ProGT76 (GtfB)

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ProGT ID ProGT76 (GtfB)
ProGT Pathway
Organism Information
Organism NameStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Clinical ImplicationPathogenic
DomainBacteria
Classification Phylum : Firmicutes
Class : Bacilli
Orders : Lactobacillales
Family : Streptococcaceae
Genus : Streptococcus
Species : pneumoniae
Strain : ATCC BAA-334 / TIGR4
Taxonomic ID (NCBI)170187
Genome Information
Gene BankAE005672
EMBLAE005672
Gene Information
Gene NamegtfB
NCBI Reference SequenceNZ_AKVY01000001.1. 
Protein information
Protein NameGtfB 
UniProtKB/ SwissProt IDA0A0H2UR90
NCBI Ref SeqWP_000571755.1
UniProtKB Sequence>tr|A0A0H2UR90|A0A0H2UR90_STRPN Glycosyltransferase stabilizing protein Gtf2 OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) GN=gtf2 PE=3 SV=1 MIELYDSYSQESRDLHESLGATGLSQLGVVIDADGFLPDGLLSPFTYYLGYEDGKPLYFN QVPVSDFWEILGDNQSACIEDVTQERAVIHYADGMQARLVKQVDWKDLEGRVRQVDHYNR FGACFATTTYSADSEPIMTVYQDVNGQQVLLENHVTGDILLTLPGQSMRYFANKVEFITF FLQDLEIDTSQLIFNTLATPFLVSFHHPDKSGSDVLVWQEPLYDAIPGNMQLILESDNVR TKKIIIPNKATYERALELTDEKYHDQFVHLGYHYQFKRDNFLRRDALILTNSDQIEQVEA IAGALPDVTFRIAAVTEMSSKLLDMLCYPNVALYQNASPQKIQELYQLSDIYLDINHSNE LLQAVRQAFEHNLLILGFNQTVHNRLYIAPDHLFESSEVAALVETIKLALSDVDQMRQAL GKQGQHANYVDLVRYQETMQTVLGG
EMBL CDSAAK75832.1
Sequence length445 AA
Subcellular LocationMembrane (Integral component of membrane)
Additional Information1) DUF1975, a domain of GtfA necessary for the formation of active GtfA-GtfB complex, the Beta-meander add-on domain DUF1975 of GtfA is critical for the recognition of both co-activator GtfB and acceptor PsrP.
2) GtfB functions as a co-activator/chaperone for the full activity of GtfA.
Glycosyltransferase Information
Glycosylation TypeO- (Ser/Thr) linked 
CAZY FamilyGT8
EC Number (BRENDA)2.4.1.-
Mechanism of Glycan TransferSequential
Donor TypeUDP-GlcNAc
Donor SpecificityNucleotide activated sugars
Accessory GT IDProGT75.1, ProGT75.2, ProGT75.3, ProGT75.4, ProGT75.5, ProGT75.6, ProGT75.7
Glycan Information
Glycan transferredMonosaccharide (GlcNAc) 
Method of Glycan IndentificationLTQ Orbitrap MS
Acceptor Subtrate Information
Acceptor Substrate name PsrP (pneumococcal serine-rich repeat protein)
ProGPdb ID ProGP449
Litrature
Year Of Validation2014 
Reference Shi, W.W., Jiang, Y.L., Zhu, F., Yang, Y.H., Shao, Q.Y., Yang, H.B., Ren, Y.M., Wu, H., Chen, Y. and Zhou, C.Z., 2014. Structure of a novel O-linked N-acetyl-D-glucosamine (O-GlcNAc) transferase, GtfA, reveals insights into the glycosylation of pneumococcal serine-rich repeat adhesins. Journal of Biological Chemistry, 289(30), pp.20898-20907.

Corresponding AuthorHefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China.
Reference Jiang, Y.L., Jin, H., Yang, H.B., Zhao, R.L., Wang, S., Chen, Y. and Zhou, C.Z., 2017. Defining the enzymatic pathway for polymorphic O-glycosylation of the pneumococcal serine-rich repeat protein PsrP. Journal of Biological Chemistry, 292(15), pp.6213-6224.

Corresponding Author1 Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China and. 2 Key Laboratory of Structural Biology, Chinese Academy of Science, Hefei, Anhui 230027, China. 3 Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China. 4 Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China.