| ProGT ID | ProGT1.1 (WbpM) |
| Organism Information | |
| Organism Name | Pseudomonas aeruginosa 1244 |
| Domain | Bacteria |
| Classification | Phylum : Proteobacteria Class : Gammaproteobacteria Orders : Pseudomonadales Family : Pseudomanadaceae Genus : Pseudomonas Species : aeruginosa |
| Taxonomic ID (NCBI) | 287 |
| Genome Information | |
| Gene Bank | NC_002516.2 |
| Gene Information | |
| Gene Name | wbpM |
| NCBI Reference Sequence | AF508154 |
| Protein information | |
| Protein Name | WbpM |
| UniProtKB/ SwissProt ID | Q7X3T1 |
| NCBI Ref Seq | WP_003143571.1 |
| UniProtKB Sequence | >tr|Q7X3T1|Q7X3T1_PSEAI UDP-GlcNAc C6 dehydratase OS=Pseudomonas aeruginosa GN=wbpM PE=4 SV=1 MLDNLRMKLLGLPRRYKRMLQVAADVSLVWLSLWLAFLVRLGTEDMISPFGGHAWLFIAA PLVAIPLFIRFGMYRAVMRYLGNDALIAIAKAVTISALVLSLLVYWYRSPPAVVPRSLVF NYWWLSMLLIGGLRLAMRQYFMGDWYSAVQSVPFLNRQDGLPRVAIYGAGAAGNQLVAAL RLGRAMRPVAFIDDDKQIANRVIAGLRVYTAKHIRQMIDETGAQEVLLAIPSATRARRRE ILESLEPFPLHVRSMPGFMDLASGRVKVDDLQEVDIADLLGRDSVAPRKELLERCIRGQV VMVTGAGGSIGSELCRQIMSCSPSVLILFEHSEYNLYSIHQELERRIKRESLSVNLLPIL GSVRNPERLVDVMRTWKVNTVYHAAAYKHVPIVEHNIAEGVLNNVIGTLHAVQAAVQVGV QNFVLISTDKAVRPTNVMGSTKRLAEMVLQALSNESAPVLFGDRKDVHHVNKTRFTMVRF GNVLGSSGSVIPLFREQIKRGGPVTVTHPSITRYFMTIPEAAQLVIQAGSMGQGGDVFVL DMGPPVKILELAEKMIHLSGLSVRSERSPHGDIAIEFSGLRPGEKLYEELLIGDNVNPTD HPMIMRANEEHLSWEAFKVVLEQLLAAVEKDDYSRVRQLLRETVSGYAPDGEIVDWIYRQ RRREP |
| EMBL CDS | AAP57848.1 |
| Sequence length | 665 AA |
| Subcellular Location | Membrane (Integral component of membrane) |
| Glycosylation Information | |
| CAZY Family | Non-GT |
| EC Number (BRENDA) | 1.1.1.- |
| Acceptor Substrate Specificity | UDP-GlcNAc |
| Experimental Validation | In vivo |
| Product | UDP-QuiNAc |
| Function in Glycosylation pathway | 1) WbpM is a bifunctional UDP-GlcNAc C6 dehydratase/C4 reductase essential for the nucleotide precursor UDP-QuiNAc synthesis. |
| Additional Information | 1) Expression of O-antigen biosynthesis cluster of Escherichia coli O157:H7 in strain P. aeruginosa 1244 resulted in the production of pilin that contained both the endogenous Pseudomonas as well as the Escherichia O157 O-antigens. |
| Litrature | |
| Year Of Validation | 2002 |
| Reference | DiGiandomenico, A., Matewish, M.J., Bisaillon, A., Stehle, J.R., Lam, J.S. and Castric, P., 2002. Glycosylation of Pseudomonas aeruginosa 1244 pilin: glycan substrate specificity. Molecular microbiology, 46(2), pp.519-530. |
| Corresponding Author | Department of Biological Sciences, Duquesne University, Pittsburg, PA 15282, USA. |
| Reference | Horzempa, J., Dean, C.R., Goldberg, J.B. and Castric, P., 2006. Pseudomonas aeruginosa 1244 pilin glycosylation: glycan substrate recognition. Journal of bacteriology, 188(12), pp.4244-4252. |
| Corresponding Author | 1 Department of Biological Sciences, Duquesne University, 600 Forbes Ave., Pittsburgh, PA 15282, USA. |