ProGP121 (Surface layer glycoprotein Sat B)
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| ProGP ID | ProGP121 (Surface layer glycoprotein Sat B) |
| Validation Status | Characterized |
| Organism Information | |
| Organism Name | Aneurinibacillus thermoaerophilus DSM 10155 |
| Domain | Bacteria |
| Classification | Phylum : Firmicutes Class : Bacilli Orders : Bacillales Family : Paenibacillaceae Genus : Aneurinibacillus Species : thermoaerophilus Strain : DSM 10155 |
| Taxonomic ID (NCBI) | 143495 |
| Genome Information | |
| GenBank | AY395579 |
| EMBL | AY395579 |
| Gene Information | |
| Gene Name | sat B |
| Protein Information | |
| Protein Name | Surface-layer glycoprotein Sat B |
| UniProtKB/SwissProt ID | Q6TL21 |
| EMBL-CDS | AAS44592.1 |
| UniProtKB Sequence | >tr|Q6TL21|Q6TL21_ANETH Surface-layer glycoprotein SatB OS=Aneurinibacillus thermoaerophilus GN=satB PE=3 SV=1 MKTNKALKVLTTSALLASVAAPFGAGVVSANITNLVNKVLNVKSNYDGEQSASHTLTVKE DDIAFDQTSGETFRVVLPSGVKWKTAPANTPTAKFDLRSDQVLEVTVKGTNTIADDEQVS IPLNFKVDGATGELKLKVESMDSTITSGEYVFAIVGSGSTTAAVDNVKNIGKSGKAGTIR VEETAIGAVADGRNHKVKLKLPSNFRWVTTGTDKTEISTSGGFTSASIVTGESNDNVLVL DVATATGQGRGVLYVTPYIKADKDASYGDVSVNIRGPDFSDSDVVVAKYGDWGATIKVEK VEEVLAGKYDENTRTAKITIEESVPGSFVKGRDIDVELPSWVKVTDIKEFTTSGFTTGAP TSGSVDIDGTDNKISIPLGETGGSSTAKIEFKLELSVQADKTGDIEAVISGGGVAEQKAV IAKAVAPVSVALDGEASKVKIGAQAQDAPNIIITELKKGAIEKTTENGSTTNGTIEVKLP DGVKFASVPKVEVVEGDGTIDVDNVRRINNDNVLTIPVKGESVSKPMKIKLSNVKLTVDR TVPEGDIELKVGGNAIVENYKSGKGWLKGNKQNGSSDALDAGEFNTEHAAKVVVAKTVTP APTDTTATNIVFKLNSKTYTVDGKEFEMDAAPLVGWDRAYLPVRFAANALNVSDDQIVWD DKTSTFTIFKGDRVISGKVGDKFLTVNGAKVPMDVPVWRNKEKTNNRVMVPIRYLANALN AEIEWNKETSEITIQAKK |
| Sequence length | 738 AA |
| Subcellular Location | Surface |
| Glycosylation Status | |
| Glycosylation Type | O- (Ser/Thr) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | T471 and/or S69 |
| Experimentally Validated Glycosite(s ) in Mature Protein | T471 and /or S69 |
| Glycosite(s) Annotated Protein Sequence | >tr|Q6TL21|Q6TL21_ANETH Surface-layer glycoprotein SatB OS=Aneurinibacillus thermoaerophilus GN=satB PE=3 SV=1 MKTNKALKVLTTSALLASVAAPFGAGVVSANITNLVNKVLNVKSNYDGEQSASHTLTVKE DDIAFDQTS*(69)GETFRVVLPSGVKWKTAPANTPTAKFDLRSDQVLEVTVKGTNTIADDEQVS IPLNFKVDGATGELKLKVESMDSTITSGEYVFAIVGSGSTTAAVDNVKNIGKSGKAGTIR VEETAIGAVADGRNHKVKLKLPSNFRWVTTGTDKTEISTSGGFTSASIVTGESNDNVLVL DVATATGQGRGVLYVTPYIKADKDASYGDVSVNIRGPDFSDSDVVVAKYGDWGATIKVEK VEEVLAGKYDENTRTAKITIEESVPGSFVKGRDIDVELPSWVKVTDIKEFTTSGFTTGAP TSGSVDIDGTDNKISIPLGETGGSSTAKIEFKLELSVQADKTGDIEAVISGGGVAEQKAV IAKAVAPVSVALDGEASKVKIGAQAQDAPNIIITELKKGAIEKTTENGSTT*(471)NGTIEVKLP DGVKFASVPKVEVVEGDGTIDVDNVRRINNDNVLTIPVKGESVSKPMKIKLSNVKLTVDR TVPEGDIELKVGGNAIVENYKSGKGWLKGNKQNGSSDALDAGEFNTEHAAKVVVAKTVTP APTDTTATNIVFKLNSKTYTVDGKEFEMDAAPLVGWDRAYLPVRFAANALNVSDDQIVWD DKTSTFTIFKGDRVISGKVGDKFLTVNGAKVPMDVPVWRNKEKTNNRVMVPIRYLANALN AEIEWNKETSEITIQAKK |
