ProGP1229 (GkFlaA1)
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| ProGP ID | ProGP1229 (GkFlaA1) |
| Validation Status | Characterized |
| Organism Information | |
| Organism Name | Geobacillus kaustophilus HTA426 |
| Domain | Bacteria |
| Classification | Phylum : Firmicutes Class : Bacilli Orders : Bacillales Family : Bacillaceae Genus : Geobacillus Species : kaustophilus Strain : HTA426 |
| Taxonomic ID (NCBI) | 235909 |
| Genome Information | |
| GenBank | BA000043.1 |
| Gene Information | |
| Gene Name | acm2 |
| Protein Information | |
| Protein Name | GkFlaA1 |
| NCBI RefSeq | BAD77427.1 |
| UniProtKB Sequence | >BAD77427.1 flagellar protein [Geobacillus kaustophilus HTA426] MRINHNIQALNAYRNLAANQSSISKNLERLSSGLRINRAADDAAGLAISEKMRSQIRGLQMAERNALDAI SLIQTAEGALNEVHSILQRMRELAVQAANDTLEPSDRAAIQAEVDQLTKEIDRIADTTQFNQKILFSGSP EGRAFAQANSPAIRYAGNGTWQGVVTATPTDPAKVVLDFSKNLGMLTRDQIDGQTLTINGKTYQIDTKND GLTNTGHIAVNVSWIATPANDSDAVTNINNLLNALKNAIVANDSTFSSTSLITNASDSGTNSDGVIVNGR LTLTTANNMAPKDAALIGVASSFPSTQLTCLDPVNLTSTVTSLVAKDQALVSESLTLTFEDVPKNGDVLK IDNLTITFSDSTASGYTWSGGQGTATINVSGKTVFDVLDDIKQVLGDAKADGINQPVRALTAFSVVGSSL VLSTDRTDDGKPFGSANGLEVQIIDNDFEANKGKTLKLDMQIGANESETLSLDVGVMDAASLGLARMADH TTILNTGVNAEAGIDVSSSAQAAHAAITIIDQAINKVSEERGKLGAVQNRLEHTINNLTTANENLTAAES RIRDTDMAMEMAEFTKNNILTQAAQAMLAQSNQLPQGILQLLKS |
| Function | Required for motility |
| Glycosylation Status | |
| Glycosylation Type | O- (Ser/Thr) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | T270 and T276 |
| Experimentally Validated Glycosite(s ) in Mature Protein | >BAD77427.1 flagellar protein [Geobacillus kaustophilus HTA426] MRINHNIQALNAYRNLAANQSSISKNLERLSSGLRINRAADDAAGLAISEKMRSQIRGLQMAERNALDAI SLIQTAEGALNEVHSILQRMRELAVQAANDTLEPSDRAAIQAEVDQLTKEIDRIADTTQFNQKILFSGSP EGRAFAQANSPAIRYAGNGTWQGVVTATPTDPAKVVLDFSKNLGMLTRDQIDGQTLTINGKTYQIDTKND GLTNTGHIAVNVSWIATPANDSDAVTNINNLLNALKNAIVANDSTFSSTSLITNASDSGT*(270)NSDGVIVNGR LTLTTANNMAPKDAALIGVASSFPSTQLTCLDPVNLTSTVTSLVAKDQALVSESLTLTFEDVPKNGDVLK IDNLTITFSDSTASGYTWSGGQGTATINVSGKTVFDVLDDIKQVLGDAKADGINQPVRALTAFSVVGSSL VLSTDRTDDGKPFGSANGLEVQIIDNDFEANKGKTLKLDMQIGANESETLSLDVGVMDAASLGLARMADH TTILNTGVNAEAGIDVSSSAQAAHAAITIIDQAINKVSEERGKLGAVQNRLEHTINNLTTANENLTAAES RIRDTDMAMEMAEFTKNNILTQAAQAMLAQSNQLPQGILQLLKS |
| Glycosite(s) Annotated Protein Sequence | SLITNASDSGTNSDGVIVNGR SDGVIVNGRLTLTTANNMAPK |
| Technique(s) used for Glycosylation Detection | LC–MS/MS and Western blotting |
| Glycan Information | |
| Glycan Annotation | N-acetylneuraminic acid |
| Technique(s) used for Glycan Identification | LC–MS/MS and Western blooting |
| Protein Glycosylation linked (PGL) gene(s) | |
| OST ProGT ID | ProGT135 (Maf) |
| Literature | |
| Year of Identification | 2021 |
| Reference | Khairnar, A., Sunsunwal, S., Babu, P. and Ramya, T.N.C., 2021. Novel serine/threonine-O-glycosylation with N-acetylneuraminic acid and 3-deoxy-D-manno-octulosonic acid by bacterial flagellin glycosyltransferases. Glycobiology, 31(3), pp.288-306. |
| Corresponding Author | T N C Ramya |
| Contact | Department of Protein Science and Engineering, CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh 160036, India |