ProGP122 (Surface layer glycoprotein SatA)
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| ProGP ID | ProGP122 (Surface layer glycoprotein SatA) |
| Validation Status | Characterized |
| Organism Information | |
| Organism Name | Aneurinibacillus thermoaerophilus L420-91T (DSM 10154) and GS4-97 |
| Domain | Bacteria |
| Classification | Phylum : Firmicutes Class : Bacilli Orders : Bacillales Family : Paenibacillaceae Genus : Aneurinibacillus Species : thermoaerophilus Strain : L420-91T (DSM 10154) and GS4-97 |
| Taxonomic ID (NCBI) | 143495 |
| Genome Information | |
| GenBank | AY395578 |
| EMBL | AY395578 |
| Gene Information | |
| Gene Name | sat A |
| Protein Information | |
| Protein Name | Surface-layer glycoprotein SatA |
| UniProtKB/SwissProt ID | Q6TL22 |
| EMBL-CDS | AAS44591.1 |
| UniProtKB Sequence | >tr|Q6TL22|Q6TL22_ANETH Surface-layer glycoprotein SatA OS=Aneurinibacillus thermoaerophilus GN=satA PE=3 SV=1 MKTNKALKVLTTSALLASVAAPFGAGVVSANSTNLVDKRVQVGSNSEINGTSHLIVKEDD KKFRVSTGDTFRVELPAGTEWKFSDSEFYVDGSGNVYSSESSASPDTKAKVNVISSRTIE VRPLGNGFKSGDPDSKNAINIPLHVKVNGASGELKLKIDSMNSTVTSGEYVFATVNTGNT RAVVDTVRYIGKNGIGGQIRIDENFAGAISAGTHSLKLKLPSNFEWDDMSSGEIKFGGSF FGNAKVDGAIIGNGDNTLTIPVKISQSTSGATPGYIYITPKIKAKSDAAKGTDVTVNISG GDFSDADLTVAKYGDYDANIKVEKVEELVAGKFDEYTKTAKITIEESVPGSFYKDRDIDV KFPSWVKVVEVSDWSTSGVKANKPKNIDGTDDEFSIKIEEPTTKENTTPGRIEFKVRLSI DANKTGDIEATVSGGGIKEQKAVVAKAIAPAAVSVEGDTSKLKIGAQDQEGPTFYITEAK KGVFEKNVEGPSGNGEIIVRLASGVKFSKVPKVEVVEGDGPIDTSNVRRIENDNALAIPI KGESTSKPMKLKVSDVRLTLDRTVPEGDIEAKIGGSAVVENYVKDSKGWVGTGLYTGTDN KNPGTFNTQHAVTFKIGSTITPAPSDTTAKNIVFKLNSKTYTVDGKEIEMDAAPLVGWDR AYLPVRFAANALNVSDDQIIWDDKTSTFTIFKGDRVISGKVGDKFLTINGVKTPMDVPVW RNKEKTNNRVMVPIRYLANALNAEINWNKETSEITIQVK |
| Sequence length | 759 AA |
| Subcellular Location | Surface |
| Glycosylation Status | |
| Glycosylation Type | O- (Ser/Thr) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | T67 |
| Experimentally Validated Glycosite(s ) in Mature Protein | T67 |
| Glycosite(s) Annotated Protein Sequence | >tr|Q6TL22|Q6TL22_ANETH Surface-layer glycoprotein SatA OS=Aneurinibacillus thermoaerophilus GN=satA PE=3 SV=1 MKTNKALKVLTTSALLASVAAPFGAGVVSANSTNLVDKRVQVGSNSEINGTSHLIVKEDD KKFRVST*(67)GDTFRVELPAGTEWKFSDSEFYVDGSGNVYSSESSASPDTKAKVNVISSRTIE VRPLGNGFKSGDPDSKNAINIPLHVKVNGASGELKLKIDSMNSTVTSGEYVFATVNTGNT RAVVDTVRYIGKNGIGGQIRIDENFAGAISAGTHSLKLKLPSNFEWDDMSSGEIKFGGSF FGNAKVDGAIIGNGDNTLTIPVKISQSTSGATPGYIYITPKIKAKSDAAKGTDVTVNISG GDFSDADLTVAKYGDYDANIKVEKVEELVAGKFDEYTKTAKITIEESVPGSFYKDRDIDV KFPSWVKVVEVSDWSTSGVKANKPKNIDGTDDEFSIKIEEPTTKENTTPGRIEFKVRLSI DANKTGDIEATVSGGGIKEQKAVVAKAIAPAAVSVEGDTSKLKIGAQDQEGPTFYITEAK KGVFEKNVEGPSGNGEIIVRLASGVKFSKVPKVEVVEGDGPIDTSNVRRIENDNALAIPI KGESTSKPMKLKVSDVRLTLDRTVPEGDIEAKIGGSAVVENYVKDSKGWVGTGLYTGTDN KNPGTFNTQHAVTFKIGSTITPAPSDTTAKNIVFKLNSKTYTVDGKEIEMDAAPLVGWDR AYLPVRFAANALNVSDDQIIWDDKTSTFTIFKGDRVISGKVGDKFLTINGVKTPMDVPVW RNKEKTNNRVMVPIRYLANALNAEINWNKETSEITIQVK |
