ProGP1133 (SCO4256)
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| ProGP ID | ProGP1133 (SCO4256) |
| Validation Status | Characterized |
| Organism Information | |
| Organism Name | Streptomyces coelicolor |
| Domain | Bacteria |
| Classification | Phylum : Actinobacteria Class : Actinomycetia Orders : Streptomycetales Family : Streptomycetaceae Genus : Streptomyces Species : coelicolor |
| Taxonomic ID (NCBI) | 100226 |
| Genome Information | |
| GenBank | AL645882.2 |
| EMBL | AL939119 |
| Organism Additional Information | Streptomyces coelicolor, a filamentous, high G-C, gram-positive bacteria and are responsible for much of the break down of organic material in the soil as well as the “earthy” smell of soil. Colonies of Streptomyces coelicolor release pigments that are blue/green in alkali and red in acidic conditions, thereby giving the bacterial colonies those colors under the respective conditions |
| Gene Information | |
| Gene Name | sco4256 |
| NCBI Gene ID | NC_003888.3 |
| Protein Information | |
| Protein Name | SCO4256 |
| UniProtKB/SwissProt ID | Q9L0N5 |
| NCBI RefSeq | CAB77350 |
| UniProtKB Sequence | >CAB77350.1 putative hydrolytic protein [Streptomyces coelicolor A3(2)] MSLWTSLEPASATVDPGSSTRVRLRVRNTGDVVDEYRFEPVGDVAPWTTVEPQTLRLYPGTTGTVELTFAPPRTSDAVAGPNPYAVRILPTEHPDAVTVPEGNLTITAFTEVRAELVPPTVKGRFRGRPRLAVDNLGNTKVTASIAGSDHGDHLSYEVRPGNVQIEPGRAAFVETTLKPKRIIWFGAKEERPYTLAVRRSGVDPTEIEGTYVQRGFLPRWLATFFGIFLALAIAFVMIWIAYKPQVRTSATEQTEQAGVALAPSPSATPETLPSSAESAPVEQPQVQQPPGTQEPEKDKGDGDGGDKGGGGGGGGESKKPKPPVRTAATAVKEVAARSEGRHICYRAYVADQGWQAPVCDGAEAGTVGKNLPIKALNIAVSGTKGVTGNGAHVVEAWLNGSKWESVPDQTDMYLGSTKEAVSPMEGFTLKTVEGTVCPNTHVKDKGWLKQGCTEPGAWRYFGSNMENDRLQLEAVRITV |
| Subcellular Location | Membrane protein |
| Function | Solute binding, polysaccharide hydrolases, ABC transporters, enzyme function, cell wall biosynthesis, and maintaining membrane integrity |
| Glycosylation Status | |
| Glycosylation Type | O- (Ser) linked |
| Experimentally Predicted Glycosites | S-317 |
| Experimentally Validated Glycosite(s) in Full Length Protein | S-317 |
| Experimentally Validated Glycosite(s ) in Mature Protein | >CAB77350.1 putative hydrolytic protein [Streptomyces coelicolor A3(2)] MSLWTSLEPASATVDPGSSTRVRLRVRNTGDVVDEYRFEPVGDVAPWTTVEPQTLRLYPGTTGTVELTFAPPRTSDAVAGPNPYAVRILPTEHPDAVTVPEGNLTITAFTEVRAELVPPTVKGRFRGRPRLAVDNLGNTKVTASIAGSDHGDHLSYEVRPGNVQIEPGRAAFVETTLKPKRIIWFGAKEERPYTLAVRRSGVDPTEIEGTYVQRGFLPRWLATFFGIFLALAIAFVMIWIAYKPQVRTSATEQTEQAGVALAPSPSATPETLPSSAESAPVEQPQVQQPPGTQEPEKDKGDGDGGDKGGGGGGGGES*(317)KKPKPPVRTAATAVKEVAARSEGRHICYRAYVADQGWQAPVCDGAEAGTVGKNLPIKALNIAVSGTKGVTGNGAHVVEAWLNGSKWESVPDQTDMYLGSTKEAVSPMEGFTLKTVEGTVCPNTHVKDKGWLKQGCTEPGAWRYFGSNMENDRLQLEAVRITV |
| Glycosite(s) Annotated Protein Sequence | KGGGGGGGGESKKPKPPVRTA |
| Technique(s) used for Glycosylation Detection | SDS-PAGE and lectin western blotting |
| Literature | |
| Year of Identification | 2019 |
| Reference | Keenan, T., Dowle, A., Bates, R. and Smith, M.C., 2019. Characterization of the Streptomyces coelicolor glycoproteome reveals glycoproteins important for cell wall biogenesis. Mbio, 10(3), pp.e01092-19. |
| Corresponding Author | Margaret C. M. Smith |
| Contact | Department of Biology, University of York, York, United Kingdom |