | In vitro |
| Acceptor Subtrate Information |
| Acceptor Substrate name | RhoA |
| Acceptor Substrate name | Rac1 |
| Acceptor Substrate name | Ras |
| Acceptor Substrate name | Cdc42 |
| Litrature |
| Year Of Validation | 1995Â |
| Reference | Green, G. A., Schué, V., & Monteil, H. (1995). Cloning and characterization of the cytotoxin L-encoding gene of Clostridium sordellii: homology with Clostridium difficile cytotoxin B. Gene, 161(1), 57-61.
|
| Authors | Green, G. A., Schué, V., & Monteil, H.
|
| Research groups | Laboratory of Bacterial Toxinology, University Louis-Pasteur, Strasbourg, France.
|
| Corresponding Author | Monteil, H.
|
| Contacts | Laboratory of Bacterial Toxinology, University Louis-Pasteur, Strasbourg, France.
|
| Reference | Green, G. A., Schue, V., Girardot, R., & Monteil, H. (1996). Characterisation of an enterotoxin-negative, cytotoxin-positive strain of Clostridium sordellii. Journal of medical microbiology, 44(1), 60-64.
|
| Authors | Green, G. A., Schue, V., Girardot, R., & Monteil, H.
|
| Research groups | Laboratory of Bacterial Toxinology, University Louis-Pasteur, Strasbourg, France.
|
| Corresponding Author | Monteil, H.
|
| Contacts |
Laboratory of Bacterial Toxinology, University Louis-Pasteur, Strasbourg, France.
|
| Reference | Jank, T., Ziegler, M. O., Schulz, G. E., & Aktories, K. (2008). Inhibition of the glucosyltransferase activity of clostridial Rho/Ras?glucosylating toxins by castanospermine. FEBS letters, 582(15), 2277-2282.
|
| Authors | Jank, T., Ziegler, M. O., Schulz, G. E., & Aktories, K.
|
| Research groups | Institute of Experimental and Clinical Pharmacology and Toxicology, Albert-Ludwigs-University, Albertstrasse 25, Freiburg, Germany
|
| Corresponding Author | Aktories, K.
|
| Contacts | Institute of Experimental and Clinical Pharmacology and Toxicology, Albert-Ludwigs-University, Albertstrasse 25, Freiburg, Germany
|
| Reference | Ziegler, M. O., Jank, T., Aktories, K., & Schulz, G. E. (2008). Conformational changes and reaction of clostridial glycosylating toxins. Journal of molecular biology, 377(5), 1346-1356.
|
| Authors | Ziegler, M. O., Jank, T., Aktories, K., & Schulz, G. E.
|
| Research groups | Institute of Organic Chemistry and Biochemistry, Albert-Ludwigs-University, Albertstr. 21, D-79104 Freiburg im Breisgau, Germany
|
| Corresponding Author | Schulz, G. E.
|
| Contacts | Institute of Organic Chemistry and Biochemistry, Albert-Ludwigs-University, Albertstr. 21, D-79104 Freiburg im Breisgau, Germany
|
| Reference | Huelsenbeck, S. C., Klose, I., Reichenbach, M., Huelsenbeck, J., & Genth, H. (2009). Distinct kinetics of (H/K/N) Ras glucosylation and Rac1 glucosylation catalysed by Clostridium sordellii lethal toxin. FEBS letters, 583(19), 3133-3139.
|
| Authors | Huelsenbeck, S. C., Klose, I., Reichenbach, M., Huelsenbeck, J., & Genth, H.
|
| Research groups | Institute of Toxicology, Hannover Medical School, D-30625 Hannover, Germany
|
| Corresponding Author | Genth, H.
|
| Contacts | Institute of Toxicology, Hannover Medical School, D-30625 Hannover, Germany
|
| Reference | Varela Chavez, C., Haustant, G.M., Baron, B., England, P., Chenal, A., Pauillac, S., Blondel, A & Popoff, M. R. (2016). The tip of the four N-Terminal ?-helices of Clostridium sordellii lethal toxin contains the interaction site with membrane phosphatidylserine facilitating small GTPases glucosylation. Toxins, 8(4), 90.
|
| Authors | Varela Chavez, C., Haustant, G.M., Baron, B., England, P., Chenal, A., Pauillac, S., Blondel, A & Popoff, M. R.
|
| Research groups | Institute of Toxicology, Hannover Medical School, D-30625 Hannover, Germany
|
| Corresponding Author | Popoff, M. R.
|
| Contacts | Institute of Toxicology, Hannover Medical School, D-30625 Hannover, Germany
|