| Sequence Around Glycosites (21 AA) | IEKTTENGSTTNGTIEVKLPD
KEDDIAFDQTSGETFRVVLPS |
| Technique(s) used for Glycosylation Detection | Periodic acid-Schiff (PAS) staining |
| Technique(s) used for Glycosylated Residue(s) Detection | Modified Edman degradation |
| Glycan Information | |
| Glycan Annotation | Linkage: β-GalNAc-Ser/Thr. Total carbohydrate content of 15%. Disaccharide repeating unit composed of L-rhamnose and D-glycero-D-manno-heptose residues: →4)-α-L-Rhap-(1→3)-β-D-glycero-D-manno-Hepp-(1→ and β-D-GalpNAc-(1→O)-Ser/Thr linkage. Equal amounts of the complete core structure α-L-Rhap-(1→3)-α-L-Rhap-(1→3)-β-D-GalpNAc-(1→O)-Ser/Thr and the truncated forms α-L-Rhap-(1→3)-β-D-GalpNAc-(1→O)-Ser/Thr and β-D-GalpNAc-(1→O)-Ser/Thr are present. The different cores were substituted with varying numbers of disaccharide repeating units. |
| BCSDB ID | 23143943 |
| GlyTouCan | G56797IU G29447EH |
| Literature | |
| Year of Identification | 1995 |
| Year of Identification Month Wise | 1995.12.25 |
| Year of Validation | 1999 |
| Reference | Messner, P., Steiner, K., Zarschler, K. and Schäffer, C., 2008. S-layer nanoglycobiology of bacteria. Carbohydrate Research, 343(12), pp.1934-1951. |
| Corresponding Author | Paul Messner |
| Contact | University of Natural Resources and Life Sciences, Vienna, Center for NanoBiotechnology A-1180 Vienna, Gregor-Mendel-Strasse 33, Austria. |
| Reference | Kneidinger, B., Graninger, M., Puchberger, M., Kosma, P. and Messner, P., 2001. Biosynthesis of Nucleotide-activatedd-glycero-d-manno-Heptose. Journal of Biological Chemistry, 276(24), pp.20935-20944. |
| Corresponding Author | Paul Messner |
| Contact | University of Natural Resources and Life Sciences, Vienna, Center for NanoBiotechnology A-1180 Vienna, Gregor-Mendel-Strasse 33, Austria. |
| Reference | Wugeditsch, T., Zachara, N.E., Puchberger, M., Kosma, P., Gooley, A.A. and Messner, P., 1999. Structural heterogeneity in the core oligosaccharide of the S-layer glycoprotein from Aneurinibacillus thermoaerophilus DSM 10155. Glycobiology, 9(8), pp.787-795. |
| Corresponding Author | Paul Messner |
| Contact | University of Natural Resources and Life Sciences, Vienna, Center for NanoBiotechnology A-1180 Vienna, Gregor-Mendel-Strasse 33, Austria. |
| Reference | Kosma, P., Wugeditsch, T., Christian, R., Zayni, S. and Messner, P., 1995. Glycan structure of a heptose-containing S-layer glycoprotein of Bacillus thermoaerophilus. Glycobiology, 5(8), pp.791-796. |
| Corresponding Author | Paul Messner |
| Contact | University of Natural Resources and Life Sciences, Vienna, Center for NanoBiotechnology A-1180 Vienna, Gregor-Mendel-Strasse 33, Austria. |