| Sequence Around Glycosites (21 AA) | KEDDKKFRVSTGDTFRVELPA |
| Technique(s) used for Glycosylation Detection | Periodic acid-Schiff (PAS)-staining |
| Technique(s) used for Glycosylated Residue(s) Detection | N-terminal sequencing and C-13 NMR |
| Glycan Information | |
| Glycan Annotation | Linkage: β-GalNAc-Ser/Thr. Hexasaccharide repeating unit (an average of 15 repeats) with the constituents D-rhamnose and 3-N-acetyl-D-fucosamine (3-acetamido-3,6-dideoxy-D-galactose); and β-D-GalNAc-Ser/Thr linkage. An O-methyl group on the non-reducing terminal rhamnose. |
| BCSDB ID | 23142 |
| GlyTouCan | G90479EZ |
| Literature | |
| Year of Identification | 1995 |
| Year of Identification Month Wise | 1995.12.25 |
| Year of Validation | 1999 |
| Reference | Messner, P., Steiner, K., Zarschler, K. and Schäffer, C., 2008. S-layer nanoglycobiology of bacteria. Carbohydrate Research, 343(12), pp.1934-1951. |
| Corresponding Author | Paul Messner |
| Contact | University of Natural Resources and Life Sciences, Vienna, Center for NanoBiotechnology A-1180 Vienna, Gregor-Mendel-Strasse 33, Austria. |
| Reference | Pfoestl, A., Hofinger, A., Kosma, P. and Messner, P., 2003. Biosynthesis of dTDP-3-acetamido-3, 6-dideoxy-α-D-galactose in Aneurinibacillus thermoaerophilus L420-91T. Journal of Biological Chemistry, 278(29), pp.26410-26417. |
| Corresponding Author | Paul Messner |
| Contact | University of Natural Resources and Life Sciences, Vienna, Center for NanoBiotechnology A-1180 Vienna, Gregor-Mendel-Strasse 33, Austria. |
| Reference | Schäffer, C., Müller, N., Christian, R., Graninger, M., Wugeditsch, T., Scheber, A. and Messner, P., 1999. Complete glycan structure of the S-layer glycoprotein of Aneurinibacillus thermoaerophilus GS4-97 cg. Glycobiology, 9(4), pp.407-414. |
| Corresponding Author | Paul Messner |
| Contact | University of Natural Resources and Life Sciences, Vienna, Center for NanoBiotechnology A-1180 Vienna, Gregor-Mendel-Strasse 33, Austria. |
| Reference | Kosma, P., Wugeditsch, T., Christian, R., Zayni, S. and Messner, P., 1995. Glycan structure of a heptose-containing S-layer glycoprotein of Bacillus thermoaerophilus. Glycobiology, 5(8), pp.791-796. |
| Corresponding Author | Paul Messner |
| Contact | University of Natural Resources and Life Sciences, Vienna, Center for NanoBiotechnology A-1180 Vienna, Gregor-Mendel-Strasse 33, Austria. |