ProGP686 (Ta0280)
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| ProGP ID | ProGP686 (Ta0280) |
| Validation Status | Characterized |
| Organism Information | |
| Organism Name | Thermoplasma acidophilum DSM 1728 (ATCC 25905) |
| Domain | Archaea |
| Classification | Phylum : Candidatus Thermoplasmatota Class : Thermoplasmata Orders : Thermoplasmatales Family : Thermoplasmataceae Genus : Thermoplasma Species : acidophilum Strain : DSM 1728 |
| Taxonomic ID (NCBI) | 273075 |
| Genome Information | |
| GenBank | NC_00257 |
| Organism Additional Information | It is a thermophilic archaeon grows optimally at pH 2 and 59°C and can multiply between pH 0.5 and 4 & temperature between 40 and 62°C. It is spherical, variable in size which lacks cell wall or a surface layer and delineated by a single double layer membrane. |
| Gene Information | |
| Gene Name | Ta0280 |
| NCBI Gene ID | NC_002578.1 |
| Protein Information | |
| Protein Name | plasma membrane |
| UniProtKB/SwissProt ID | Q9HLE8 |
| NCBI RefSeq | CAC11425.1 |
| UniProtKB Sequence | >tr|Q9HLE8|Q9HLE8_THEAC Hypothetical membrane protein OS=Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165) OX=273075 GN=Ta0280 PE=4 SV=1 MSSLAGLSFAKTPQTGYADIAVSYYGGPAKVPVEVLNFNGAVVASGSGPHLNVSLPFGQY YVYVPNYIGPGSSGNFTIYDAKSVAFNLTSSNVSVSIALTAYPTYGINVSFSGIPNGAIV KFETADDFVFNQTHVINGKYQFQLPIKVPFYAVLDYGTTNHTYLEYLANATSTLSISPNK PDFYGYPNVNSGNIVIIDPVSPYDYTVVPFTNGYFSINASSSDIILVESPGYMPYAFTSS QNGNMIKLSKDVSNVYYNYSLSQNMQTLYQNITFHLNNGTAFPNYFTNSSVGSLYWQMKL DNITESQLVRYFYGLAQNYTLSTFTVNGVNYNITSPPKVTVYSTSNSVTAYVNATYSNMS PVSSLSTINVYVYGTQNTPGSIQYNFNITYNNASLAVSSSNVPLISSYRYVRISPLSSNT MVSLKFSPVQKPVMANNSYTLYWKDMVSDNYVLNNNASNAYYVVPLNQTVYLNASSAYFN PVTNSNDYAQANFTWTINGTTEYSYNVSYKFYDQVNKVSVRVVSPSGGIAYSNFTVIGVS NSTAPMVKFSASLNGKTITSSKNYYRSNNTYLISLSVPQSASVSYSFYGSSLTAQKYSVF LMYSWHFPGQTFIGQNVSYAFQHPSIAPGYLNQYAYANITTEVGNVTHVILHVYVNDTTP PSATFFMMYNGTYIKNPIAGKSIVLSANNTTDPYYPFSDLRFQWKIEYVNGTVAQPSSTT YTNMTPMNNSYVIIQFNTVNSMIISLNVTNPSGISGYYNKTVSMVVQSPRLVVKSIYMPK AAYQGSSSKVYVNVSNLGTMNAYDVNITLYVNGKVVGSTTVSEIKAGSYYNATVTWVPPT SGKLPLYATAEVGNEPSAFVTAGALTQQVSVNPPAYRTPLIVVSVIVVLVVVVYVYYRIR SGGGKSTQKAVQPKTELPKQQQKKK |
| Subcellular Location | Plasma membrane |
| Function | Structural functions and cell stability |
| Glycosylation Status | |
| Glycosylation Type | N- (Asn) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | N516 |
| Technique(s) used for Glycosylation Detection | SDS-PAGE, PAS staining, N-terminal sequencing of proteins transferred to PVDF, Mass spectrometry of tryptic in-gel digests |
| Technique(s) used for Glycosylated Residue(s) Detection | N-terminal sequencing of proteins transferred to PVDF and Mass spectrometry of tryptic in-gel digests. |
| Glycan Information | |
| Glycan Annotation | D-glycero-α-D-galacto-heptose, 6-C-sulfo-D-fucose (6-deoxy-6-C-sulfo-D-galactose, Fuc6S) |
| Technique(s) used for Glycan Identification | MS/MS, 1-D NMR [total correlation spectroscopy (TOCSY) and nuclear Overhauser effect spectroscopy (NOESY)] and 2-D NMR [NOESY, TOCSY, double quantum filtered correlation spectroscopy, H-C heteronuclear single quantum coherence (HSQC) and heteronuclear multiple bond coherence (HMBC)] |
| Literature | |
| Year of Identification | 2012 |
| Reference | Vinogradov, E., Deschatelets, L., Lamoureux, M., Patel, G.B., Tremblay, T.L., Robotham, A., Goneau, M.F., Cummings-Lorbetskie, C., Watson, D.C., Brisson, J.R. and Kelly, J.F., 2012. Cell surface glycoproteins from Thermoplasma acidophilum are modified with an N-linked glycan containing 6-C-sulfofucose. Glycobiology, 22(9), pp.1256-1267. |